含引气剂海工混凝土的抗冻性能及其梁受弯承载力

为了研究引气剂对海工混凝土抗冻性能及其梁受弯承载力的影响,对2种配合比6根海工混凝土梁及相应的冻损试件进行总计100次快速冻融试验;采用质量损失率、动弹性模量损伤度和抗压强度损失率对混凝土冻融损伤程度进行有效评价;考虑混凝土抗压强度退化以及等效矩形应力图系数的变化,提出了冻融作用下海工混凝土梁正截面受弯承载力计算公式。结果表明:加入引气剂可以增强海工混凝土的抗冻性能,降低梁的开裂弯矩退化速率,但会降低混凝土强度和梁的极限弯矩;在150次冻融循环内,提出的模型能够较好地预测冻融作用下海工混凝土梁的正截面受弯承载力。

基于双端行波频率变化特性的中低速磁浮交通供电轨故障定位方法

中低速磁浮供电轨供电过程中容易发生直流接地故障,由于其供电距离较短、接地网结构复杂,发生直流接地故障后不易准确定位故障点。为此针对以上问题,根据列车运行过程中车体与轨道无接触的特点,建立中低速磁浮牵引供电系统,分析双端故障测距与单端故障测距的优缺点,研究供电轨发生接地故障后故障行波时域分布与频域分布的关系;并通过计算式计算分析行波频谱的产生机理,得出故障行波的频率分布不受车站内阻抗、过渡电阻的影响,只与故障点位置和故障行波到达线路两端的时间相关;基于此,提出基于双端频率变化特性的中低速磁浮交通供电轨故障定位方法,根据故障行波频率特性与行波速度计算故障点位置。并且通过仿真验证,该方法不受故障点位置、过渡电阻大小影响,且适用于单处接地故障、多处接地故障类型,故障误差始终保持在1%以内,相比传统的直流牵引供电系统故障定位方法,测量精度更高,适用于中低速磁浮交通供电轨接地故障。

Aberrant expression of ether à go-go potassium channel in colorectal cancer patients and cell lines

AIM: To study the expression of ether à go-go (Eag1) potassium channel in colorectal cancer and the relation- ship between their expression and clinico-pathological features. METHODS: The expression levels of Eag1 protein were determined in 76 cancer tissues with paired non- cancerous matched tissues as well as 9 colorectal adenoma tissues by immunohistochemistry. Eag1 mRNA expression was detected in 13 colorectal cancer tissues with paired non-cancerous matched tissues and 4 colorectal adenoma tissues as well as two colorectal cancer cell lines (LoVo and HT-29) by reverse transcription PCR. RESULTS: The frequency of positive expression of Eag1 protein was 76.3% (58/76) and Eag1 mRNA was 76.9% (10/13) in colorectal cancer tissue. Expression level of Eag1 protein was dependent on the tumor size, lymphatic node metastasis, other organ metastases and Dukes’ stage (P < 0.05), while not dependent on age, sex, site and degree of differentiation. Eag1 protein and mRNA were negative in normal colorectal tissue, and absolutely negative in colorectal adenomas except that one case was positively stained for Eag1 protein. CONCLUSION: Eag1 protein and mRNA are aberrantly expressed in colorectal cancer and occasionally expressed in colorectal adenoma. The high frequency of expression of Eag1 in tumors and the restriction of normal expression to the brain suggest the potential of this protein for diagnostic, prognostic and therapeutic purposes.

高温作用后GFRP/BFRP筋拉伸性能试验研究及强度折减计算

为探讨高温作用后2类纤维增强复合材料(FRP筋材)的拉伸性能退化情况,本文对高温作用后的玻璃/玄武岩纤维增强复合材料(GFRP/BFRP)筋材进行了拉伸试验,并将宏观与微观结合进行分析。试验结果表明:高温超过220℃后,筋材表面部分纤维开始脱落,整体性逐渐遭到破坏,已不再适合进行拉伸试验;经220℃以内温度作用后,2类FRP筋材抗拉强度均随作用温度升高呈阶段性下降的趋势;而弹性模量总体变化幅度很小;高温作用后,直径16 mm的GFRP筋抗拉强度下降速率比12 mm的2种FRP筋更快;结合试验结果与扫描电镜微观结构分析,可认为高温作用后2类FRP筋抗拉强度退化的主要原因是树脂和纤维界面黏结性能的退化。结合本文和已有相关试验结果,提出了20~220℃温度范围内G/BFRP筋抗拉强度的折减计算模型,并给出了关键温度节点对应的强度保留率ηt建议值。

