In recent years, proteomics has played a key role in identifying changes in protein levels in plant hosts upon infection by pathogenic organisms and in characterizing cellular and extracellular virulence and pathogeni...In recent years, proteomics has played a key role in identifying changes in protein levels in plant hosts upon infection by pathogenic organisms and in characterizing cellular and extracellular virulence and pathogenicity factors produced by pathogens. Proteomics offers a constantly evolving set of novel techniques to study all aspects of protein structure and function. Proteomics aims to find out the identity and amount of each and every protein present in a cell and actual function mediating specific cellular processes. Structural proteomics elucidates the development and application of experimental approaches to define the primary, secondary and tertiary structures of proteins, while functional proteomics refers to the development and application of global (proteome wide or system-wide) experimental approaches to assess protein function. A detail understanding of plant defense response using successful combination of proteomic techniques and other high throughput techniques of cell biology, biochemistry as well as genomics is needed for practical application to secure and stabilize yield of many crop plants. This review starts with a brief introduction to gel- and non gel-based proteomic techniques followed by the basics of plant-pathogen interaction, the use of proteomics in recent pasts to decipher the mysteries of plant-pathogen interaction, and ends with the future prospects of this technology.展开更多
Population explosion in the last decades together with global industrialization has caused heavy-metal contamination of air, water and soil, resulting in diverse incurable effects on humans and on the stability of the...Population explosion in the last decades together with global industrialization has caused heavy-metal contamination of air, water and soil, resulting in diverse incurable effects on humans and on the stability of the ecosystem. Non-biodegradable heavy-metals can remain in the ecosystem and the threat associated with their bioaccumulation in food chains represents one of the major environmental and health problems of present day society. Several studies were carried out to understand the ecological effects of the heavy-metal Zn in soil-plant systems. Plants often have a zinc uptake that their systems cannot handle, due to the accumulation of zinc in soils. Of the several Zn toxicity symptoms, fatal are yield reduction, stunted growth, chlorosis, reduced chlorophyll synthesis and chloroplast degradation. Vigna unguiculata is an herbaceous, annual plant in the pea family Fabaceae. In the present study, an experiment was performed to evaluate the Zn phytoextracting ability of V. unguiculata under in vitro condition. We establish that V. unguiculata can uptake a considerable amount of the heavy-metal zinc and this phytoextraction property can be utilized in long run for the cleanup of zinc contaminated soil. To the best of our knowledge, this is the first report of Zn phytoextraction ability of V. unguiculata.展开更多
文摘In recent years, proteomics has played a key role in identifying changes in protein levels in plant hosts upon infection by pathogenic organisms and in characterizing cellular and extracellular virulence and pathogenicity factors produced by pathogens. Proteomics offers a constantly evolving set of novel techniques to study all aspects of protein structure and function. Proteomics aims to find out the identity and amount of each and every protein present in a cell and actual function mediating specific cellular processes. Structural proteomics elucidates the development and application of experimental approaches to define the primary, secondary and tertiary structures of proteins, while functional proteomics refers to the development and application of global (proteome wide or system-wide) experimental approaches to assess protein function. A detail understanding of plant defense response using successful combination of proteomic techniques and other high throughput techniques of cell biology, biochemistry as well as genomics is needed for practical application to secure and stabilize yield of many crop plants. This review starts with a brief introduction to gel- and non gel-based proteomic techniques followed by the basics of plant-pathogen interaction, the use of proteomics in recent pasts to decipher the mysteries of plant-pathogen interaction, and ends with the future prospects of this technology.
文摘Population explosion in the last decades together with global industrialization has caused heavy-metal contamination of air, water and soil, resulting in diverse incurable effects on humans and on the stability of the ecosystem. Non-biodegradable heavy-metals can remain in the ecosystem and the threat associated with their bioaccumulation in food chains represents one of the major environmental and health problems of present day society. Several studies were carried out to understand the ecological effects of the heavy-metal Zn in soil-plant systems. Plants often have a zinc uptake that their systems cannot handle, due to the accumulation of zinc in soils. Of the several Zn toxicity symptoms, fatal are yield reduction, stunted growth, chlorosis, reduced chlorophyll synthesis and chloroplast degradation. Vigna unguiculata is an herbaceous, annual plant in the pea family Fabaceae. In the present study, an experiment was performed to evaluate the Zn phytoextracting ability of V. unguiculata under in vitro condition. We establish that V. unguiculata can uptake a considerable amount of the heavy-metal zinc and this phytoextraction property can be utilized in long run for the cleanup of zinc contaminated soil. To the best of our knowledge, this is the first report of Zn phytoextraction ability of V. unguiculata.
