Objective:To test two water soluble extracts(aqueous and ethanolic) obtained from the leaves of Vitex doniana in normal and streptozotocin-induced diabetic rats for their effects on pancreatic endocrine tissues and se...Objective:To test two water soluble extracts(aqueous and ethanolic) obtained from the leaves of Vitex doniana in normal and streptozotocin-induced diabetic rats for their effects on pancreatic endocrine tissues and serum marker enzymes for a period of 21 d.Methods:A total of 55 rate divided into 11 groups of 5 rats each were assigned into diabetic and non-diabetic- groups and fallowed by a daily administration of ethanolic and aqueous extracts for21 d.Group 1 was the normal control while group 7 was treated with standard drug.Results:The histopathological studies of the diabetic rats indicated increase in the volume density of islets,percent of β-cells and size of islet in the groups that received the plant extracts,which suggested regeneration of β-cells along with p-eells repairs,as compared with the non-treated diabetic control which showed complete degeneration of the islet cells.There was significant reduction(P<0.05) in the serum activities of marker enzymes,alanine amimilransferase.aspartate aminotransferase and alkaline phosphatase in diabetes treated rats,whereas an insignificant increase(P>0.01) in the serum activities of marker enzymes was observed for non-diabetic treated rats.Results of total bilirubin,direct bilirubin and unconjugated bilirubin showed that diabetic control group was significantly higher(P<0.05) in total bilirubin and unconjugated bilirubin compared with treated groups while non-diabetic treated groups showed no significant increase(P>0.01) in total bilirubin and direct bilirubin compared with the normal control.Conclusion:This herbal therapy appears to bring about repair/regeneration of the endocrine pancreas and hepatic cells protection in the diabetic rat.展开更多
基金Supported by the Department of Biochemistry,Faculty of Science,Ahmadu Bello University,Zaria(Grant No.ABUBCH-0102-0005)
文摘Objective:To test two water soluble extracts(aqueous and ethanolic) obtained from the leaves of Vitex doniana in normal and streptozotocin-induced diabetic rats for their effects on pancreatic endocrine tissues and serum marker enzymes for a period of 21 d.Methods:A total of 55 rate divided into 11 groups of 5 rats each were assigned into diabetic and non-diabetic- groups and fallowed by a daily administration of ethanolic and aqueous extracts for21 d.Group 1 was the normal control while group 7 was treated with standard drug.Results:The histopathological studies of the diabetic rats indicated increase in the volume density of islets,percent of β-cells and size of islet in the groups that received the plant extracts,which suggested regeneration of β-cells along with p-eells repairs,as compared with the non-treated diabetic control which showed complete degeneration of the islet cells.There was significant reduction(P<0.05) in the serum activities of marker enzymes,alanine amimilransferase.aspartate aminotransferase and alkaline phosphatase in diabetes treated rats,whereas an insignificant increase(P>0.01) in the serum activities of marker enzymes was observed for non-diabetic treated rats.Results of total bilirubin,direct bilirubin and unconjugated bilirubin showed that diabetic control group was significantly higher(P<0.05) in total bilirubin and unconjugated bilirubin compared with treated groups while non-diabetic treated groups showed no significant increase(P>0.01) in total bilirubin and direct bilirubin compared with the normal control.Conclusion:This herbal therapy appears to bring about repair/regeneration of the endocrine pancreas and hepatic cells protection in the diabetic rat.
