Background:The high dependence of intensive ruminant production on soybean meal and the environmental impact of this crop encourage the search for alternative protein-rich feeds.The use of insects seems promising,but ...Background:The high dependence of intensive ruminant production on soybean meal and the environmental impact of this crop encourage the search for alternative protein-rich feeds.The use of insects seems promising,but the extent of their ruminal protein degradation is largely unknown.This parameter has major influence not only on N utilization efficiency but also on the environmental burden of ruminant farming.In addition,although assessing ruminal N degradation represents a key first step to examine the potential of new feeds,it is a challenging task due to the lack of a reference method.This study was conducted to investigate the potential of 4 insects(Tenebrio molitor,Zophobas morio,Alphitobius diaperinus and Acheta domesticus)as alternative protein sources for ruminants,using 3 methodologies:1)a regression technique based on the in vitro relationship between gas production and ammonia-N concentration;2)a conventional in vitro technique of batch cultures of ruminal microorganisms,based on filtering the incubation residue through sintered glass crucibles;and 3)the in situ nylon bag technique.The in vitro intestinal digestibility of the non-degraded protein in the rumen was also determined.Soybean meal was used as a reference feedstuff.Results:Comparison of evaluation methods(regression,in vitro and in situ)did not allow to reliably select a single value of ruminal N degradation for the studied substrates,but all techniques seem to establish a similar ranking,with good correlations between methods,particularly between regression and in situ results.Regardless of the methodology,nitrogen from the 4 insects(with contents ranging from 81 to 112 g/kg of dry matter)did not show high ruminal degradation(41–76%),this value being always lower than that of soybean meal.Furthermore,the in vitro intestinal digestibility of non-degraded N was relatively high in all feeds(≥64%).Conclusion:Overall,these results support the potential of the 4 studied insects as alternative feedstuffs for ruminants.Among them,T.molitor showed the lowest and greatest values of ruminal N degradation and intestinal digestibility,respectively,which would place it as probably the best option to replace dietary soybean meal and increase the sustainability of ruminant feeding.展开更多
基金conducted within the framework of project PIE 202040E100 (CSIC, Spanish National Research Council)Pablo G. Toral benefited from a Ramón y Cajal research contract (RYC-2015-17230), from the Spanish Ministry of Economy and Competitiveness (MINECO), co-funded by the European Social FundLizbeth E. Robles-Jiménez was granted a SEGIB-Fundación Carolina fellowship
文摘Background:The high dependence of intensive ruminant production on soybean meal and the environmental impact of this crop encourage the search for alternative protein-rich feeds.The use of insects seems promising,but the extent of their ruminal protein degradation is largely unknown.This parameter has major influence not only on N utilization efficiency but also on the environmental burden of ruminant farming.In addition,although assessing ruminal N degradation represents a key first step to examine the potential of new feeds,it is a challenging task due to the lack of a reference method.This study was conducted to investigate the potential of 4 insects(Tenebrio molitor,Zophobas morio,Alphitobius diaperinus and Acheta domesticus)as alternative protein sources for ruminants,using 3 methodologies:1)a regression technique based on the in vitro relationship between gas production and ammonia-N concentration;2)a conventional in vitro technique of batch cultures of ruminal microorganisms,based on filtering the incubation residue through sintered glass crucibles;and 3)the in situ nylon bag technique.The in vitro intestinal digestibility of the non-degraded protein in the rumen was also determined.Soybean meal was used as a reference feedstuff.Results:Comparison of evaluation methods(regression,in vitro and in situ)did not allow to reliably select a single value of ruminal N degradation for the studied substrates,but all techniques seem to establish a similar ranking,with good correlations between methods,particularly between regression and in situ results.Regardless of the methodology,nitrogen from the 4 insects(with contents ranging from 81 to 112 g/kg of dry matter)did not show high ruminal degradation(41–76%),this value being always lower than that of soybean meal.Furthermore,the in vitro intestinal digestibility of non-degraded N was relatively high in all feeds(≥64%).Conclusion:Overall,these results support the potential of the 4 studied insects as alternative feedstuffs for ruminants.Among them,T.molitor showed the lowest and greatest values of ruminal N degradation and intestinal digestibility,respectively,which would place it as probably the best option to replace dietary soybean meal and increase the sustainability of ruminant feeding.
