Microbiological Assessment of Soil Planted with Cover Crops, and Soybean and Maize in Succession

This study aimed to assess the population density of nematodes and mycorrhizal soil fungi, in areas cultivated with oats, brachiaria, forage and white lupine, as well as in maize and soybean crops in succession, in order to generate a microbiological indicator of soil quality. In order to assess nematode and arbuscular mycorrhizal fungi (AMF) population densities, the experiment was performed in two stages: the first assessment was performed in the area where different cover crops were planted, in five seasons (0, 60, 90, 120, 150 days after sowing—DAS). In the second stage, soybean and maize crops in succession were assessed. According to the results, free-living nematodes and arbuscular mycorrhizal fungi population densities were not affected by the cover crop species used and, therefore, these can be grown prior to soybean and corn crops, without impact to free-living nematodes and AMF. The largest population of saprophyte nematodes and AMF occurred at 90 days. The cultivation of soybean and corn did not influence the number of free-living nematodes, but influenced the number of arbuscular mycorrhizal fungi. The highest numbers of mycorrhizal fungi Gigaspora margarita and Glomus macrocarpum were found in maize.

Production of Lettuce Seedlings with Association of Diazotrophic Bacteria and Humic Acids

The objective of this work was to verify the responses in agronomic and physiological characters of lettuce seedlings when submitted to different doses of humic acids and the presence or absence of inoculation of the bacterium Azospirillum brasilense. The experiment was carried out at the Protected Cultivation Station and Biological Control Teacher Mário César Lopes, belonging to the Experimental Stations Nucleus of the State University of the West of Paraná. The experimental design was a randomized block design, in a 5 × 2 factorial scheme, with four repetitions, totaling 40 plots. The first factor consisted of five solutions containing humic acid (100%, 75%, 50%, 25% and 0%) and the second factor was the presence or absence of the diazotrophic bacterium Azospirillum brasilense obtained by commercial product. Germination tests were carried out in addition to the determination of agronomic characters such as the number of fully expanded leaves, plant height, stem diameter, main root length, shoot dry mass, root dry mass, total dry mass, and Dickson quality index. The association of diazotrophic bacteria and humic acids did not influence the emergency rate, mean emergence time, mean speed of emergency, emergence speed index, plant height, and root length of lettuce seedlings. The dilutions of humic acids, regardless of inoculation with bacteria diazotrophic favored the development of the number of leaves, stem diameter and Dickson quality index. Inoculation of lettuce seeds with diazotrophic bacteria associated with the addition of humic acids, favored the development of the dry mass of root and total dry matter.