以大豆(Glycine max (L.) Merrill)黄化苗为材料,通过优化提取线粒体DNA(mtDNA)时差速离心过程中的离心力和离心时间,以及纯化过程中设置不同的蔗糖密度梯度和裂解液浓度,结合高盐法去除蛋白质,改良大豆mtDNA的提取方法。结果表明,该方...以大豆(Glycine max (L.) Merrill)黄化苗为材料,通过优化提取线粒体DNA(mtDNA)时差速离心过程中的离心力和离心时间,以及纯化过程中设置不同的蔗糖密度梯度和裂解液浓度,结合高盐法去除蛋白质,改良大豆mtDNA的提取方法。结果表明,该方法提取的mtDNA浓度和纯度较高,无叶绿体和核基因组DNA的污染,可用于后续大豆线粒体基因组的相关研究。展开更多
Outcrossing rate is an important determinant of cytoplasmic male sterile(CMS)breeding and hybrid seed production for heterosis in soybean.Parental lines with a high outcrossing rate were screened for backcross breedin...Outcrossing rate is an important determinant of cytoplasmic male sterile(CMS)breeding and hybrid seed production for heterosis in soybean.Parental lines with a high outcrossing rate were screened for backcross breeding to obtain the high outcrossing rate maintenance B-lines and sterile A-lines.Application in production practices will help to increase hybrid soybean production.In this study,JLCMS82B and JLCMS89B were selected as parents for the construction of outcrossing rate segregation populations,and the progeny-array approach(PAA)and glyphosate resistant gene markers were used to determine outcrossing rates.We found that:(1)The outcrossing rate between JLCMS82B and JLCMS89B was significantly different;(2)the outcrossing rate of the F2 segregating populations was a quantitative trait,though whether an additive or epistatic effect exists required analysis with a triple test intersection analysis;(3)agronomic traits correlated with outcrossing rate;outcrossing rate was the highest with plant height of about 84 cm,lower number of plant branches,earlier flowering time,larger angle between the branches and the main stem,and with more divergent plant morphology.Correlation analysis between agronomic traits and outcrossing rate can effectively guide the screening of parents with a high outcrossing rate.展开更多
Significant progress has been achieved in the use of heterosis in soybean and several soybean hybrids have been released in China. However, broad use of hybrid soybean seed is limited due to low seed setting of female...Significant progress has been achieved in the use of heterosis in soybean and several soybean hybrids have been released in China. However, broad use of hybrid soybean seed is limited due to low seed setting of female parents. Breeding cytoplasmic male sterile（CMS） lines with high out-crossing rate is necessary to solve the problem. The objective of this study was to determine the relationship between out-crossing rate of CMS lines and their nectar secretion. The daily nectar secretion rhythm, meteorological effect on nectar secretion, and differences in nectar secretion among genotypes and years were investigated in 27 soybean CMS lines（A-lines） with their maintainers（B-lines） and restorers（R-lines）. The correlation between out-crossing rate of CMS lines and nectar production was also evaluated. Nectar secretion had diurnal variation. Secretion initiated at about 06：00 for most materials and reached a peak at 07：00–08：30 after flower opened, then the nectar secretion decreased gradually. A sub-peak appeared at about 13：00, while the nectar could not be detected at 17：00. Nectar secretion was greatly influenced by the weather conditions. The amount of nectar secretion increased gradually over time during periods of high temperature and no rainfall for several days. Rainy weather and low temperatures inhibited nectar secretion. There were obvious variations of nectar amount among different genotypes tested. Significant nectar variation within a genotype among years was also observed, and the highest nectar secretion was 3-fold higher than the lowest. The amount of nectar secretion from R-lines was significantly higher than that of A-and B-lines. There was no significant difference in nectar secretion between A-and B-lines. A-and B-lines with higher out-crossing rates secreted more nectar. The amount of nectar secretion of A-and B-lines were significantly positively correlated with the out-crossing rate of A-lines.展开更多
There are a large number of historic buildings which were mainly made of blue-brick masonry in today’s world.However,for the natural and man-made reasons,these historic buildings have been damaged in different degree...There are a large number of historic buildings which were mainly made of blue-brick masonry in today’s world.However,for the natural and man-made reasons,these historic buildings have been damaged in different degrees.In order to protect historic buildings more scientifically and learn about the preservation state of existing historic buildings,it is necessary to ascertain the material properties of blue brick in historic buildings.The article takes the blue bricks of historical buildings in Kaifeng area of the Central Plains as an example to study.Through the analysis of physical properties,X-ray fluorescence spectroscopy,X-ray diffraction and scanning electron