Drought is one of the most adverse environmental factors that impact on plant growth and reduce crop yields. To decipher the molecular mechanism of drought tolerance, we constructed a cDNA library using suppression su...Drought is one of the most adverse environmental factors that impact on plant growth and reduce crop yields. To decipher the molecular mechanism of drought tolerance, we constructed a cDNA library using suppression subtractive hybridization （SSH） and dissected the gene expression profiles in seedling and jointing wheat plants after stress with PEG-6000. A total of 2 046 ESTs from the jointing stage library （J-Lib） were allocated to 961 contigs. Among the ESTs, 265 uni-genes in 12 categories were identified on the basis of sequence similarities and functional classifications. Most were known to be involved in protection, directly or indirectly, against water stress. To determine differences in gene expression profiles for water stress responses at the jointing and seedling stages, data from the J-Lib were compared with those from a 2- leaf seedling library （S-Lib） constructed by the same method. Significant differences were observed between the two libraries for function-known genes; signal transduction genes were far more active at jointing than at the seedling stage.展开更多
The present study was designed to investigate the antithrombotic effects and underlying mechanisms of the effective components group(ECG) of Xiaoshuantongluo recipe(XECG) and to further verify the rationality and feas...The present study was designed to investigate the antithrombotic effects and underlying mechanisms of the effective components group(ECG) of Xiaoshuantongluo recipe(XECG) and to further verify the rationality and feasibility of ECG-guided methodology in traditional Chinese medicine(TCM) research. The arterial thrombosis model induced by ferric chloride(FeC l3) oxidation and the venous thrombosis model induced by inferior vena cava ligation were established to evaluate the antithrombotic potential of XECG. Our results indicated that XECG significantly prolonged the time to occlusion, activated partial thromboplastin time(APTT), and prothrombin time(PT), and markedly inhibited adenosine diphosphate(ADP)-induced platelet aggregation in the 20% Fe Cl3-induced arterial thrombosis model. The superoxide dismutase(SOD) activity was significantly increased and the levels of malondialdehyde(MDA) and nitric oxide(NO) were dramatically decreased in the plasma of arterial thrombosis rats after XECG treatment for 12 days. Furthermore, XECG markedly reduced the weight of thrombus formed by inferior vena cava ligation. Additionally, XECG exhibited 2,2-diphenyl-1-picrylhydrazyl(DPPH) free radical scavenging activity and protective effect on mitochondrial lipid peroxidation. In summary, XECG played an important role in the prevention of thrombosis through interacting with multiple targets, including inhibition of platelet aggregation and coagulation and repression of oxidative stress. The ECG-guided methodology was validated as a feasible tool in TCM research.展开更多
基金supported by the National High-TechR&D Program of China (863 Program, 2006AA100201)the National Transgenic Plants Program of China(2008ZX08002-002)
文摘Drought is one of the most adverse environmental factors that impact on plant growth and reduce crop yields. To decipher the molecular mechanism of drought tolerance, we constructed a cDNA library using suppression subtractive hybridization （SSH） and dissected the gene expression profiles in seedling and jointing wheat plants after stress with PEG-6000. A total of 2 046 ESTs from the jointing stage library （J-Lib） were allocated to 961 contigs. Among the ESTs, 265 uni-genes in 12 categories were identified on the basis of sequence similarities and functional classifications. Most were known to be involved in protection, directly or indirectly, against water stress. To determine differences in gene expression profiles for water stress responses at the jointing and seedling stages, data from the J-Lib were compared with those from a 2- leaf seedling library （S-Lib） constructed by the same method. Significant differences were observed between the two libraries for function-known genes; signal transduction genes were far more active at jointing than at the seedling stage.
基金supported by the Major Scientific and Technological Special Project for"Significant New Drugs Creation"(No.2013ZX09508104001002)the National Natural Science Foundation of China(No.81273652)
文摘The present study was designed to investigate the antithrombotic effects and underlying mechanisms of the effective components group(ECG) of Xiaoshuantongluo recipe(XECG) and to further verify the rationality and feasibility of ECG-guided methodology in traditional Chinese medicine(TCM) research. The arterial thrombosis model induced by ferric chloride(FeC l3) oxidation and the venous thrombosis model induced by inferior vena cava ligation were established to evaluate the antithrombotic potential of XECG. Our results indicated that XECG significantly prolonged the time to occlusion, activated partial thromboplastin time(APTT), and prothrombin time(PT), and markedly inhibited adenosine diphosphate(ADP)-induced platelet aggregation in the 20% Fe Cl3-induced arterial thrombosis model. The superoxide dismutase(SOD) activity was significantly increased and the levels of malondialdehyde(MDA) and nitric oxide(NO) were dramatically decreased in the plasma of arterial thrombosis rats after XECG treatment for 12 days. Furthermore, XECG markedly reduced the weight of thrombus formed by inferior vena cava ligation. Additionally, XECG exhibited 2,2-diphenyl-1-picrylhydrazyl(DPPH) free radical scavenging activity and protective effect on mitochondrial lipid peroxidation. In summary, XECG played an important role in the prevention of thrombosis through interacting with multiple targets, including inhibition of platelet aggregation and coagulation and repression of oxidative stress. The ECG-guided methodology was validated as a feasible tool in TCM research.
