Quantum dots have been widely applied in biosensing due to their outstanding optical properties.The emissions of quantum dots are mainly determined by their composition and size,as described by the Brus’s equation.So...Quantum dots have been widely applied in biosensing due to their outstanding optical properties.The emissions of quantum dots are mainly determined by their composition and size,as described by the Brus’s equation.Somehow,in this case,their emissions are hardly regulated reversibly and responsively,which are unsuitable for biosensing and biodetection.In the last decade,capping ligands have been used for designing biosensors because of their responsive regulation on the photoluminescence of quantum dots.Here,we first summarize the advances in characterization and calculation specific for ligands,which have helped to provide insights into the photoluminescence process and energy band theory of quantum dots.We then review two ways of ligand design that influence the optical properties of quantum dots:affecting the process of photoluminescence,or the orbital/electronic structure.In the latter case,the atoms on both the ligand and the surface of the quantum dot interact to affect the energy band structure of the quantum dot core.Examples are presented of how these quantum dots that possess responsive properties due to the design of the ligands have been applied to sensing.With further exploration,we hope to see advances in the fundamental understanding of the energy band structures and practical applications of these quantum dots.展开更多
The history, characteristic, operation modes and coupling techniques of atomic force microscopy (AFM) are introduced. Then the application in cell biology is re- viewed in four aspects: cell immobilization methods, ce...The history, characteristic, operation modes and coupling techniques of atomic force microscopy (AFM) are introduced. Then the application in cell biology is re- viewed in four aspects: cell immobilization methods, cell im- aging, force spectrum study and cell manipulation. And the prospect of AFM application in cell biology is discussed.展开更多
Microarray technology has been proved to be greatly helpful for biomedical and biological diagnosis. And the evaluation of its biological applications lies in the detection sensitivity, which requires high intensity a...Microarray technology has been proved to be greatly helpful for biomedical and biological diagnosis. And the evaluation of its biological applications lies in the detection sensitivity, which requires high intensity and stability of the signal. Recently, several nanomaterials, especially semiconductor nanomaterials, due to their excellent fluorescence properties, have been widely used to construct microarrays for biosensors. Here, we presented an approach for constructing CdSe/ZnS quantum dot (QD) microarray in microfluidic channels on a glass slide by photolithography. The conditions for immobilizing stable and uniform QD microarray on the glass slide were optimized. Several types of QD microarrays with different emission wavelengths and modified groups were constructed using silanization and lithography technology. Based on the fluorescence quenching effect of Cu2+ on QDs, the microfluidic chip with QD microarray was applied for the determination of Cu2+. 1 nmol/L Cu2+ could be detected by this method.展开更多
基金supported by the National Natural Science Foundation of China(Nos.22293030 and 22293032)the National Key Research and Development Program of China(Nos.2019YFA0210100 and 2023YFB3208301)the Fundamental Research Funds for the Central Universities of China(No.63211023).
文摘Quantum dots have been widely applied in biosensing due to their outstanding optical properties.The emissions of quantum dots are mainly determined by their composition and size,as described by the Brus’s equation.Somehow,in this case,their emissions are hardly regulated reversibly and responsively,which are unsuitable for biosensing and biodetection.In the last decade,capping ligands have been used for designing biosensors because of their responsive regulation on the photoluminescence of quantum dots.Here,we first summarize the advances in characterization and calculation specific for ligands,which have helped to provide insights into the photoluminescence process and energy band theory of quantum dots.We then review two ways of ligand design that influence the optical properties of quantum dots:affecting the process of photoluminescence,or the orbital/electronic structure.In the latter case,the atoms on both the ligand and the surface of the quantum dot interact to affect the energy band structure of the quantum dot core.Examples are presented of how these quantum dots that possess responsive properties due to the design of the ligands have been applied to sensing.With further exploration,we hope to see advances in the fundamental understanding of the energy band structures and practical applications of these quantum dots.
基金supported by the National Natural Science Foundation of China(Grant No.20305011)the Nationa1 Science Fund for Distinguished Young Scholars(Grant No.20025311).
文摘The history, characteristic, operation modes and coupling techniques of atomic force microscopy (AFM) are introduced. Then the application in cell biology is re- viewed in four aspects: cell immobilization methods, cell im- aging, force spectrum study and cell manipulation. And the prospect of AFM application in cell biology is discussed.
基金supported by the National Basic Research Program of China (2011CB933600)the Science Fund for Creative Research Groups (20921062)+1 种基金the National Natural Science Foundation of China (21175100)the Program for New Century Excellent Talents in University (NCET-10-0656)
文摘Microarray technology has been proved to be greatly helpful for biomedical and biological diagnosis. And the evaluation of its biological applications lies in the detection sensitivity, which requires high intensity and stability of the signal. Recently, several nanomaterials, especially semiconductor nanomaterials, due to their excellent fluorescence properties, have been widely used to construct microarrays for biosensors. Here, we presented an approach for constructing CdSe/ZnS quantum dot (QD) microarray in microfluidic channels on a glass slide by photolithography. The conditions for immobilizing stable and uniform QD microarray on the glass slide were optimized. Several types of QD microarrays with different emission wavelengths and modified groups were constructed using silanization and lithography technology. Based on the fluorescence quenching effect of Cu2+ on QDs, the microfluidic chip with QD microarray was applied for the determination of Cu2+. 1 nmol/L Cu2+ could be detected by this method.
