In our previous papers,the classical fractional Fourier transform theory was incorporated into the quantum theoretical system using the theoretical method of quantum optics,and the calculation produced quantum mechani...In our previous papers,the classical fractional Fourier transform theory was incorporated into the quantum theoretical system using the theoretical method of quantum optics,and the calculation produced quantum mechanical operators corresponding to the generation of fractional Fourier transform.The core function of the coordinate-momentum exchange operators in the addition law of fractional Fourier transform was analyzed too.In this paper,the bivariate operator Hermite polynomial theory and the technique of integration within an ordered product of operators(IWOP)are used to establish the entanglement fractional Fourier transform theory to the extent of quantum.A new function generating formula and an operator for generating quantum entangled fractional Fourier transform are obtained using the fractional Fourier transform relationship in a pair of conjugated entangled state representations.展开更多
The extracellular matrix surrounding oligodendrocytes plays an important role during myelination and remyelination in the brain.In many cases,the microenvironment surrounding demyelination lesions contains inhibitory ...The extracellular matrix surrounding oligodendrocytes plays an important role during myelination and remyelination in the brain.In many cases,the microenvironment surrounding demyelination lesions contains inhibitory molecules,which lead to repair failure.Accordingly,blocking the activity of these inhibitory factors in the extracellular matrix should lead to more successful remyelination.In the central nervous system,oligodendrocytes form the myelin sheath.We performed primary cell culture and found that a natural increase in fibronectin promoted the proliferation of oligodendrocyte progenitors during the initial stage of remyelination while inhibiting oligodendrocyte differentiation.Poly-L-ornithine blocked these inhibitory effects without compromising fibronectin’s pro-proliferation function.Experiments showed that poly-L-ornithine activated the Erk1/2 signaling pathway that is necessary in the early stages of differentiation,as well as PI3K signaling pathways that are needed in the mid-late stages.When poly-L-ornithine was tested in a lysolecithin-induced animal model of focal demyelination,it enhanced myelin regeneration and promoted motor function recovery.These findings suggest that poly-L-ornithine has the potential to be a treatment option for clinical myelin sheath injury.展开更多
Multiple types of stem cells have been proposed for the treatment of spinal cord injury,but their comparative information remains elusive.In this study,a rat model of T10 contusion spinal cord injury was established b...Multiple types of stem cells have been proposed for the treatment of spinal cord injury,but their comparative information remains elusive.In this study,a rat model of T10 contusion spinal cord injury was established by the impactor method.Human umbilical cord-derived mesenchymal stem cells(UCMSCs)or human adipose tissue-derived mesenchymal stem cells(ADMSCs)(2.5μL/injection site,1×10^5 cells/μL)was injected on rostral and caudal of the injury segment on the ninth day after injury.Rats injected with mesenchymal stem cell culture medium were used as controls.Our results show that although transplanted UCMSCs and ADMSCs failed to differentiate into neurons or glial cells in vivo,both significantly improved motor and sensory function.After spinal cord injury,UCMSCs and ADMSCs similarly promoted spinal neuron survival and axonal regeneration,decreased glial scar and lesion cavity formation,and reduced numbers of active macrophages.BioPlex analysis of spinal samples showed a specific increase of interleukin-10 and decrease of tumor necrosis factorαin the ADMSC group,as well as a downregulation of macrophage inflammatory protein 3αin both UCMSC and ADMSC groups at 3 days after cell transplantation.Upregulation of interleukin-10 and interleukin-13 was observed in both UCMSC and ADMSC groups at 7 days after cell transplantation.Isobaric tagging for relative and absolute quantitation proteomics analyses showed that UCMSCs and ADMSCs induced changes of multiple genes related to axonal regeneration,neurotrophy,and cell apoptosis in common and specific manners.In conclusion,UCMSC and ADMSC transplants yielded quite similar contributions to motor and sensory recovery after spinal cord injury via anti-inflammation and improved axonal growth.However,there were some differences in cytokine and gene expression induced by these two types of transplanted cells.Animal experiments were approved by the Laboratory Animal Ethics Committee at Jinan University(approval No.20180228026)on February 28,2018,and the application of human stem cells was approved by the Medical Ethics Committee of Medical College of Jinan University of China(approval No.2016041303)on April 13,2016.展开更多
