The present paper describes multiple splicing types of OsRIX4,an RAD21 homolog in rice. A type of alternative splicing (AS),distinctive from all five previously known splicing types,was identified in which interior se...The present paper describes multiple splicing types of OsRIX4,an RAD21 homolog in rice. A type of alternative splicing (AS),distinctive from all five previously known splicing types,was identified in which interior sequences of a constitutive exon could be spliced. Translation of the transcript produced with this AS type was demonstrated at the protein level. Expression of multiple transcripts was organ specific. The expression abundance of transcripts,OsRIX4-4 and OsRIX4-5,was positively correlated with fertility in rice. The splicing type identified in the present study provided the means to further un-derstand and define different mRNA splicing types in plants and suggested that post-transcription processing of mRNA and its regulation mechanism are complex.展开更多
The T gene, which was cloned from the mitochondria of tumorous stem mustard （Brassica juncea var. tumida）, is a cytoplas- mic male sterility （CMS）-related gene that can produce two transcripts, T1170 and T1243. Th...The T gene, which was cloned from the mitochondria of tumorous stem mustard （Brassica juncea var. tumida）, is a cytoplas- mic male sterility （CMS）-related gene that can produce two transcripts, T1170 and T1243. The latter is transcribed with the un- cleaved intron Tinll. In our previous study, transgenic Arabidopsis thaliana plants over-expressing the T1243 transcript （OE-T1243） showed a severe male-sterile phenotype, whereas OE-Tll70 plants did not. However, the functional mechanism of the T gene in B. Juncea remained unknown. In this study, microscopic analyses of paraffin sections of anthers confirmed that OE-T1243 plants did not produce normal pollen, whereas OE-T1170 plants did. We analyzed the transcription of 15 anther development-related genes and found that transcript levels of nozzle/sporocyteless and barely any meristem 1 and 2 were markedly lower in OE-T1243 plants than those in wild type, while the transcript levels of these genes in OE-Tll70 plants were unchanged. To investigate the potential roles of TinH, we inserted the TinH sequence upstream of a minimal region （-60） of the cauliflower mosaic virus 35S promoter fused to the 5＇ end of the 13-glucuronidase （GUS） reporter gene. Analysis of the transgenic plants suggested that TinH acted as an enhancer to significantly increase GUS expression. The potential action mechanism is that the TinH intron acts as an enhancer to affect the function of the CMS-related gene T.展开更多
基金Supported by the National Natural Science Foundation of China (Grant No. 30170592)Zhejiang Provincial Science and Technology Project (Grant No. 2005C22002)
文摘The present paper describes multiple splicing types of OsRIX4,an RAD21 homolog in rice. A type of alternative splicing (AS),distinctive from all five previously known splicing types,was identified in which interior sequences of a constitutive exon could be spliced. Translation of the transcript produced with this AS type was demonstrated at the protein level. Expression of multiple transcripts was organ specific. The expression abundance of transcripts,OsRIX4-4 and OsRIX4-5,was positively correlated with fertility in rice. The splicing type identified in the present study provided the means to further un-derstand and define different mRNA splicing types in plants and suggested that post-transcription processing of mRNA and its regulation mechanism are complex.
基金supported by the National Natural Science Foundation ofChina(31071809)to Pei YanXi
文摘The T gene, which was cloned from the mitochondria of tumorous stem mustard （Brassica juncea var. tumida）, is a cytoplas- mic male sterility （CMS）-related gene that can produce two transcripts, T1170 and T1243. The latter is transcribed with the un- cleaved intron Tinll. In our previous study, transgenic Arabidopsis thaliana plants over-expressing the T1243 transcript （OE-T1243） showed a severe male-sterile phenotype, whereas OE-Tll70 plants did not. However, the functional mechanism of the T gene in B. Juncea remained unknown. In this study, microscopic analyses of paraffin sections of anthers confirmed that OE-T1243 plants did not produce normal pollen, whereas OE-T1170 plants did. We analyzed the transcription of 15 anther development-related genes and found that transcript levels of nozzle/sporocyteless and barely any meristem 1 and 2 were markedly lower in OE-T1243 plants than those in wild type, while the transcript levels of these genes in OE-Tll70 plants were unchanged. To investigate the potential roles of TinH, we inserted the TinH sequence upstream of a minimal region （-60） of the cauliflower mosaic virus 35S promoter fused to the 5＇ end of the 13-glucuronidase （GUS） reporter gene. Analysis of the transgenic plants suggested that TinH acted as an enhancer to significantly increase GUS expression. The potential action mechanism is that the TinH intron acts as an enhancer to affect the function of the CMS-related gene T.
