The title compound 6?-chloro-4?-methylene-3 H-spiro [isoben zofura-1,3?-isochroman], C_(17)H_(13)ClO_2, was prepared and characterized by IR, NMR, HRMS and single-crystal X-ray diffraction. This compound has a bisbenz...The title compound 6?-chloro-4?-methylene-3 H-spiro [isoben zofura-1,3?-isochroman], C_(17)H_(13)ClO_2, was prepared and characterized by IR, NMR, HRMS and single-crystal X-ray diffraction. This compound has a bisbenzannulated [5,5]-spiroketals skeleton. It crystallizes in orthorhombic, space group P212121 with a = 13.383(3), b = 7.6120(17), c = 13.793(3) ?, Z = 4, Dc= 1.275 g/cm^3, μ = 0.28 mm^(–1), F(000) = 592, wR = 0.1443 and R = 0.0557. The preliminary biological test showed that the title compound has good anti-bacterial activities in vitro.展开更多
To study the influence of Hypericum perforatum extract （HPE） on piglets infected with porcine respiratory and reproductive syndrome virus （PRRSV）, enzyme-labeled immunosorbent assay （ELISA） and cytopathic effect...To study the influence of Hypericum perforatum extract （HPE） on piglets infected with porcine respiratory and reproductive syndrome virus （PRRSV）, enzyme-labeled immunosorbent assay （ELISA） and cytopathic effect （CPE） were used to determine in vitro whether HPE could induce swine pulmonary alveolar macrophages （PAMs） to secrete IFN-γ, and whether PRRSV titers in PAMs were affected by the levels of HPE-induced IFN-γ. HPE （200 mg·kg-1） was administrated by oral gavage to piglets infected with the PRRSV in vivo to observe whether HPE affected the viremia, lung viral titers, and weight gain of piglets infected with PRRSV. The results showed that HPE was capable of inducing PAMs to produce IFN-γ in a dose dependent manner and HPE pretreatment was capable of significantly reducing PRRSV viral titers in PAMs （P〈 0.01）. Administration of HPE to the PRRSV-infected animals significantly （P〈 0.05） reduced viremia over time as compared with the PRRSV-infected animals. But there was not significant decrease in lung viral titers at day 21 post-infection between the HPE- treated animals and the PRRSV-infected control piglets. There were no significant differences in weight gain over time among the HPE-treatment animals, the normal control, and the HPE control animals. The PRRSV-infected animals caused significant （P〈 0.01） growth retardation as compared with the HPE controls and the normal piglets. It suggested that HPE might be an effective novel therapeutic approach to diminish the PRRSV-induced disease in swine.展开更多
基金National Key Technology Support Program(No.2015BAD11B02)Agricultural Science and Technology Innovation Program(ASTIP,No.CAASASTIP-2014-LIHPS-04)
文摘The title compound 6?-chloro-4?-methylene-3 H-spiro [isoben zofura-1,3?-isochroman], C_(17)H_(13)ClO_2, was prepared and characterized by IR, NMR, HRMS and single-crystal X-ray diffraction. This compound has a bisbenzannulated [5,5]-spiroketals skeleton. It crystallizes in orthorhombic, space group P212121 with a = 13.383(3), b = 7.6120(17), c = 13.793(3) ?, Z = 4, Dc= 1.275 g/cm^3, μ = 0.28 mm^(–1), F(000) = 592, wR = 0.1443 and R = 0.0557. The preliminary biological test showed that the title compound has good anti-bacterial activities in vitro.
基金supported by One Hundred Person Project of the Chinese Academy of Sciences (Renjiaozi[2008] 287)the Special Fund to Aid Basic Scientific Research of State Level Research Institutes for Public Welfare, China (BRF070402)
文摘To study the influence of Hypericum perforatum extract （HPE） on piglets infected with porcine respiratory and reproductive syndrome virus （PRRSV）, enzyme-labeled immunosorbent assay （ELISA） and cytopathic effect （CPE） were used to determine in vitro whether HPE could induce swine pulmonary alveolar macrophages （PAMs） to secrete IFN-γ, and whether PRRSV titers in PAMs were affected by the levels of HPE-induced IFN-γ. HPE （200 mg·kg-1） was administrated by oral gavage to piglets infected with the PRRSV in vivo to observe whether HPE affected the viremia, lung viral titers, and weight gain of piglets infected with PRRSV. The results showed that HPE was capable of inducing PAMs to produce IFN-γ in a dose dependent manner and HPE pretreatment was capable of significantly reducing PRRSV viral titers in PAMs （P〈 0.01）. Administration of HPE to the PRRSV-infected animals significantly （P〈 0.05） reduced viremia over time as compared with the PRRSV-infected animals. But there was not significant decrease in lung viral titers at day 21 post-infection between the HPE- treated animals and the PRRSV-infected control piglets. There were no significant differences in weight gain over time among the HPE-treatment animals, the normal control, and the HPE control animals. The PRRSV-infected animals caused significant （P〈 0.01） growth retardation as compared with the HPE controls and the normal piglets. It suggested that HPE might be an effective novel therapeutic approach to diminish the PRRSV-induced disease in swine.