Ca2+/calmodulin-dependent protein kinase II regulates colon cancer proliferation and migration via ERK1/2 and p38 pathways

AIM To investigate the role of calmodulin-dependent protein kinase Ⅱ(Ca MKⅡ) in colon cancer growth,migration and invasion.METHODS Ca MKⅡ expression in colon cancer and paracancerous tissues was evaluated via immunochemistry. Transcriptional and posttranscriptional levels of Ca MKⅡin tissue samples and MMP2,MMP9 and TIMP-1 expression in the human colon cancer cell line HCT116 were assessed by q RTPCR and western blot. Cell proliferation was detected with the MTT assay. Cancer cell migration and invasion were investigated with the Transwell culture system and woundhealing assay.RESULTS We first demonstrated that CaMK Ⅱ was ove rexpressed in human colon cancers and was associated with cancer differentiation. In the human colon cancer cell line HCT116,the Ca MKII-specific inhibitor KN93,but not its inactive analogue KN92,decreased cancer cell proliferation. Furthermore,KN93 also significantly prohibited HCT116 cell migration and invasion. The specific inhibition of ERK1/2 or p38 decreased the proliferation and migration of colon cancer cells.CONCLUSION Our findings highlight Ca MKⅡ as a potential critical mediator in human colon tumor development and metastasis.

OSL chronology and paleoclimatic implications of paleodunes in the middle and southwestern Qaidam Basin, Qinghai-Tibetan Plateau

Aeolian sediments are widely distributed in the Qaidam Basin, northeastern Qinghai-Ti^tan Plateau （QTP）. The pale（xiunes are mainly located at the southeastern, middle and southwestem Qaidam Basin. However, ages ofpaleodunes in the middle and southwestern Qaidam Basin have not been well studied, although they are close to and might connect to the evolution of salt lakes in the cenWal basin. In this study, we use single aliquot regeneration （SAR） protocol of Optically Stimulated Luminescence （OSL） to date the dune sand in these two regions. The results show that： （1） Sand accumulation in these regions started at ca. 4-3 ka and lasted to ca. 0.5 ka when they were stabilized, due to the arid climate in the late Holocene. （2） The underlying fluvial sand was fomaed during deghcialion at 12.6±0.8 ka. （3） The stabilization pe- riods of the paleodunes correspond to stages of glacier advance in the northeastem QTP, during which lower temperatures caused the decrease of evapomlion and increase of the effective moisttwe, leading to an increase of vegetation cover and stabilizalion of the dunes.

Effects of high-pressure rheo-squeeze casting on the Fe-rich phases and mechanical properties of Al-17Si-(1,1.5)Fe alloys

The effects of high pressure rheo-squeeze casting(HPRC) on the Fe-rich phases(FRPs) and mechanical properties of Al-17 Si-(1,1.5)Fe alloys were investigated. The alloy melts were first treated by ultrasonic vibration(UV) and then formed by high-pressure squeeze casting(HPSC). The FRPs in the as-cast HPSC Al-17 Si-1 Fe alloys only contained a long, needle-shaped β-Al5 Fe Si phase at 0 MPa. In addition to the β-Al5 Fe Si phase, the HPSC Al-17 Si-1.5 Fe alloy also contained the plate-shaped δ-Al4 Fe Si2 phase. A fine, block-shaped δ-Al4 Fe Si2 phase was formed in the Al-17 Si-1 Fe alloy treated by UV. The size of FRPs decreased with increasing pressure. After UV treatment, solidification under pressure led to further refinement of the FRPs. Considering alloy samples of the same composition, the ultimate tensile strength(UTS) of the HPRC samples was higher than that of the HPSC samples, and the UTS increased with increasing pressure. The UTS of the Al-17 Si-1 Fe alloy formed by HPSC exceeded that of the Al-17 Si-1.5 Fe alloy formed in the same manner under the same pressure. Conversely, the UTS of the Al-17 Si-1 Fe alloy formed by HPRC decreased to a value lower than that of the Al-17 Si-1.5 Fe alloy formed in the same manner.

