In this study, forty celery cultivars introduced from different regions of China were used as experimental materials to verify and analyze the practicability of RAPD technology in the identification of celery cuhivars...In this study, forty celery cultivars introduced from different regions of China were used as experimental materials to verify and analyze the practicability of RAPD technology in the identification of celery cuhivars. The results showed that RAPD technology could distinguish accurately the genetic relationship among various celery cultivars as a simple and ideal DNA molecular marker technology suitable for genetic relationship analysis. To be specific, 13 RAPD primers with clear amplified bands and significantly different number of amplified bands were screened, which provided basis for the identification of genetic relationship among different celery cultivars.展开更多
The seed, leaf and root parts of three turnip ( Brassica rapa L.) cultivars were analyzed for glucosinolates. The component and concentration of glucosinolates in the three cultivars were also analyzed at different ...The seed, leaf and root parts of three turnip ( Brassica rapa L.) cultivars were analyzed for glucosinolates. The component and concentration of glucosinolates in the three cultivars were also analyzed at different growing stages. Eight kinds of glucosinolates were tested in turnip. They were PRO, NAP, 4OH, GBN, GBC, NAS, 4ME and NEO, respectively. The contents and kinds of glucosinolates varied at different growing stages. The content of NAS was the highest in the seed, GBC and NEO contents were the highest in the seedling, GBN and NEO contents were the highest in the leaf, while the highest NAS content was in the root.展开更多
文摘In this study, forty celery cultivars introduced from different regions of China were used as experimental materials to verify and analyze the practicability of RAPD technology in the identification of celery cuhivars. The results showed that RAPD technology could distinguish accurately the genetic relationship among various celery cultivars as a simple and ideal DNA molecular marker technology suitable for genetic relationship analysis. To be specific, 13 RAPD primers with clear amplified bands and significantly different number of amplified bands were screened, which provided basis for the identification of genetic relationship among different celery cultivars.
基金Supported by Special Fund for the Scientific and Technological Innovation Capacity Construction in Beijing Academy of Agriculture and Forestry(KJCX20150201,KJCX20170102-21)
文摘The seed, leaf and root parts of three turnip ( Brassica rapa L.) cultivars were analyzed for glucosinolates. The component and concentration of glucosinolates in the three cultivars were also analyzed at different growing stages. Eight kinds of glucosinolates were tested in turnip. They were PRO, NAP, 4OH, GBN, GBC, NAS, 4ME and NEO, respectively. The contents and kinds of glucosinolates varied at different growing stages. The content of NAS was the highest in the seed, GBC and NEO contents were the highest in the seedling, GBN and NEO contents were the highest in the leaf, while the highest NAS content was in the root.