目的建立以反相高效液相色谱(RP-HPLC)法测定颈痛消丸中龙胆苦苷含量的方法。方法采用Diamonsic C 18(4.6mm×150mm,5μm)色谱柱;使用甲醇-水(28∶72)为流动相进行等度洗脱;流速为1.0mL·min^-1;柱温为30℃;检测波长为277nm。...目的建立以反相高效液相色谱(RP-HPLC)法测定颈痛消丸中龙胆苦苷含量的方法。方法采用Diamonsic C 18(4.6mm×150mm,5μm)色谱柱;使用甲醇-水(28∶72)为流动相进行等度洗脱;流速为1.0mL·min^-1;柱温为30℃;检测波长为277nm。结果龙胆苦苷进样量在0.185~1.297μg范围内与峰面积积分值呈良好线性关系(r=0.9998);平均回收率为98.71%,RSD为1.10%。结论该方法简便准确、专属性强、重复性好,可用于颈痛消丸的质量控制。展开更多
As the development of the global communication all over the world, English has become the international language for the global communication. Furthermore, speaking as a skill in English language learning becomes more...As the development of the global communication all over the world, English has become the international language for the global communication. Furthermore, speaking as a skill in English language learning becomes more and more important. As it is known, spoken English has specific features, thus to gain an explicit understanding of the features data analysis is helpful. Moreover it is useful for language teacher to make development in teaching to satisfy learner's needs. Grammatical and phonological are the two remarkable aspects and specific features in spoken language therefore to discover elements into these aspects seems helpful to teaching spoken Enlgish.展开更多
文摘目的建立以反相高效液相色谱(RP-HPLC)法测定颈痛消丸中龙胆苦苷含量的方法。方法采用Diamonsic C 18(4.6mm×150mm,5μm)色谱柱;使用甲醇-水(28∶72)为流动相进行等度洗脱;流速为1.0mL·min^-1;柱温为30℃;检测波长为277nm。结果龙胆苦苷进样量在0.185~1.297μg范围内与峰面积积分值呈良好线性关系(r=0.9998);平均回收率为98.71%,RSD为1.10%。结论该方法简便准确、专属性强、重复性好,可用于颈痛消丸的质量控制。
文摘As the development of the global communication all over the world, English has become the international language for the global communication. Furthermore, speaking as a skill in English language learning becomes more and more important. As it is known, spoken English has specific features, thus to gain an explicit understanding of the features data analysis is helpful. Moreover it is useful for language teacher to make development in teaching to satisfy learner's needs. Grammatical and phonological are the two remarkable aspects and specific features in spoken language therefore to discover elements into these aspects seems helpful to teaching spoken Enlgish.