In situ staining of the primo vascular system in the ventricles and subarachnoid space of the brain by trypan blue injection into the lateral ventricle

We examined a new method for visualization of the primo vascular system in the rat brain involving lateral ventricle injection of trypan blue. Results showed that the primo vascular system in the lateral ventricles and arachnoid mater of the brain were preferentially stained relative to blood vessels and fascia. The primo-vessels along blood vessels in the brain were clearly exhibited. In addition, the primo vascular system was evident between the fourth ventricle and the quadrigeminal cistern. Our experimental findings indicate that this new technique of lateral ventricle injection of trypan blue can visualize the primo vascular system in lateral ventricles and arachnoid mater of rats in situ.

KN-93,a specific inhibitor of CaMKⅡ inhibits human hepatic stellate cell proliferation in vitro

AIM: To investigate the effects of KN-93, a CaMKⅡ selective inhibitor on cell proliferation and the expression of p53 or p21 protein in human hepatic stellate cells. METHODS: Human hepatic stellate cells (LX-2) were incubated with various concentrations (0-50 mmol/L) of KN-93 or its inactive derivative, KN-92. Cell proliferation was measured by CCK-8 assay, and the expression of two cell cycle regulators, p53 and p21, was determined by SDS-PAGE and Western blotting. RESULTS: KN-93 (5-50 mmol/L) decreased the proliferation of human hepatic stellate cells in a dose- dependent manner from 81.76% (81.76% ± 2.58% vs 96.63% ± 2.69%, P < 0.05) to 27.15% (27.15% ± 2.86% vs 96.59% ± 2.44%, P < 0.01) after 24 h treatment. Incubation of 10 mmol/L KN-93 induced the cell growth reduction in a time-dependent manner from 78.27% at 8 h to 11.48% at 48 h. However, KN-92, an inactive derivative of KN-93, did not inhibit cell proliferation effectively. Moreover, analysis of cell cycle regulator expression revealed that KN-93 rather than KN-92 reduced the expression of p53 and p21.

Nano-SiC_P particles distribution and mechanical properties of Al-matrix composites prepared by stir casting and ultrasonic treatment

Nano-ceramic particles are generally difficult to add into molten metal because of poor wettability. Nano-SiC_Particles reinforced A356 aluminum alloy composites were prepared by a new complex process, i.e., a molten-metal process combined with high energy ball milling and ultrasonic vibration methods. The nano particles were β-SiC_P with an average diameter of 40 nm, and pre-oxidized at about 850 °C to form an oxide layer with a thickness of approximately 3 nm. The mm-sized composite granules containing nano-SiC_P were fi rstly produced by milling the mixture of oxidized nano-SiC_P and pure Al powders, and then were remelted in the matrix-metal melt with mechanical stirring and treated by ultrasonic vibration to prepare the composite. SEM analysis results show that the nano-SiC_P articles are distributed uniformly in the matrix and no serious agglomeration is observed. The tensile strength and elongation of the composite with 2 wt.% nano-SiC_P in as-cast state are 226 MPa and 5.5%, improved by 20% and 44%, respectively, compared with the A356 alloy.

Diffuse large B cell lymphoma with bilateral adrenal and hypothalamic involvement: A case report and literature review

BACKGROUND Non-Hodgkin’s lymphoma(NHL)can involve extralymphatic organs,resulting in diverse clinical manifestations,especially if the endocrine organs are affected.This type of involvement can often be difficult to detect accurately.Until now,no patients with NHL and concomitant bilateral adrenal and hypothalamic involvement have been reported.The purpose of this article is to discuss the diagnosis and treatment of lymphoma with bilateral adrenal gland and hypothalamic involvement so as to help physicians avoid misdiagnosis and missed diagnosis.CASE SUMMARY We describe a case of a 52-years-old male patient with bilateral adrenal masses,who presented with a fever of unknown origin on admission.Subsequently,hypopituitarism of the anterior pituitary followed by posterior pituitary developed.18fluorine-fluorodeoxyglucose positron emission tomography/computed tomography(PET/CT)showed lesions with a high metabolism in both adrenal glands,hypothalamus,left supraclavicular lymph nodes,and other organs.The etiological diagnosis was determined based on a left supraclavicular lymph node biopsy.The patient,who eventually present with panhypopituitarism,was finally diagnosed with diffuse large B cell lymphoma with bilateral adrenal gland and hypothalamic involvement.After immunochemotherapy,glucocorticoids administration and desmopressin acetate replacement therapy,the symptoms of fever and panhypopituitarism improved,and all the lesions reduced in size.CONCLUSION This report demonstrates that,although synchronous involvement of two endocrine organs is rare in NHL,extra caution should be taken when dysfunction occurs in multiple endocrine organs.

