Prostate cancer(PC)biomarker-citrate detection is clinically important to diagnose PC in early stages.Methylquinolinium iodide(Q)conjugated indole-phenylboronic acid(IB)was designed as a red-emissive QIB probe for the...Prostate cancer(PC)biomarker-citrate detection is clinically important to diagnose PC in early stages.Methylquinolinium iodide(Q)conjugated indole-phenylboronic acid(IB)was designed as a red-emissive QIB probe for the detection of citrate through Lewis acid-base reaction and intramolecular charge transfer(ICT)sensing mechanisms.Boronic acid acts as Lewis acid as well as citrate(Lewis base)recognition unit.The probe reacted with citrate,showing enhanced red emissions.Since the probe has excellent water solubility and great biocompatibility,practical application in biological systems is possible.Citrate was monitored precisely in the mitochondria organelle(in vitro)of living cells with a positive charge on QIB.Also,endogenous(in situ)citrate was detected quantitatively to discriminate non-cancerous and PC mice,observed strong and lower(negligible)emission intensity on non-cancerous and cancerous prostate tissues,respectively.Because,the concentration of citrate is higher in healthy prostate compared with PC prostate.Furthermore,the analysis of sliced prostate tissues can give PC-related information for clinical diagnosis to prevent and treat PC in the initial stages.Therefore,we believe that the present probe is a promising biochemical reagent in diagnosing PC.展开更多
The large-scale use of antibiotics is causing serious water pollution problems,and it is of great significance to develop new technologies to remove antibiotics from water.As an environmentally friendly and economical...The large-scale use of antibiotics is causing serious water pollution problems,and it is of great significance to develop new technologies to remove antibiotics from water.As an environmentally friendly and economical adsorption material,carbon derived from biomass is a low-cost and feasible material for removing antibiotics in sewage,but the current removal efficiencies are not high enough for large-scale practical application.In this study,poplar wood chips are used as raw material,and a magnetic biochar is prepared by co-pyrolysis of poplar wood chips and FeCl_(3)/CaCl_(2)mixed molten salt.X-ray diffraction(XRD),Fourier transform infrared spectroscopy(FTIR),N_(2)-isothermal adsorption and desorption,X-ray photoelectron spectroscopy(XPS)and scanning electron microscope(SEM)techniques showed the successful synthesis of Fe_(2)O_(3)/bioC composite.In-situ formed Fe_(2)O_(3)makes the biochar possess the characteristic of superparamagnetic,which is conducive to the recycling of biochar.Due to the etching effect of the molten salts,rough surface was formed on Fe_(2)O_(3)/bioC,result-ing in a maximum norfloxacin(NOR)adsorption ability up to 38.77 mg g^(−1)at pH 6.0.The NOR adsorption behavior on Fe_(2)O_(3)/bioC followed the pseudo second order kinetic model and the equilibrium data was best fitted the Langmuir model.In addition,the adsorption process of NOR on Fe_(2)O_(3)/bioC was thermodynamically spontaneous.The results show that this low-cost and reusable magnetic biochar has the potential for rapid and efficient removal of antibiotic from aqueous solution.展开更多
基金financially supported by the National Natural Science Foundation of China(No.22150410327)the Basic Science Research Program through the National Research Foundation of Korea(NRF)funded by the Ministry of Science,ICT&Future Planning(No.2020R1A2C1102741).
文摘Prostate cancer(PC)biomarker-citrate detection is clinically important to diagnose PC in early stages.Methylquinolinium iodide(Q)conjugated indole-phenylboronic acid(IB)was designed as a red-emissive QIB probe for the detection of citrate through Lewis acid-base reaction and intramolecular charge transfer(ICT)sensing mechanisms.Boronic acid acts as Lewis acid as well as citrate(Lewis base)recognition unit.The probe reacted with citrate,showing enhanced red emissions.Since the probe has excellent water solubility and great biocompatibility,practical application in biological systems is possible.Citrate was monitored precisely in the mitochondria organelle(in vitro)of living cells with a positive charge on QIB.Also,endogenous(in situ)citrate was detected quantitatively to discriminate non-cancerous and PC mice,observed strong and lower(negligible)emission intensity on non-cancerous and cancerous prostate tissues,respectively.Because,the concentration of citrate is higher in healthy prostate compared with PC prostate.Furthermore,the analysis of sliced prostate tissues can give PC-related information for clinical diagnosis to prevent and treat PC in the initial stages.Therefore,we believe that the present probe is a promising biochemical reagent in diagnosing PC.
基金National Natural Science Foundation of China,Grant Number:21908242.
文摘The large-scale use of antibiotics is causing serious water pollution problems,and it is of great significance to develop new technologies to remove antibiotics from water.As an environmentally friendly and economical adsorption material,carbon derived from biomass is a low-cost and feasible material for removing antibiotics in sewage,but the current removal efficiencies are not high enough for large-scale practical application.In this study,poplar wood chips are used as raw material,and a magnetic biochar is prepared by co-pyrolysis of poplar wood chips and FeCl_(3)/CaCl_(2)mixed molten salt.X-ray diffraction(XRD),Fourier transform infrared spectroscopy(FTIR),N_(2)-isothermal adsorption and desorption,X-ray photoelectron spectroscopy(XPS)and scanning electron microscope(SEM)techniques showed the successful synthesis of Fe_(2)O_(3)/bioC composite.In-situ formed Fe_(2)O_(3)makes the biochar possess the characteristic of superparamagnetic,which is conducive to the recycling of biochar.Due to the etching effect of the molten salts,rough surface was formed on Fe_(2)O_(3)/bioC,result-ing in a maximum norfloxacin(NOR)adsorption ability up to 38.77 mg g^(−1)at pH 6.0.The NOR adsorption behavior on Fe_(2)O_(3)/bioC followed the pseudo second order kinetic model and the equilibrium data was best fitted the Langmuir model.In addition,the adsorption process of NOR on Fe_(2)O_(3)/bioC was thermodynamically spontaneous.The results show that this low-cost and reusable magnetic biochar has the potential for rapid and efficient removal of antibiotic from aqueous solution.
