【目的】研究低出生体重儿在生后24月龄内发生追赶性生长时其体质量指数(body mass index,BMI)的变化规律。【方法】采用出生队列方法,选择嘉定区2016年1月—2016年12月出生的户籍和常住儿童中自愿加入0~24月龄随访研究中的低出生体重儿...【目的】研究低出生体重儿在生后24月龄内发生追赶性生长时其体质量指数(body mass index,BMI)的变化规律。【方法】采用出生队列方法,选择嘉定区2016年1月—2016年12月出生的户籍和常住儿童中自愿加入0~24月龄随访研究中的低出生体重儿(出生体重<2500 g)126人,根据出生体重和孕周分为早产适于胎龄儿组73人、足月小于胎龄儿组53人;选择同地区、孕周37~41周、出生体重2500~3999 g 105人为对照组。比较3组婴幼儿生后0~24月龄间BMI均数、标准差的差异,分析男女婴幼儿在0~24月龄间BMI曲线的变化。【结果】①共纳入231人,男童111人,女童120人;②2组低出生体重儿在出生和2月龄阶段BMI均值均低于对照组,早产适于胎龄儿从4月龄开始BMI与对照组无明显差异,但是足月小于胎龄儿组BMI指数在4~18月龄间始终低于早产适于胎龄儿组别和对照组,差异有统计学意义(P<0.05);③3组婴幼儿BMI指数在生后均呈现快速上升,在4~6月龄时达高峰,7~9月龄BMI数值开始下降,早产适于胎龄儿在6月龄BMI的比较中便赶上正常体重儿童的BMI值,而足月小于胎龄儿一直到24月龄仍与正常体重儿童的BMI值有差异,但是3组间的差值有缩小。男女性别间BMI上升曲线相似,但早产适于胎龄儿女童组别高峰值延长。【结论】低出生体重儿的BMI存在追赶性增长现象,其后期变化趋势与正常出生体重儿相似。在儿童早期生长发育阶段,应给予低出生体重儿喂养方面的合理指导和干预。展开更多
Objective To observe the effect of electroacupuncture on the morphological change of the bladder tissue and the protein expression levels of cytochrome C, Caspase-3 and Caspase-9 in the bladder tissue of rats with neu...Objective To observe the effect of electroacupuncture on the morphological change of the bladder tissue and the protein expression levels of cytochrome C, Caspase-3 and Caspase-9 in the bladder tissue of rats with neurogenic bladder after suprasacral spinal cord injury, and to preliminarily explore its partial mechanism of action. Methods Forty-eight female SD rats were randomly divided into blank group（group B）, sham operation group（group S）, model group（group M） and electroacupuncture group（group EA） according to random number table method with 12 rats in each group. Neurogenic bladder models after suprasacral spinal cord injury were established by adopting modified spinal cord transection method. Electroacupuncture intervention was conducted at ＂Cìliáo（次髎 BL 32）＂＂Zhōngjí（中极 CV 3）＂ ＂Sānyīnjiāo（三阴交 SP 6）＂ and ＂Dàzhuī（大椎 GV 14）＂ on the 19 th day after modeling. After treatment for 7 consecutive days, the rats were sacrificed, and the bladder tissues were removed rapidly for microscopic observation of morphological change after HE stain and for determination of the protein expression levels of cytochrome C, Caspase-3 and Caspase-9 via Western blot method. Results① Under light microscope after HE stain, injury in varying degrees in muscle fiber arrangement and the shape and size of cells, etc. of bladder detrusor of rats in group M and group EA was found, and the degree of injury of detrusor in group M was severer than that in group EA;② comparison of the protein expression levels of cytochrome C, Pro-Caspase-9 and activated Caspase-9 between group M as well as group EA and group B as well as group S： the protein expression levels of cytochrome C, Pro-Caspase-9 and activated Caspase-9 in bladder tissue of rats in group M and group EA obviously increased（ P〈0.01, P〈0.05）; comparison between group EA and group M： the protein expression levels of cytochrome C, Pro-Caspase-9 and activated Caspase-9 in bladder tissue of rats in group EA obviously reduced（ P〈0.05）;③ comparison of the protein expression level of Caspase-3 between group M as well as group EA and group B as well as group S： the protein expression level of Pro-Caspase-3（35 KD） in group M and group EA reduced, and the protein expression level of activated Caspase-3（17 KD/19 KD） obviously increased（ P〈0.01, P〈0.05）; comparison between group EA andgroup M： the protein expression level of Pro-Caspase-3（35 KD） in group EA obviously increased, and the protein expression level of activated Caspase-3（17 KD/19 KD） obviously reduced（ P〈0.05）. Conclusion suprasacral spinal cord injury can increase the protein expression levels of cytochrome C, activated Caspase-3 and Caspase-9 in bladder tissues of rats, and electroacupuncture at BL 32, CV 3, SP 6 and GV 14 can down-regulate the protein expression levels of cytochrome C, activated Caspase-3 and Caspase-9 in bladder tissues of rats after suprasacral spinal cord injury and protect bladder tissue, which may be one of the mechanisms that electroacupuncture protects the involved bladder detrusor after suprasacral spinal cord injury.展开更多
