重症监护病房获得性肌无力(intensive care unit acquired weakness,ICUAW)是重症监护病房患者出现的进行性全身肢体无力,且除危重病本身外无其他原因可解释的一组临床综合征,主要表现为神经肌肉功能障碍。ICUAW的发病机制涉及中枢、周...重症监护病房获得性肌无力(intensive care unit acquired weakness,ICUAW)是重症监护病房患者出现的进行性全身肢体无力,且除危重病本身外无其他原因可解释的一组临床综合征,主要表现为神经肌肉功能障碍。ICUAW的发病机制涉及中枢、周围神经系统和肌纤维内复杂的功能、结构改变,但尚未完全明确。该文综述了目前关于ICUAW的潜在发病机制,以期对ICUAW的诊治提供新思路。展开更多
In this work, the photosynthetic performances of Enteromorpha prolifera thalli collected from the sur- face and bottom of the sea of Qingdao sea area were studied with chlorophyll fluorescence and oxy- graph technolog...In this work, the photosynthetic performances of Enteromorpha prolifera thalli collected from the sur- face and bottom of the sea of Qingdao sea area were studied with chlorophyll fluorescence and oxy- graph technology. The samples with the highest photosynthetic activity among their kinds, the floating thalli from the sea surface of the south of the Qingdao Olympic Sailing Center and the sedimentary thalli from the mud surface of the bottom Tuandao bay, were chosen as representatives of surface thalli and bottom thalli, respectively. The results showed that the maximal PSII quantum yield of the floating thalli was significantly lower than the normal level although their photosynthetic activities were relatively high; the photosynthetic potential of the thalli form the mud surface was extremely low. Thus, it is indicated that the floating thalli are seriously stressed by their environment and the thalli from the mud surface are already dead or are dying. On the other hand, the results of the laboratory cultivation showed that the sedimentary thalli cannot regain normal photosynthetic activity even under normal illumination condi- tions. Thus, the thalli from the mud surface cannot become reproductive source of the alga even if they can reach sea surface again. Therefore, a preliminary conclusion can be reached that, up to mid-July 2008, the environmental conditions of the Qingdao sea area are not suitable for the growth of the alga E. prolifera and for this reason the biomass of E. prolifera, in the area, could be declining.展开更多
Acid, alkali, heat-shock, KNO3 and control pretreatment methods applied to anaerobic sludge were evaluated for their ability to selectively enrich the marine hydrogen-producing mixed microflora. Sea-water culture medi...Acid, alkali, heat-shock, KNO3 and control pretreatment methods applied to anaerobic sludge were evaluated for their ability to selectively enrich the marine hydrogen-producing mixed microflora. Sea-water culture medium was used as the substrate. The hydrogen yield of pretreated microflora was higher than that of the un-pretreated control (P < 0.05). Among the pretreatment methods studied, heat-shock pretreatment yielded the greatest hydrogen production, which was 14.6 times that of the control. When the effect of initial pH on hydrogen production of heat-shock pretreated samples was studied, hydrogen was produced over the entire pH range (pH 4 - 10). The hydrogen yield peaked at initial pH 8 (79 mL/g sucrose) and then steadily decreased as the initial pH increased. Sucrose con-sumption was high at neutral initial pH. During the process of hydrogen production, pH decreased gradually, which indicated that the acquired microflora consisted of acidogenic bacteria.展开更多
Pseudogenes share sequence similarities with functional genes,but in general they have lost their protein-coding ability.The identification of pseudogenes is a very important step in genome annotation.Phaeodactylum tr...Pseudogenes share sequence similarities with functional genes,but in general they have lost their protein-coding ability.The identification of pseudogenes is a very important step in genome annotation.Phaeodactylum tricornutum is a marine diatom that is rich in polyunsaturated fatty acids(PUFAs).The genome of P.tricornutum has been completely sequenced.To identify pseudogenes in P.tricornutum,we developed a pipeline to discover and characterize pseudogenes.We identified a total of 1654 'true' processed pseudogenes,714 duplicated pseudogenes and 4729 pseudogene fragments.The results of the bioinformatics analysis indicated that the genome sequence of P.tricornutum contained many pseudogenes and pseudogene fragments.展开更多