Persistence of four dominant psammophyte species in central Inner Mongolia of China under continual drought

Clarifying the persistence time of seedlings of dominant species under continual drought will help us understand responses of ecosystems to global climate change and improve revegetation efforts. Drought tolerance of four dominant psammophytic shrub species occurring in different environments was studied in the semi-arid areas of Inner Mongolian grasslands. Seedlings of Hedysarum laeve, Caragana korshinskii, Artemisia sphaerocephala and Artemisia ordosica were grown under four air temperature regimes （night/day： 12.5/22.5℃, 15/25℃, 17.5/27.5℃ and 20/30℃） within climate （air temperature and humidity） controlled, naturally lit glasshouses with a night/day relative humidity of 70%/50%. Pots were watered to field capacity for each temperature treatment. Soil water condition was monitored by weighting each pot every day using an electronic balance. Date of seedling death for each treatment was recorded and the dead plants were harvested. Plant dry weights were determined after oven drying at 80℃ for 3 days. Two Artemisia species had higher growth rates than H. laeve and C. korshinskii, and the growth of all four species increased with increasing temperatures. The two Artemisia species had the highest leaf biomass increment, followed by C. korshinskii, and then H. laeve. Shoot biomass increment was higher for A. ordosica and C. korshinskii, intermediate for A. sphaerocephala and lowest for H. laeve. C. korshinskii had the highest root biomass increment. The final soil water content at death for all four species varied from 1% to 2%. C. korshinskii, A. sphaerocephala, H. laeve and A. ordosica survived for 25-43, 24-41, 26-41 and 24-37 days without watering, respectively. C. korshinskii, A. sphaerocephala, H. Laeve, and A. ordosica seedlings survived longer at the lowest temperatures （12.5/22.5℃） than at the highest temperatures （20/30℃） by 18, 17, 15 and 13 days, respectively. Increased climatic temperatures induce the death of seedlings in years with long intervals between rainfall events. The adaptation of seedlings to droughts should be emphasized in revegetation efforts in the Ordos Plateau, Inner Mongolia.

对基于动弹性模量的海工混凝土抗冻损伤评价

为分析冻融环境下海工混凝土性能的退化规律,对三种配合比的海工混凝土进行了快速冻融试验,并对其物理力学性能及氯离子渗透性能进行分析与评估.试验结果表明:当冻融循环分别超过25次和50次后,海工混凝土的内部损伤和表面损伤先后出现明显的加剧;总掺量不变但矿粉含量较多时,海工混凝土的抗冻性较优,且掺入适量纳米SiO_(2)能进一步有效提高混凝土的抗冻性;以动弹性模量损伤度为依据,对冻融循环作用下海工混凝土的抗压强度损失率及氯离子迁移系数增长率进行分析;并对动弹性模量损伤度与冻融循环次数的直接关系进行了探讨.分析结果表明经历冻融作用的海工混凝土,其动弹性模量损伤度与抗压强度损失率、氯离子迁移系数增长率之间存在较好的线性关系,且幂函数能有效地反映动弹性模量损伤度与冻融循环次数之间的关系.

LOADING SEQUENCE AND ARRANGEMENT EFFECTS FOR TWO－DIMENSIONAL DISTRIBUTIONAL SPECTRUM

AbstractThe loading sequence effect in constructing a fatigue loading spectrum has been paidattention to by experts at home and abroad for many years. In this paper, loading sequcnceand arrangement effects are analysed for two dimensional distributional spectrum. It isderived that loading sequence effect is little concerned with the approaches to constructingfatigue spectrum and varies only along with the total mean value of a loading spectrumthough it has a great effect on fatigue lives of components. So the loading arrangement ef-fect is small, and the approaches to constructing fatigue spectrum have little effect on lives.

TREATMENT OF COMPLEX LOAD HISTORY AND CONSTRUCTION OF FATIGUE LOAD SPECTRUM

A new, more actual approach for omitting small loads in a loading history was pres-ented according to crack closure, and load interactive effects which are large on damage of com-ponents were studied. Intrinsic periodic spectrum block representing a damage element of a loadhistory, a new concept, was put out. It has been proven by theoretic analysis and tests that themodes of constructing fatigue loading spectrum have little effect on damage of components,which will change the incomplete knowledges on constructing fatigue spectrum ago.

