This study investigated the effects on the reproductive system of balb-c mice exposed to water collected from different sources near to a deactivated fertilizer waste depositary. Eighty male mice were separated in fou...This study investigated the effects on the reproductive system of balb-c mice exposed to water collected from different sources near to a deactivated fertilizer waste depositary. Eighty male mice were separated in four groups: Group A (mineral water);Group B (water from the water treatment station);Group C (water from Cubatao city);Group D (water from the waste depositary region). They were exposed to water since they were weaned until they reached sexual maturity, then they were coupled with females in reproductive age and after this mating time they were sacrificed. The evaluated parameters were testicle weight, sperm analysis, pregnancy rate, sex ratio of the offspring and Sertoli cell count. The analysis of the water did not show presence of pollutants in the Group “A” and Group “C” water. Group “B” showed low level of cadmium, 3.58 ± 0.50 μg/L. Group “D” showed the presence of PAH’s and high levels of lead (221 ± 16 μg/L), cadmiun (12.6 ± 1.2 μg/L) and mercury (5.3 ± 1.1 μg/L). The tests of Levene and Kolmogorov-Smirnov were employed to verify the homogeneity of the variances and the tack to the normal curve, respectively. ANOVA was used for parametric tests and Kruskall-Wallis was used for non-parametric tests, while Turkey tests were employed for multiple comparisons. There were no differences between groups in testicle weight, sperm analysis, pregnancy rate and Sertoli cell count. There was a significant reduction in sex-ratio of the offspring in Group B. This alteration cannot be explained by the cadmium levels in Group B water. In the present study we cannot associate the exposition to contaminated water from the waste depositary and reproductive alterations.展开更多
A comprehensive understanding of the complex biogeochemical interactions between organic matter and persistent contaminants in the suspended matter is vital for ecoefficient estuary recovery.However,little is known re...A comprehensive understanding of the complex biogeochemical interactions between organic matter and persistent contaminants in the suspended matter is vital for ecoefficient estuary recovery.However,little is known regarding aeration effects in suspended particulate aggregates.Therefore,this study aimed to investigate the effects of aeration on the suspended matter from a Tropical and Eutrophic estuarine environment.Anoxic water with 60 g/L of suspended particulate matter(SPM)was collected from Guanabara Bay,Rio de Janeiro,Brazil,transferred to experimental boxes and aerated for 61 days.SPM aggregates monitoring included abiotic variables measurements and,determination of total organic matter(TOM),biopolymers composition,bacterial activity,trace metals,and polycyclic aromatic hydrocarbons(PAHs)concentrations.The aeration enhanced dissolved oxygen(DO)concentration and the redox potential(Eh).However,from days 0 to 61 the predominant bacterial activities were denitrification and fermentation.Electron transport system activity increased after day 10,and aerobic activity was detected after day 19.In summary,aeration increased aerobic bacterial activity,lipids(LIP)and trace metal concentrations,although diminished protein/carbohydrate ratio and PAH concentration.Trace metals concentration(Ni,Pb,Cu,Cr,Mn,and Fe)were the highest on day 19 when the p H was 5.9.Copper presented toxic values(Cu>20.0μg/g).The p H showed a strong negative correlation with Eh(r=-0.94;p<0.001).Acidic environment(p H≤5.9)in marine ecosystems with high loads of toxic trace metals is unsafe for biota.Therefore,managers must be aware of the environmental and biological risks of introducing the aeration technique into a eutrophic marine environment.展开更多
文摘This study investigated the effects on the reproductive system of balb-c mice exposed to water collected from different sources near to a deactivated fertilizer waste depositary. Eighty male mice were separated in four groups: Group A (mineral water);Group B (water from the water treatment station);Group C (water from Cubatao city);Group D (water from the waste depositary region). They were exposed to water since they were weaned until they reached sexual maturity, then they were coupled with females in reproductive age and after this mating time they were sacrificed. The evaluated parameters were testicle weight, sperm analysis, pregnancy rate, sex ratio of the offspring and Sertoli cell count. The analysis of the water did not show presence of pollutants in the Group “A” and Group “C” water. Group “B” showed low level of cadmium, 3.58 ± 0.50 μg/L. Group “D” showed the presence of PAH’s and high levels of lead (221 ± 16 μg/L), cadmiun (12.6 ± 1.2 μg/L) and mercury (5.3 ± 1.1 μg/L). The tests of Levene and Kolmogorov-Smirnov were employed to verify the homogeneity of the variances and the tack to the normal curve, respectively. ANOVA was used for parametric tests and Kruskall-Wallis was used for non-parametric tests, while Turkey tests were employed for multiple comparisons. There were no differences between groups in testicle weight, sperm analysis, pregnancy rate and Sertoli cell count. There was a significant reduction in sex-ratio of the offspring in Group B. This alteration cannot be explained by the cadmium levels in Group B water. In the present study we cannot associate the exposition to contaminated water from the waste depositary and reproductive alterations.
基金supported by Conselho Nacional de Desenvolvimento Científico e Tecnológico, Brazil (CNPq Nos. 160053/2008-0 and 380487/2007-1)
文摘A comprehensive understanding of the complex biogeochemical interactions between organic matter and persistent contaminants in the suspended matter is vital for ecoefficient estuary recovery.However,little is known regarding aeration effects in suspended particulate aggregates.Therefore,this study aimed to investigate the effects of aeration on the suspended matter from a Tropical and Eutrophic estuarine environment.Anoxic water with 60 g/L of suspended particulate matter(SPM)was collected from Guanabara Bay,Rio de Janeiro,Brazil,transferred to experimental boxes and aerated for 61 days.SPM aggregates monitoring included abiotic variables measurements and,determination of total organic matter(TOM),biopolymers composition,bacterial activity,trace metals,and polycyclic aromatic hydrocarbons(PAHs)concentrations.The aeration enhanced dissolved oxygen(DO)concentration and the redox potential(Eh).However,from days 0 to 61 the predominant bacterial activities were denitrification and fermentation.Electron transport system activity increased after day 10,and aerobic activity was detected after day 19.In summary,aeration increased aerobic bacterial activity,lipids(LIP)and trace metal concentrations,although diminished protein/carbohydrate ratio and PAH concentration.Trace metals concentration(Ni,Pb,Cu,Cr,Mn,and Fe)were the highest on day 19 when the p H was 5.9.Copper presented toxic values(Cu>20.0μg/g).The p H showed a strong negative correlation with Eh(r=-0.94;p<0.001).Acidic environment(p H≤5.9)in marine ecosystems with high loads of toxic trace metals is unsafe for biota.Therefore,managers must be aware of the environmental and biological risks of introducing the aeration technique into a eutrophic marine environment.
