DNAzyme that can catalytically cleave of substrate DNA has shown to be attractive for amplified detection in biosensing events. During the catalytic process, the recycling of enzyme strand of DNAzyme is critically imp...DNAzyme that can catalytically cleave of substrate DNA has shown to be attractive for amplified detection in biosensing events. During the catalytic process, the recycling of enzyme strand of DNAzyme is critically important. In this work, a G-quadruplex-assisted enzyme strand recycling strategy was developed for amplified label-free fluorescent detection of uranyl ion(UO_2^(+2)). The DNAzyme was activated by the target UO_2^(+2) and further cleaved the substrate strand that contained the G-quadruplex sequence. The following formation of G-quadruplex helps the separation between the enzyme strand and the cleaved substrate strand, thus improving the recycle use of the enzyme strand. Such strategy allowed lablel-free detection of 0.2–200 ng/mL UO_2^(+2)via SYBR green I(SG)-based fluorescence. The detection limit(3 d) is as low as0.06 ng/mL(about 0.2 nmol/L), comparable to those obtained by ICP-MS and labeled DNAzyme. It was applied for detection of UO_2^(+2) in spiked environmental water samples with recoveries in the range of96%–103%. This biosensor, with the advantages of simplicity and high sensitivity, is an appealing tool for fast detection of UO_2(+2) in environmental water samples.展开更多
基金support from the National Natural Science Foundation of China (Nos. 21475013 and 21305009)China Postdoctoral Science Foundation (Nos. 2015M572453 and 2016T90839)
文摘DNAzyme that can catalytically cleave of substrate DNA has shown to be attractive for amplified detection in biosensing events. During the catalytic process, the recycling of enzyme strand of DNAzyme is critically important. In this work, a G-quadruplex-assisted enzyme strand recycling strategy was developed for amplified label-free fluorescent detection of uranyl ion(UO_2^(+2)). The DNAzyme was activated by the target UO_2^(+2) and further cleaved the substrate strand that contained the G-quadruplex sequence. The following formation of G-quadruplex helps the separation between the enzyme strand and the cleaved substrate strand, thus improving the recycle use of the enzyme strand. Such strategy allowed lablel-free detection of 0.2–200 ng/mL UO_2^(+2)via SYBR green I(SG)-based fluorescence. The detection limit(3 d) is as low as0.06 ng/mL(about 0.2 nmol/L), comparable to those obtained by ICP-MS and labeled DNAzyme. It was applied for detection of UO_2^(+2) in spiked environmental water samples with recoveries in the range of96%–103%. This biosensor, with the advantages of simplicity and high sensitivity, is an appealing tool for fast detection of UO_2(+2) in environmental water samples.
