Objective To investigate the therapeutic potential of tumor necrosis factor （TNF）-related apoptosis-inducing ligand （TRAIL）, a member of the TNF superfamily, and to analyze TRAIL-induced apoptosis in Jurkat cells....Objective To investigate the therapeutic potential of tumor necrosis factor （TNF）-related apoptosis-inducing ligand （TRAIL）, a member of the TNF superfamily, and to analyze TRAIL-induced apoptosis in Jurkat cells. Methods Expression of TRAIL receptors （DR4 and DR5） was detected by reverse transcriptase-polymerase chain reaction （RT-PCR）. Cytotoxic effects were determined by colony formation assay and a cell counting kit. The effects of recombinant TRAIL on apoptosis of Jurkat cells were determined by DNA fragmentation （DNA ladder） and PI staining. Changes in mitochondrial membrane potential were detected with JC-1 fluorescence. Results TRAIL inhibited the proliferation and induced intemucleosomal DNA fragmentation （characteristic of apoptosis） and loss of mitochondrial membrane potential. Conclusion Recombinant soluble TRAIL can be used as a therapy for cancer.展开更多
The design of exhaust hood is a typical high dimensional,expensive computational and black box problem.Multi-Point Search based Efficient Global Optimization(MSEGO)is proposed to solve this problem.MSEGO is used for t...The design of exhaust hood is a typical high dimensional,expensive computational and black box problem.Multi-Point Search based Efficient Global Optimization(MSEGO)is proposed to solve this problem.MSEGO is used for the aerodynamic performance optimization of a low exhaust hood with non-axisymmetric outer flow guider.After optimization,the static pressure coefficient of the exhaust hood increases by 284.54%,and the aerodynamic performance analysis explains the reason of the improvement.Further,the analysis of variance(ANOVA)as the data mining technique is used to extract information of design space and analyze the influence of variables on the performance.Though aerodynamic performance analysis and data mining,it indicates that non-axisymmetric outer flow guider and the width of outer hood has a significant effect on the aerodynamic performance.Thereby,design lessons are derived and accumulated for the optimization of similar designs.展开更多
基金This research was supported by Jiangsu Projects for Postdoctoral Research Funds (No. 0601025B) National Projects for Postdoctoral Research Funds (No. 20060390940).
文摘Objective To investigate the therapeutic potential of tumor necrosis factor （TNF）-related apoptosis-inducing ligand （TRAIL）, a member of the TNF superfamily, and to analyze TRAIL-induced apoptosis in Jurkat cells. Methods Expression of TRAIL receptors （DR4 and DR5） was detected by reverse transcriptase-polymerase chain reaction （RT-PCR）. Cytotoxic effects were determined by colony formation assay and a cell counting kit. The effects of recombinant TRAIL on apoptosis of Jurkat cells were determined by DNA fragmentation （DNA ladder） and PI staining. Changes in mitochondrial membrane potential were detected with JC-1 fluorescence. Results TRAIL inhibited the proliferation and induced intemucleosomal DNA fragmentation （characteristic of apoptosis） and loss of mitochondrial membrane potential. Conclusion Recombinant soluble TRAIL can be used as a therapy for cancer.
文摘The design of exhaust hood is a typical high dimensional,expensive computational and black box problem.Multi-Point Search based Efficient Global Optimization(MSEGO)is proposed to solve this problem.MSEGO is used for the aerodynamic performance optimization of a low exhaust hood with non-axisymmetric outer flow guider.After optimization,the static pressure coefficient of the exhaust hood increases by 284.54%,and the aerodynamic performance analysis explains the reason of the improvement.Further,the analysis of variance(ANOVA)as the data mining technique is used to extract information of design space and analyze the influence of variables on the performance.Though aerodynamic performance analysis and data mining,it indicates that non-axisymmetric outer flow guider and the width of outer hood has a significant effect on the aerodynamic performance.Thereby,design lessons are derived and accumulated for the optimization of similar designs.