文摘【目的】研究低出生体重儿在生后24月龄内发生追赶性生长时其体质量指数(body mass index,BMI)的变化规律。【方法】采用出生队列方法,选择嘉定区2016年1月—2016年12月出生的户籍和常住儿童中自愿加入0~24月龄随访研究中的低出生体重儿(出生体重<2500 g)126人,根据出生体重和孕周分为早产适于胎龄儿组73人、足月小于胎龄儿组53人;选择同地区、孕周37~41周、出生体重2500~3999 g 105人为对照组。比较3组婴幼儿生后0~24月龄间BMI均数、标准差的差异,分析男女婴幼儿在0~24月龄间BMI曲线的变化。【结果】①共纳入231人,男童111人,女童120人;②2组低出生体重儿在出生和2月龄阶段BMI均值均低于对照组,早产适于胎龄儿从4月龄开始BMI与对照组无明显差异,但是足月小于胎龄儿组BMI指数在4~18月龄间始终低于早产适于胎龄儿组别和对照组,差异有统计学意义(P<0.05);③3组婴幼儿BMI指数在生后均呈现快速上升,在4~6月龄时达高峰,7~9月龄BMI数值开始下降,早产适于胎龄儿在6月龄BMI的比较中便赶上正常体重儿童的BMI值,而足月小于胎龄儿一直到24月龄仍与正常体重儿童的BMI值有差异,但是3组间的差值有缩小。男女性别间BMI上升曲线相似,但早产适于胎龄儿女童组别高峰值延长。【结论】低出生体重儿的BMI存在追赶性增长现象,其后期变化趋势与正常出生体重儿相似。在儿童早期生长发育阶段,应给予低出生体重儿喂养方面的合理指导和干预。
基金Supported by National Natural Science Foundation of China:81473753
文摘Objective To observe the effect of electroacupuncture on the morphological change of the bladder tissue and the protein expression levels of cytochrome C, Caspase-3 and Caspase-9 in the bladder tissue of rats with neurogenic bladder after suprasacral spinal cord injury, and to preliminarily explore its partial mechanism of action. Methods Forty-eight female SD rats were randomly divided into blank group（group B）, sham operation group（group S）, model group（group M） and electroacupuncture group（group EA） according to random number table method with 12 rats in each group. Neurogenic bladder models after suprasacral spinal cord injury were established by adopting modified spinal cord transection method. Electroacupuncture intervention was conducted at ＂Cìliáo（次髎 BL 32）＂＂Zhōngjí（中极 CV 3）＂ ＂Sānyīnjiāo（三阴交 SP 6）＂ and ＂Dàzhuī（大椎 GV 14）＂ on the 19 th day after modeling. After treatment for 7 consecutive days, the rats were sacrificed, and the bladder tissues were removed rapidly for microscopic observation of morphological change after HE stain and for determination of the protein expression levels of cytochrome C, Caspase-3 and Caspase-9 via Western blot method. Results① Under light microscope after HE stain, injury in varying degrees in muscle fiber arrangement and the shape and size of cells, etc. of bladder detrusor of rats in group M and group EA was found, and the degree of injury of detrusor in group M was severer than that in group EA;② comparison of the protein expression levels of cytochrome C, Pro-Caspase-9 and activated Caspase-9 between group M as well as group EA and group B as well as group S： the protein expression levels of cytochrome C, Pro-Caspase-9 and activated Caspase-9 in bladder tissue of rats in group M and group EA obviously increased（ P〈0.01, P〈0.05）; comparison between group EA and group M： the protein expression levels of cytochrome C, Pro-Caspase-9 and activated Caspase-9 in bladder tissue of rats in group EA obviously reduced（ P〈0.05）;③ comparison of the protein expression level of Caspase-3 between group M as well as group EA and group B as well as group S： the protein expression level of Pro-Caspase-3（35 KD） in group M and group EA reduced, and the protein expression level of activated Caspase-3（17 KD/19 KD） obviously increased（ P〈0.01, P〈0.05）; comparison between group EA andgroup M： the protein expression level of Pro-Caspase-3（35 KD） in group EA obviously increased, and the protein expression level of activated Caspase-3（17 KD/19 KD） obviously reduced（ P〈0.05）. Conclusion suprasacral spinal cord injury can increase the protein expression levels of cytochrome C, activated Caspase-3 and Caspase-9 in bladder tissues of rats, and electroacupuncture at BL 32, CV 3, SP 6 and GV 14 can down-regulate the protein expression levels of cytochrome C, activated Caspase-3 and Caspase-9 in bladder tissues of rats after suprasacral spinal cord injury and protect bladder tissue, which may be one of the mechanisms that electroacupuncture protects the involved bladder detrusor after suprasacral spinal cord injury.