AIM: To investigate the mechanism of interleukin (IL)-6 secretion through blocking the IL-17A/IL-17A recepto (IL-17RA) signaling pathway with a short hairpin RNA (shRNA) in hepatic stellate cells (HSCs) in vitro . MET...AIM: To investigate the mechanism of interleukin (IL)-6 secretion through blocking the IL-17A/IL-17A recepto (IL-17RA) signaling pathway with a short hairpin RNA (shRNA) in hepatic stellate cells (HSCs) in vitro . METHODS: HSCs were derived from the livers of adul male Sprague-Dawley rats. IL-6 expression was evalu ated using real-time quantitative polymerase chain reaction and enzyme linked immunosorbent assay. The phosphorylation activity of p38 mitogen activated pro tein kinases (MAPK) and extracellular regulated pro tein kinases (ERK) 1/2 upon induction by IL-17A and suppression by IL-17RA shRNA were examined using Western blotting.RESULTS: IL-6 expression induced by IL-17A was significantly increased compared to control in HSCs (P < 0.01 in a dose-dependent manner). Suppression of IL17RA using lentiviral-mediated shRNA inhibited IL-6 expression induced by IL-17A compared to group with only IL-17A treatment (1.44 ± 0.17 vs 4.07 ± 0.43, P < 0.01). IL-17A induced rapid phosphorylation of p38 MAPK and ERK1/2 after 5 min exposure, and showed the strongest levels of phosphorylation of p38 MAPK and ERK1/2 at 15 min in IL-17A-treated HSCs. IL-6 mRNA expression induced by IL-17A (100 ng/mL) for 3 h exposure was inhibited by preincubation with specific inhibitors of p38 MAPK (SB-203580) and ERK1/2 (PD-98059) compared to groups without inhibitors preincubation (1.67 ± 0.24, 2.01 ± 0.10 vs 4.08 ± 0.59, P < 0.01). Moreover, lentiviral-mediated IL-17RA shRNA 1 inhibited IL-17A-induced IL-6 mRNA expression compared to random shRNA in HSCs (1.44 ± 0.17 vs 3.98 ± 0.68, P < 0.01). Lentiviral-mediated IL17RA shRNA 1 inhibited phosphorylation of p38 MAPK and ERK1/2 induced by 15 min IL-17A (100 ng/mL) exposure. CONCLUSION: Down-regulation of the IL-17RA receptor by shRNA decreased IL-6 expression induced by IL-17A via p38 MAPK and ERK1/2 phosphorylation in HSCs. Suppression of IL-17RA expression may be a strategy to reduce the inflammatory response induced by IL-17A in the liver.展开更多
BACKGROUND Aggressive fibromatosis(AF),also known as desmoid tumor or desmoid-type fibromatosis,is a rare soft tissue neoplasm that can occur in almost any part of the body.Although it is a benign disease,AF is aggres...BACKGROUND Aggressive fibromatosis(AF),also known as desmoid tumor or desmoid-type fibromatosis,is a rare soft tissue neoplasm that can occur in almost any part of the body.Although it is a benign disease,AF is aggressive and infiltrative and has a high recurrence rate after surgery.Common sites for intra-abdominal AF are the small bowel mesentery,retroperitoneum,and pelvis.AF in the colon is extremely rare.CASE SUMMARY Here,we report the first case of sigmoid colon AF,which was accidentally discovered in a 27-year-old woman during laparoscopic myomectomy.Computed tomography confirmed a slightly enhanced mass in the sigmoid colon.Subsequent colonoscopy did not reveal a mass in the colonic lumen,but a suspected external compress was found in the sigmoid colon.Surgical disease involving a gastrointestinal stromal tumor was suspected.The patient underwent laparoscopic exploration,and sigmoidectomy with a negative margin was performed to excise the mass.Postoperative immunohistochemistry revealed that the mass was an AF.The patient recovered well and was recurrence-free at the 30-month follow-up without adjuvant therapy.CONCLUSION AF should be considered in the differential diagnosis of subepithelial colon masses.Radical resection alone can achieve good outcomes.展开更多
基金Project supported by the National Natural Science Foundation of China(Grant No.11775208)the Foundation for Young Talents at the College of Anhui Province,China(Grant Nos.gxyq2021210 and gxyq2019077)the Natural Science Foundation of the Anhui Higher Education Institutions of China(Grant Nos.KJ2020A0638 and 2022AH051586)。
文摘In our previous papers,the classical fractional Fourier transform theory was incorporated into the quantum theoretical system using the theoretical method of quantum optics,and the calculation produced quantum mechanical operators corresponding to the generation of fractional Fourier transform.The core function of the coordinate-momentum exchange operators in the addition law of fractional Fourier transform was analyzed too.In this paper,the bivariate operator Hermite polynomial theory and the technique of integration within an ordered product of operators(IWOP)are used to establish the entanglement fractional Fourier transform theory to the extent of quantum.A new function generating formula and an operator for generating quantum entangled fractional Fourier transform are obtained using the fractional Fourier transform relationship in a pair of conjugated entangled state representations.
