Faulkner's masterpiece The Sound and The Fury helps him win world reputation.He uses the creative writing method by combining the stream of consciousness and traditional writing ways.This paper explores the creati...Faulkner's masterpiece The Sound and The Fury helps him win world reputation.He uses the creative writing method by combining the stream of consciousness and traditional writing ways.This paper explores the creativity in his writing method through analysis of the four sections of the novel.展开更多
Background Resistin, a newly discovered cysteine-rich hormone secreted mainly by adipose tissues, has been proposed to form a biochemical link between obesity and type 2 diabetes. However, the resistin receptor has no...Background Resistin, a newly discovered cysteine-rich hormone secreted mainly by adipose tissues, has been proposed to form a biochemical link between obesity and type 2 diabetes. However, the resistin receptor has not yet been identified. This study aimed to identify resistin binding proteins/receptor. Methods Three cDNA fragments with the same 11 bp 5＇ sequence were found by screening a cDNA phage display library of rat multiple tissues. As the reading frames of the same 11 bp 5＇ sequence were interrupted by a TGA stop codon, plaque lift assay was consequently used to prove the readthrough phenomenon. The stop codon in the same 11 bp 5＇ sequence was replaced by tryptophan, and the binding activity of the coded peptide [AWIL, which was designated as resistin binding peptide （RBP）] with resistin was identified by the confocal microscopy technique and the affinity chromatography experiment, pDual GC-resistin and pDual GC-resistin binding peptide were co-transfected into 3T3-L1 cells to confirm the function of resistin binding peptide. Results Three cDNA fragments with the same 11 bp 5＇ sequence were found. The TGA stop codon in reading frames of the same 11 bp 5＇ sequence was proved to be readthroughed. The binding activity of RBP with resistin was consequently identified. The expression of the resistin binding peptide in 3T3-L1 preadipocytes expressing pDual G-C-resistin significantly inhibited the adipogenic differentiation. Conclusion RBP could effectively rescue the promoted differentiation of resistin overxepressed 3T3-L1 preadipocyte.展开更多
文摘面向目标监测任务的无线传感网(Wireless Sensor Network,WSN)通常部署在无人监管和关键敏感的环境中,无线通信的开放性严重威胁了监测目标的安全性,因此需要对源节点位置隐私进行有效保护。针对现有WSN源位置隐私保护方案普遍存在的高延迟和高能耗问题,提出了一种基于改进蚁群算法的源位置隐私保护方案EESLP-ACA(Energy Efficient Source Location Privacy based on Ant Colony Algorithm)。传感器节点接收到数据包时,将根据信息素浓度和改进的路径启发素含量选择转发节点,以最小化和均衡化网络能耗;同时通过引入参照距离并改进信息素更新机制,增大未选中节点成为转发节点的可能性,构建低概率重复动态路由,减少攻击者能够接收到的数据包数目,增加反向追踪的难度。性能分析表明,所提方案不但能有效提高蚁群算法(Ant Colony Algorithm,ACA)的性能,使其更好地应用于WSN源位置隐私保护领域;而且相较于CDR和ELSP方案,在延长网络生存周期和缩短传输延迟的同时,能有效提升源位置隐私的安全性。
文摘Faulkner's masterpiece The Sound and The Fury helps him win world reputation.He uses the creative writing method by combining the stream of consciousness and traditional writing ways.This paper explores the creativity in his writing method through analysis of the four sections of the novel.
基金This study was supported by grants from the National Natural Science Foundation of China (No. 30371502) the Natural Science Foundation of Jiangsu Province (No. BK2001120)Jiangsu Provincial Health Bureau (No. RC2002061).
文摘Background Resistin, a newly discovered cysteine-rich hormone secreted mainly by adipose tissues, has been proposed to form a biochemical link between obesity and type 2 diabetes. However, the resistin receptor has not yet been identified. This study aimed to identify resistin binding proteins/receptor. Methods Three cDNA fragments with the same 11 bp 5＇ sequence were found by screening a cDNA phage display library of rat multiple tissues. As the reading frames of the same 11 bp 5＇ sequence were interrupted by a TGA stop codon, plaque lift assay was consequently used to prove the readthrough phenomenon. The stop codon in the same 11 bp 5＇ sequence was replaced by tryptophan, and the binding activity of the coded peptide [AWIL, which was designated as resistin binding peptide （RBP）] with resistin was identified by the confocal microscopy technique and the affinity chromatography experiment, pDual GC-resistin and pDual GC-resistin binding peptide were co-transfected into 3T3-L1 cells to confirm the function of resistin binding peptide. Results Three cDNA fragments with the same 11 bp 5＇ sequence were found. The TGA stop codon in reading frames of the same 11 bp 5＇ sequence was proved to be readthroughed. The binding activity of RBP with resistin was consequently identified. The expression of the resistin binding peptide in 3T3-L1 preadipocytes expressing pDual G-C-resistin significantly inhibited the adipogenic differentiation. Conclusion RBP could effectively rescue the promoted differentiation of resistin overxepressed 3T3-L1 preadipocyte.