直流应力下主动式微粒抑制方法的动态配合研究

直流气体绝缘输电线路(GIL)中自由金属微粒的活性较之交流GIL中更为活跃,对直流GIL的技术发展以及应用带来了严峻的挑战。目前,将各种抑制微粒手段进行主动式动态配合设计是未来发展的趋势。该文搭建直流GIL金属微粒主动式抑制的动态配合实验平台,并结合有限元仿真,从陷阱捕获率的角度优化了驱赶电极与陷阱间的位置配合;同时提出直流老练优化程序,最终获得主动式微粒抑制的动态配合有效方案。研究结果表明,当驱赶电极与陷阱间的距离为18 mm时,陷阱捕获率可高达70%;该文优化后的直流老练程序较传统程序可使陷阱捕获性能提升50%以上;最后通过实验验证了驱赶电极的有效性,且通过大量实验发现,高压电极布置驱赶电极后,陷阱捕获率可提高50%以上。因此,该文结果对提升直流GIL抑制金属微粒的能力具有一定的参考价值。

贸易高质量发展评价指标体系构建研究

本文基于中共中央、国务院发布的《关于推进贸易高质量发展的指导意见》,结合新发展理念和我国贸易高质量发展目标,从贸易开放水平、结构协调水平、贸易可持续水平、国际竞争力水平和创新驱动水平五个维度综合构建我国贸易高质量发展评价指标体系。通过阐述相关指标的定义,选取15个二级指标进行度量,并结合可实施的数据处理方法和测算方法,提出了针对该指标体系的相关运用建议,以期进一步明确新时代我国贸易高质量发展的内涵特征,充分释放贸易高质量发展的潜力,丰富和补充我国贸易高质量发展理论体系和相关成果。

直流电压下绝缘子附近纤维的吸附运动行为及其对表面电荷积聚的影响

从近几年交流气体绝缘隔离开关(gas insulated switchgear,GIS)现场运行情况发现,纤维也可引起设备绝缘故障,而直流单极性场下,纤维的带电更为充分,运动也将更为活跃,且和绝缘子表面电荷有着强烈的交互作用。为此通过搭建半封闭式同轴圆柱电极实验平台,研究了直流电压下纤维在电极上充电效应,以及在盆式绝缘子附近吸附运动特性,最后使用粉尘图法,分析了纤维附着状态下对绝缘子表面电荷积聚的影响程度。研究结果表明,纤维由充电效应在启举过程中表现时滞特性,并实验验证了纤维充电特性。纤维起跳后在气隙中呈往复摆动向上的吸附运动特性,当纤维长度较长或离绝缘子较远时,起跳后的径向速度较小,且由于受到地电极静电吸附的影响运动高度很低,从而被吸附于绝缘子中下部表面;相反,纤维起跳后径向速度大,首次与绝缘子表面碰撞时攀升或被反弹,最终吸附于绝缘子中上部表面。通过粉尘图得出纤维附着状态下长期耐压后,将呈现“伞状”电荷分布,且与高压电极接触时,电荷分布范围广且积聚量最多;与地电极接触时次之;附着于绝缘子中间部位时最小。

局部特征聚类联合区域增长的桥梁裂缝检测

针对传统裂缝检测算法抗干扰能力弱,浅层裂缝易误判等问题,提出一种局部特征聚类联合区域增长的桥梁裂缝检测算法。首先,针对混凝土表皮脱落及渗水等干扰问题,采用Gauss-Frangi双重滤波对图像模糊化处理,退化噪声的特征信息,并增强图像中的线性结构。其次,针对常规算法无法识别弱特征的浅层裂缝问题,根据局部区域裂缝点间的空间相关性,提出基于网格聚类联合区域增长算法实现局部区域裂缝的动态分割。最后,针对分割图像中伪裂缝等顽固噪声,提出一种基于形状特征及结构相似性原理方法剔除噪声。实验表明,所提算法可检测出更多的裂缝细节信息,且保持较高的精确率,提高了裂缝图像分割质量。

带有变采样间隔特性的改进型EWMA■控制图设计

文章在改进型指数加权移动平均(IEWMA)■控制图的基础上,考虑样本均值在控制图受控区域的实际位置,提出了一种变采样间隔(VSI)IEWMA■控制图。采用马尔科夫链构建了VSI IEWMA■控制图的状态转移矩阵,并推导其平均报警样本个数和平均报警时间。仿真结果表明,针对不同的均值偏移大小,引入VSI特性显著提高了传统固定采样间隔IEWMA■控制图的性能,且通过与传统VSI EWMA■控制图比较可以发现,VSI IEWMA■控制图的性能更加优越,尤其是针对过程均值参数产生较小偏移的情形。