采用滤纸培养皿法,以发芽率、发芽势、根长和苗长为指标,研究细叶旱芹(Cyclospermum leptophyllum(Pers.)Sprague ex Britton et P.Wilson)地上部化感物质对4种草坪草和5种牧草受体植物的种子萌发和幼苗生长的影响。结果表明,细叶旱芹...采用滤纸培养皿法,以发芽率、发芽势、根长和苗长为指标,研究细叶旱芹(Cyclospermum leptophyllum(Pers.)Sprague ex Britton et P.Wilson)地上部化感物质对4种草坪草和5种牧草受体植物的种子萌发和幼苗生长的影响。结果表明,细叶旱芹地上部化感物质对4种草坪草和5种牧草的种子萌发和幼苗生长均有抑制作用,其中对种子发芽势抑制最明显,抑制率最高达96.3%;其次是对幼苗根长的抑制作用,抑制率最高达91.1%。通过比较种子萌发和幼苗生长的测定指标,得出5种馏分对草坪草的化感作用为石油醚相>氯仿相>正丁醇相>乙酸乙酯相>水相,而对牧草的化感作用为石油醚相>乙酸乙酯相>氯仿相>正丁醇相>水相。说明不同受体植物对相同萃取相组分的化感作用强弱存在差异,细叶旱芹地上部化感物质主要存在于石油醚馏分中,可进一步从中分离提取活性物质。展开更多
A gsnl4 mutant characterized by small grain size,narrow leaf and low seed-setting rate was obtained by ethyl methane sulfonate(EMS)mutagenesis of a japonica rice variety Wuyunjing 21.Genetic analysis showed that gsnl4...A gsnl4 mutant characterized by small grain size,narrow leaf and low seed-setting rate was obtained by ethyl methane sulfonate(EMS)mutagenesis of a japonica rice variety Wuyunjing 21.Genetic analysis showed that gsnl4 is a loss-of-function mutant.A single-base mutation in gsnl4 resulted in the substitution of Ser to Asn in the Piwi domain of OsAGOlb protein.CRISPR/Cas9-mediated editing of OsAGOlb yielded a mutant phenotypically resembling gsnl4.Furthermore,miRNA-Seq analysis showed that the transcript expression levels of miRNAs in the signal transduction pathways related to pollen development,leaf morphology and honnone activation were significantly different between the gsnl4 mutant and the wild type(WT)plants.Several miRNAs were downregulated,and their target genes were upregulated in gsnl4 mutants.The auxin content in the root tips of the gsnl4 mutant decreased,and the expression of most auxin-related genes was altered.In summary,GSNL4 not only regulates organ development by controlling cell division and expansion,but also plays an important role in regulating auxin transport in rice.展开更多
文摘采用滤纸培养皿法,以发芽率、发芽势、根长和苗长为指标,研究细叶旱芹(Cyclospermum leptophyllum(Pers.)Sprague ex Britton et P.Wilson)地上部化感物质对4种草坪草和5种牧草受体植物的种子萌发和幼苗生长的影响。结果表明,细叶旱芹地上部化感物质对4种草坪草和5种牧草的种子萌发和幼苗生长均有抑制作用,其中对种子发芽势抑制最明显,抑制率最高达96.3%;其次是对幼苗根长的抑制作用,抑制率最高达91.1%。通过比较种子萌发和幼苗生长的测定指标,得出5种馏分对草坪草的化感作用为石油醚相>氯仿相>正丁醇相>乙酸乙酯相>水相,而对牧草的化感作用为石油醚相>乙酸乙酯相>氯仿相>正丁醇相>水相。说明不同受体植物对相同萃取相组分的化感作用强弱存在差异,细叶旱芹地上部化感物质主要存在于石油醚馏分中,可进一步从中分离提取活性物质。
基金supported by the National Natural Science Foundation of China(Grant Nos.31771887,31671761 and 32001491)supported by the National Natural Science Foundation of China(Grant Nos.31771887,31671761 and 32001491).
文摘A gsnl4 mutant characterized by small grain size,narrow leaf and low seed-setting rate was obtained by ethyl methane sulfonate(EMS)mutagenesis of a japonica rice variety Wuyunjing 21.Genetic analysis showed that gsnl4 is a loss-of-function mutant.A single-base mutation in gsnl4 resulted in the substitution of Ser to Asn in the Piwi domain of OsAGOlb protein.CRISPR/Cas9-mediated editing of OsAGOlb yielded a mutant phenotypically resembling gsnl4.Furthermore,miRNA-Seq analysis showed that the transcript expression levels of miRNAs in the signal transduction pathways related to pollen development,leaf morphology and honnone activation were significantly different between the gsnl4 mutant and the wild type(WT)plants.Several miRNAs were downregulated,and their target genes were upregulated in gsnl4 mutants.The auxin content in the root tips of the gsnl4 mutant decreased,and the expression of most auxin-related genes was altered.In summary,GSNL4 not only regulates organ development by controlling cell division and expansion,but also plays an important role in regulating auxin transport in rice.