基金supported by the National Nature Science Foundation of China,Nos.81371338(to HF)and 82071369(PPY)。
文摘The extracellular matrix surrounding oligodendrocytes plays an important role during myelination and remyelination in the brain.In many cases,the microenvironment surrounding demyelination lesions contains inhibitory molecules,which lead to repair failure.Accordingly,blocking the activity of these inhibitory factors in the extracellular matrix should lead to more successful remyelination.In the central nervous system,oligodendrocytes form the myelin sheath.We performed primary cell culture and found that a natural increase in fibronectin promoted the proliferation of oligodendrocyte progenitors during the initial stage of remyelination while inhibiting oligodendrocyte differentiation.Poly-L-ornithine blocked these inhibitory effects without compromising fibronectin’s pro-proliferation function.Experiments showed that poly-L-ornithine activated the Erk1/2 signaling pathway that is necessary in the early stages of differentiation,as well as PI3K signaling pathways that are needed in the mid-late stages.When poly-L-ornithine was tested in a lysolecithin-induced animal model of focal demyelination,it enhanced myelin regeneration and promoted motor function recovery.These findings suggest that poly-L-ornithine has the potential to be a treatment option for clinical myelin sheath injury.
基金supported by Guangdong grant‘Key Technologies for Treatment of Brain Disorders’,No.2018B030332001(to LBZ)Health and Medical Collaborative Innovation Major Projects of Guangzhou of China,Nos.201803040016-2(to LBZ),201604046028(to LBZ and KFS)+2 种基金Science&Technology Planning and Key Technology Innovation Projects of Guangdong Province of China,No.2014B050504006(to LBZ)Programme of Introducing Talents of Discipline to Universities of China,No.B14036(to KFS)Science and Technology Plan Project of Guangdong Province of China,No.2017B090904033(to KFS)。
文摘Multiple types of stem cells have been proposed for the treatment of spinal cord injury,but their comparative information remains elusive.In this study,a rat model of T10 contusion spinal cord injury was established by the impactor method.Human umbilical cord-derived mesenchymal stem cells(UCMSCs)or human adipose tissue-derived mesenchymal stem cells(ADMSCs)(2.5μL/injection site,1×10^5 cells/μL)was injected on rostral and caudal of the injury segment on the ninth day after injury.Rats injected with mesenchymal stem cell culture medium were used as controls.Our results show that although transplanted UCMSCs and ADMSCs failed to differentiate into neurons or glial cells in vivo,both significantly improved motor and sensory function.After spinal cord injury,UCMSCs and ADMSCs similarly promoted spinal neuron survival and axonal regeneration,decreased glial scar and lesion cavity formation,and reduced numbers of active macrophages.BioPlex analysis of spinal samples showed a specific increase of interleukin-10 and decrease of tumor necrosis factorαin the ADMSC group,as well as a downregulation of macrophage inflammatory protein 3αin both UCMSC and ADMSC groups at 3 days after cell transplantation.Upregulation of interleukin-10 and interleukin-13 was observed in both UCMSC and ADMSC groups at 7 days after cell transplantation.Isobaric tagging for relative and absolute quantitation proteomics analyses showed that UCMSCs and ADMSCs induced changes of multiple genes related to axonal regeneration,neurotrophy,and cell apoptosis in common and specific manners.In conclusion,UCMSC and ADMSC transplants yielded quite similar contributions to motor and sensory recovery after spinal cord injury via anti-inflammation and improved axonal growth.However,there were some differences in cytokine and gene expression induced by these two types of transplanted cells.Animal experiments were approved by the Laboratory Animal Ethics Committee at Jinan University(approval No.20180228026)on February 28,2018,and the application of human stem cells was approved by the Medical Ethics Committee of Medical College of Jinan University of China(approval No.2016041303)on April 13,2016.
