The development of a cost-competitive bioprocess requires that the cell factory converts the feedstock into the product of interest at high rates and yields.However,microbial cell factories are exposed to a variety of...The development of a cost-competitive bioprocess requires that the cell factory converts the feedstock into the product of interest at high rates and yields.However,microbial cell factories are exposed to a variety of different stresses during the fermentation process.These stresses can be derived from feedstocks,metabolism,or industrial production processes,limiting production capacity and diminishing competitiveness.Improving stress tolerance and robustness allows for more efficient production and ultimately makes a process more economically viable.This review summarises general trends and updates the most recent developments in technologies to improve the stress tolerance of microorganisms.We first look at evolutionary,systems biology and computational methods as examples of non-rational approaches.Then we review the(semi-)rational approaches of membrane and tran-scription factor engineering for improving tolerance phenotypes.We further discuss challenges and perspectives associated with these different approaches.展开更多
基金the Novo Nordisk Foundation(NNF18OC0034844)the Chalmers Foundation and Angpanneforeningens Forskningsstiftelse.
文摘The development of a cost-competitive bioprocess requires that the cell factory converts the feedstock into the product of interest at high rates and yields.However,microbial cell factories are exposed to a variety of different stresses during the fermentation process.These stresses can be derived from feedstocks,metabolism,or industrial production processes,limiting production capacity and diminishing competitiveness.Improving stress tolerance and robustness allows for more efficient production and ultimately makes a process more economically viable.This review summarises general trends and updates the most recent developments in technologies to improve the stress tolerance of microorganisms.We first look at evolutionary,systems biology and computational methods as examples of non-rational approaches.Then we review the(semi-)rational approaches of membrane and tran-scription factor engineering for improving tolerance phenotypes.We further discuss challenges and perspectives associated with these different approaches.