文摘重症监护病房获得性肌无力(intensive care unit acquired weakness,ICUAW)是重症监护病房患者出现的进行性全身肢体无力,且除危重病本身外无其他原因可解释的一组临床综合征,主要表现为神经肌肉功能障碍。ICUAW的发病机制涉及中枢、周围神经系统和肌纤维内复杂的功能、结构改变,但尚未完全明确。该文综述了目前关于ICUAW的潜在发病机制,以期对ICUAW的诊治提供新思路。
基金Supported by National Natural Science Foundation of China (Grant No. 30830015)Key Projects in the National Science & Technology Pillar Program in the Eleventh Five-year Plan Period (Grant No. 2006BAD09A04)National High Technology Research and Development Program of China (Grant Nos. 2006AA10A402, 2007AA09Z406, 2006AA05Z112, 2006AA10A413)
文摘In this work, the photosynthetic performances of Enteromorpha prolifera thalli collected from the sur- face and bottom of the sea of Qingdao sea area were studied with chlorophyll fluorescence and oxy- graph technology. The samples with the highest photosynthetic activity among their kinds, the floating thalli from the sea surface of the south of the Qingdao Olympic Sailing Center and the sedimentary thalli from the mud surface of the bottom Tuandao bay, were chosen as representatives of surface thalli and bottom thalli, respectively. The results showed that the maximal PSII quantum yield of the floating thalli was significantly lower than the normal level although their photosynthetic activities were relatively high; the photosynthetic potential of the thalli form the mud surface was extremely low. Thus, it is indicated that the floating thalli are seriously stressed by their environment and the thalli from the mud surface are already dead or are dying. On the other hand, the results of the laboratory cultivation showed that the sedimentary thalli cannot regain normal photosynthetic activity even under normal illumination condi- tions. Thus, the thalli from the mud surface cannot become reproductive source of the alga even if they can reach sea surface again. Therefore, a preliminary conclusion can be reached that, up to mid-July 2008, the environmental conditions of the Qingdao sea area are not suitable for the growth of the alga E. prolifera and for this reason the biomass of E. prolifera, in the area, could be declining.
基金Supported by the National High Technology Research and Development Program of China (Grant No. 2006AA05Z112)
文摘Acid, alkali, heat-shock, KNO3 and control pretreatment methods applied to anaerobic sludge were evaluated for their ability to selectively enrich the marine hydrogen-producing mixed microflora. Sea-water culture medium was used as the substrate. The hydrogen yield of pretreated microflora was higher than that of the un-pretreated control (P < 0.05). Among the pretreatment methods studied, heat-shock pretreatment yielded the greatest hydrogen production, which was 14.6 times that of the control. When the effect of initial pH on hydrogen production of heat-shock pretreated samples was studied, hydrogen was produced over the entire pH range (pH 4 - 10). The hydrogen yield peaked at initial pH 8 (79 mL/g sucrose) and then steadily decreased as the initial pH increased. Sucrose con-sumption was high at neutral initial pH. During the process of hydrogen production, pH decreased gradually, which indicated that the acquired microflora consisted of acidogenic bacteria.
基金supported by the National Basic Projects for Science and Technology (SQ2012FY4910019-1)the Nonprofit Research Project for the State Oceanic Administration (201105008-2)the National Natural Science Foundation of China (41176137)
文摘Pseudogenes share sequence similarities with functional genes,but in general they have lost their protein-coding ability.The identification of pseudogenes is a very important step in genome annotation.Phaeodactylum tricornutum is a marine diatom that is rich in polyunsaturated fatty acids(PUFAs).The genome of P.tricornutum has been completely sequenced.To identify pseudogenes in P.tricornutum,we developed a pipeline to discover and characterize pseudogenes.We identified a total of 1654 'true' processed pseudogenes,714 duplicated pseudogenes and 4729 pseudogene fragments.The results of the bioinformatics analysis indicated that the genome sequence of P.tricornutum contained many pseudogenes and pseudogene fragments.