microscopy of blue brick specimens,the physical properties such as the apparent density,moisture content,porosity,and material structure composition are understood.The results show that the apparent density of blue brick is 1.64 g/cm^(3),the moisture content is 10.23%,the 24 h atmospheric water absorption is 17.86%,and the porosity is 20.99%.The smaller the apparent density is,the larger the porosity is,and the water absorption performance is better.From the microscopic point of view,bonding ability between blue brick mineral particles is relatively weak.The pores between skeletons are large and the pore structure is obvious.From the perspective of material phase,the elements of blue brick are mainly O,Si,Al,Fe,and the composition of blue brick is mainly composed of quartz and feldspar.The softening coefficient of blue brick is 0.80,and the deformation and stability of the structure should be paid special attention in the rainy season or wet environment.Through the frost test,there are salt substances in the internal pores of the brick,and the surface of the blue brick is eroded and pulverized.In this paper,the experimental process and analysis methods for testing the material properties of blue brick can provide reference for the research on the material properties of the same kind of blue-brick masonry in historic buildings and masonry relics.The relevant material property parameters obtained in this paper can provide guidance for making protection schemes and scientific repairs for historic buildings in Central China,enrich the evaluation criteria for maintaining and reinforcing historic buildings,and provide theoretical support for studying the damage and health detection technology related to historic buildings.展开更多
The concept of“New Engineering”has put forward new challenges to the talents cultivation of universities.Due to some problems of the traditional Software Engineering curriculum,e.g.separated design at undergraduate-...The concept of“New Engineering”has put forward new challenges to the talents cultivation of universities.Due to some problems of the traditional Software Engineering curriculum,e.g.separated design at undergraduate-level and graduate-level courses,poor curriculum structure,lacking of industry characteristics.This paper proposes an integrated undergraduate-graduate education curriculum for Software Engineering Major,which is based on Software Engineering specialty knowledge system(C-SWEBOK)and focuses on the current national strategic demands.Additionally,the curriculum combines with the University’s transportation characteristics,and fuses the discipline of Software Engineering and Intelligent Transportation.The multi-level curriculum designed in this paper is with reasonable structure,complete system,progressive content,and salient feature,which provides the strong support for cultivating high-qualified software talents in line with national strategies and industry needs.展开更多
文摘以大豆(Glycine max (L.) Merrill)黄化苗为材料,通过优化提取线粒体DNA(mtDNA)时差速离心过程中的离心力和离心时间,以及纯化过程中设置不同的蔗糖密度梯度和裂解液浓度,结合高盐法去除蛋白质,改良大豆mtDNA的提取方法。结果表明,该方法提取的mtDNA浓度和纯度较高,无叶绿体和核基因组DNA的污染,可用于后续大豆线粒体基因组的相关研究。
基金supported by the National Natural Science Foundation for Young Scientists of China (31301399)the Major Project for Science and Technology Development of Jilin Province, China (20170201001NY)the Agricultural Science Technology Innovation Project of Jilin Province, China (CXGC2017TD002)
文摘Outcrossing rate is an important determinant of cytoplasmic male sterile(CMS)breeding and hybrid seed production for heterosis in soybean.Parental lines with a high outcrossing rate were screened for backcross breeding to obtain the high outcrossing rate maintenance B-lines and sterile A-lines.Application in production practices will help to increase hybrid soybean production.In this study,JLCMS82B and JLCMS89B were selected as parents for the construction of outcrossing rate segregation populations,and the progeny-array approach(PAA)and glyphosate resistant gene markers were used to determine outcrossing rates.We found that:(1)The outcrossing rate between JLCMS82B and JLCMS89B was significantly different;(2)the outcrossing rate of the F2 segregating populations was a quantitative trait,though whether an additive or epistatic effect exists required analysis with a triple test intersection analysis;(3)agronomic traits correlated with outcrossing rate;outcrossing rate was the highest with plant height of about 84 cm,lower number of plant branches,earlier flowering time,larger angle between the branches and the main stem,and with more divergent plant morphology.Correlation analysis between agronomic traits and outcrossing rate can effectively guide the screening of parents with a high outcrossing rate.
基金Supported by the National High Technology Research and Development Programme of China （ No. 2004AA001210） and the National Natural Science Foundation of China （No. 60532030）.
