Objective:To study the bio-potency of epidermal mucus fromEpinephelus tauvina.Methods:Mucus was extracted with acidic,organic and aqueous solvents.Protein,carbohydrate,lipid,amino acid and fatty acid content of mucus ...Objective:To study the bio-potency of epidermal mucus fromEpinephelus tauvina.Methods:Mucus was extracted with acidic,organic and aqueous solvents.Protein,carbohydrate,lipid,amino acid and fatty acid content of mucus extracts were quantified by UV-spectrophotometer,high performance liquid chromatography and gas chromatography-mass spectrometer,respectively.Antimicrobial activity was tested against five human and fish pathogens by using agar well diffusion method.The molecular weight of peptides was determined using sodium dodecyl sulfate-polyacrylamide gel electrophoresis.The haemolytic activity of extracts was tested against chick,goat,cow and human red blood cell.Results:Protein contributed with maximum of 26.25%in crude mucus.Arginine was recorded maximum of(133.9 nmol/mL)in crude mucus.2,4,6-Decatrienoic acid and bis(a-chloroethyl)sulfone were confirmed in organic extract.The antimicrobial activity of acidic extract was significant.Among the human pathogens,maximum zone of inhibition[(26.0±0.3)mm]was observed againstProteus mirabilis.Whereas,among fish pathogens maximum zone of inhibition[(25.0±0.1)mm]was observed againstVibrio parahemolyticus.The activity of other two extracts was not remarkable.The molecular weight of peptides ranged from 115.5-37.1 kDa in acidic extract was determined.Chicken and goat blood were found to be highly vulnerable to the lysis.Conclusions:The whole mucus could be a promising source with numerous bioactive-potency.Consequently,this preliminary information suggested that mucus is a source of novel antimicrobial agents for fish and human health related applications.展开更多
基金Supported by the MoES(CMLRE)Ministry of Earth Sciences(Centre for Marine Living Resources and Ecology)(Grand No.10-IT IS/17/2012 dt.01/10/2012).
文摘Objective:To study the bio-potency of epidermal mucus fromEpinephelus tauvina.Methods:Mucus was extracted with acidic,organic and aqueous solvents.Protein,carbohydrate,lipid,amino acid and fatty acid content of mucus extracts were quantified by UV-spectrophotometer,high performance liquid chromatography and gas chromatography-mass spectrometer,respectively.Antimicrobial activity was tested against five human and fish pathogens by using agar well diffusion method.The molecular weight of peptides was determined using sodium dodecyl sulfate-polyacrylamide gel electrophoresis.The haemolytic activity of extracts was tested against chick,goat,cow and human red blood cell.Results:Protein contributed with maximum of 26.25%in crude mucus.Arginine was recorded maximum of(133.9 nmol/mL)in crude mucus.2,4,6-Decatrienoic acid and bis(a-chloroethyl)sulfone were confirmed in organic extract.The antimicrobial activity of acidic extract was significant.Among the human pathogens,maximum zone of inhibition[(26.0±0.3)mm]was observed againstProteus mirabilis.Whereas,among fish pathogens maximum zone of inhibition[(25.0±0.1)mm]was observed againstVibrio parahemolyticus.The activity of other two extracts was not remarkable.The molecular weight of peptides ranged from 115.5-37.1 kDa in acidic extract was determined.Chicken and goat blood were found to be highly vulnerable to the lysis.Conclusions:The whole mucus could be a promising source with numerous bioactive-potency.Consequently,this preliminary information suggested that mucus is a source of novel antimicrobial agents for fish and human health related applications.
