Recently,parasitoid wasp species Microplitis mediator has evoked increasing research attention due to its possible use in the control of Lepidoptera insects.Because insect development involves changes in cuticle compo...Recently,parasitoid wasp species Microplitis mediator has evoked increasing research attention due to its possible use in the control of Lepidoptera insects.Because insect development involves changes in cuticle composition,identification and expression analysis of M.mediator cuticular proteins may clarify the mechanisms involved in parasite development processes.We found 70 cuticular proteins from the M.mediator transcrip-tome and divided them into seven distinct families.Expression profiling indicated that most of these cuticular protein genes have expression peaks specific for one particular developmental stage of M.mediator.Eggs and pupae have the highest number of tran-scriptionally active cuticular protein genes(47 and 52 respectively).Only 12 of these genes maintained high expression activity during late larval development.Functional analysis of two larval proteins,MmCPR3 and MmCPR 14,suggested their important role in the proper organization of the cuticle layers of larvac.During M.mediator larval development,normal cuticle formation can be supported by a limited number of cuticular proteins.展开更多
基金This work was supported by the National Key Plan for Scientific Research and Development of China(2017YFD0200400)and the National Key Plan for Scientific Research and Development of China(2016YFC1200603)+1 种基金the National Natural Science Foun-dation of China(31872298)Open Research Fund Pro-gram of State Key Laboratory ofIPM(Chinese IPM 1803).
文摘Recently,parasitoid wasp species Microplitis mediator has evoked increasing research attention due to its possible use in the control of Lepidoptera insects.Because insect development involves changes in cuticle composition,identification and expression analysis of M.mediator cuticular proteins may clarify the mechanisms involved in parasite development processes.We found 70 cuticular proteins from the M.mediator transcrip-tome and divided them into seven distinct families.Expression profiling indicated that most of these cuticular protein genes have expression peaks specific for one particular developmental stage of M.mediator.Eggs and pupae have the highest number of tran-scriptionally active cuticular protein genes(47 and 52 respectively).Only 12 of these genes maintained high expression activity during late larval development.Functional analysis of two larval proteins,MmCPR3 and MmCPR 14,suggested their important role in the proper organization of the cuticle layers of larvac.During M.mediator larval development,normal cuticle formation can be supported by a limited number of cuticular proteins.