基金supported by the National Key Research and Development Program of China (2016YFD0101500, 2016YFD0101502)the Agricultural Science and Technology Innovation Project of Jilin Province, China (CXGC2017Z004)
文摘Significant progress has been achieved in the use of heterosis in soybean and several soybean hybrids have been released in China. However, broad use of hybrid soybean seed is limited due to low seed setting of female parents. Breeding cytoplasmic male sterile（CMS） lines with high out-crossing rate is necessary to solve the problem. The objective of this study was to determine the relationship between out-crossing rate of CMS lines and their nectar secretion. The daily nectar secretion rhythm, meteorological effect on nectar secretion, and differences in nectar secretion among genotypes and years were investigated in 27 soybean CMS lines（A-lines） with their maintainers（B-lines） and restorers（R-lines）. The correlation between out-crossing rate of CMS lines and nectar production was also evaluated. Nectar secretion had diurnal variation. Secretion initiated at about 06：00 for most materials and reached a peak at 07：00–08：30 after flower opened, then the nectar secretion decreased gradually. A sub-peak appeared at about 13：00, while the nectar could not be detected at 17：00. Nectar secretion was greatly influenced by the weather conditions. The amount of nectar secretion increased gradually over time during periods of high temperature and no rainfall for several days. Rainy weather and low temperatures inhibited nectar secretion. There were obvious variations of nectar amount among different genotypes tested. Significant nectar variation within a genotype among years was also observed, and the highest nectar secretion was 3-fold higher than the lowest. The amount of nectar secretion from R-lines was significantly higher than that of A-and B-lines. There was no significant difference in nectar secretion between A-and B-lines. A-and B-lines with higher out-crossing rates secreted more nectar. The amount of nectar secretion of A-and B-lines were significantly positively correlated with the out-crossing rate of A-lines.
基金The authors would like to express heartfelt gratitude to the financial support by the Science Technology of the Ministry of Housing and Urban-Rural Development(No.2018-K9-065)China Postdoctoral Science Foundation Funded Project(No.2018M632805)+1 种基金Key Scientific and Technological Project of Henan Province(No.212102310932)Key Scientific and Technological Project of Kaifeng City(No.2001010).
文摘There are a large number of historic buildings which were mainly made of blue-brick masonry in today’s world.However,for the natural and man-made reasons,these historic buildings have been damaged in different degrees.In order to protect historic buildings more scientifically and learn about the preservation state of existing historic buildings,it is necessary to ascertain the material properties of blue brick in historic buildings.The article takes the blue bricks of historical buildings in Kaifeng area of the Central Plains as an example to study.Through the analysis of physical properties,X-ray fluorescence spectroscopy,X-ray diffraction and scanning electron microscopy of blue brick specimens,the physical properties such as the apparent density,moisture content,porosity,and material structure composition are understood.The results show that the apparent density of blue brick is 1.64 g/cm^(3),the moisture content is 10.23%,the 24 h atmospheric water absorption is 17.86%,and the porosity is 20.99%.The smaller the apparent density is,the larger the porosity is,and the water absorption performance is better.From the microscopic point of view,bonding ability between blue brick mineral particles is relatively weak.The pores between skeletons are large and the pore structure is obvious.From the perspective of material phase,the elements of blue brick are mainly O,Si,Al,Fe,and the composition of blue brick is mainly composed of quartz and feldspar.The softening coefficient of blue brick is 0.80,and the deformation and stability of the structure should be paid special attention in the rainy season or wet environment.Through the frost test,there are salt substances in the internal pores of the brick,and the surface of the blue brick is eroded and pulverized.In this paper,the experimental process and analysis methods for testing the material properties of blue brick can provide reference for the research on the material properties of the same kind of blue-brick masonry in historic buildings and masonry relics.The relevant material property parameters obtained in this paper can provide guidance for making protection schemes and scientific repairs for historic buildings in Central China,enrich the evaluation criteria for maintaining and reinforcing historic buildings,and provide theoretical support for studying the damage and health detection technology related to historic buildings.
基金Acknowledgments This research was jointly supported by National Science Foundation of China （No. 20777092）, the ministry of Science and Technology of China （2007CB407304） and Natural science foundation of Zhejiang province of China （Y307025）.
文摘The concept of“New Engineering”has put forward new challenges to the talents cultivation of universities.Due to some problems of the traditional Software Engineering curriculum,e.g.separated design at undergraduate-level and graduate-level courses,poor curriculum structure,lacking of industry characteristics.This paper proposes an integrated undergraduate-graduate education curriculum for Software Engineering Major,which is based on Software Engineering specialty knowledge system(C-SWEBOK)and focuses on the current national strategic demands.Additionally,the curriculum combines with the University’s transportation characteristics,and fuses the discipline of Software Engineering and Intelligent Transportation.The multi-level curriculum designed in this paper is with reasonable structure,complete system,progressive content,and salient feature,which provides the strong support for cultivating high-qualified software talents in line with national strategies and industry needs.