基金Supported by The Zhejiang Extremely Key Subject of SurgeryThe Wenzhou Key Laboratory Project in Surgery
文摘AIM: To investigate the mechanism of interleukin (IL)-6 secretion through blocking the IL-17A/IL-17A recepto (IL-17RA) signaling pathway with a short hairpin RNA (shRNA) in hepatic stellate cells (HSCs) in vitro . METHODS: HSCs were derived from the livers of adul male Sprague-Dawley rats. IL-6 expression was evalu ated using real-time quantitative polymerase chain reaction and enzyme linked immunosorbent assay. The phosphorylation activity of p38 mitogen activated pro tein kinases (MAPK) and extracellular regulated pro tein kinases (ERK) 1/2 upon induction by IL-17A and suppression by IL-17RA shRNA were examined using Western blotting.RESULTS: IL-6 expression induced by IL-17A was significantly increased compared to control in HSCs (P < 0.01 in a dose-dependent manner). Suppression of IL17RA using lentiviral-mediated shRNA inhibited IL-6 expression induced by IL-17A compared to group with only IL-17A treatment (1.44 ± 0.17 vs 4.07 ± 0.43, P < 0.01). IL-17A induced rapid phosphorylation of p38 MAPK and ERK1/2 after 5 min exposure, and showed the strongest levels of phosphorylation of p38 MAPK and ERK1/2 at 15 min in IL-17A-treated HSCs. IL-6 mRNA expression induced by IL-17A (100 ng/mL) for 3 h exposure was inhibited by preincubation with specific inhibitors of p38 MAPK (SB-203580) and ERK1/2 (PD-98059) compared to groups without inhibitors preincubation (1.67 ± 0.24, 2.01 ± 0.10 vs 4.08 ± 0.59, P < 0.01). Moreover, lentiviral-mediated IL-17RA shRNA 1 inhibited IL-17A-induced IL-6 mRNA expression compared to random shRNA in HSCs (1.44 ± 0.17 vs 3.98 ± 0.68, P < 0.01). Lentiviral-mediated IL17RA shRNA 1 inhibited phosphorylation of p38 MAPK and ERK1/2 induced by 15 min IL-17A (100 ng/mL) exposure. CONCLUSION: Down-regulation of the IL-17RA receptor by shRNA decreased IL-6 expression induced by IL-17A via p38 MAPK and ERK1/2 phosphorylation in HSCs. Suppression of IL-17RA expression may be a strategy to reduce the inflammatory response induced by IL-17A in the liver.
基金Supported by Hangzhou Health Science and Technology Program,No.A20210271.
文摘BACKGROUND Aggressive fibromatosis(AF),also known as desmoid tumor or desmoid-type fibromatosis,is a rare soft tissue neoplasm that can occur in almost any part of the body.Although it is a benign disease,AF is aggressive and infiltrative and has a high recurrence rate after surgery.Common sites for intra-abdominal AF are the small bowel mesentery,retroperitoneum,and pelvis.AF in the colon is extremely rare.CASE SUMMARY Here,we report the first case of sigmoid colon AF,which was accidentally discovered in a 27-year-old woman during laparoscopic myomectomy.Computed tomography confirmed a slightly enhanced mass in the sigmoid colon.Subsequent colonoscopy did not reveal a mass in the colonic lumen,but a suspected external compress was found in the sigmoid colon.Surgical disease involving a gastrointestinal stromal tumor was suspected.The patient underwent laparoscopic exploration,and sigmoidectomy with a negative margin was performed to excise the mass.Postoperative immunohistochemistry revealed that the mass was an AF.The patient recovered well and was recurrence-free at the 30-month follow-up without adjuvant therapy.CONCLUSION AF should be considered in the differential diagnosis of subepithelial colon masses.Radical resection alone can achieve good outcomes.
基金supported by the Innovative Research Team of Higher Education of Heilongjiang Province(No.2010td11)the National Natural Science Foundation of China(No.31000801)+1 种基金the National Key Technology R&D Program of China during the 12th Five-Year Plan Period(No.2013BAD18B06)the 2009 Doctoral Science Research of Northeast Agricultural University,China