The role of plant tyrosyl-DNA phosphodiesteritdase 1a in genome stability is studied using a Medicago Etruncatula Mt Tdp1a-depleted line. Lack of Mt Tdp1 a results e in a 39% reduction of methylated cytosines as compa...The role of plant tyrosyl-DNA phosphodiesteritdase 1a in genome stability is studied using a Medicago Etruncatula Mt Tdp1a-depleted line. Lack of Mt Tdp1 a results e in a 39% reduction of methylated cytosines as compared thto control. RNA-Seq analyses revealed that 11 DNA transto posons and 22 retrotransposons were differentially exrpressed in the Tdp1a-2a line. Among them all, DNA etttransposons（Mu DR, h AT, DNA3-11_Mad） and seven retroetransposons（LTR（Long Terminal Repeat）/Gipsy, LTR/Copia,LLTR and Non LTR/L1） were down-regulated, while the15 retrotransposons were up regulated. Results suggest that the occurrence of stress-responsive cis-elements as well as changes in the methylation pattern at the LTR promoters might be responsible for the enhanced retrotransposon transcription.展开更多
Viruses are the most abundant biological entities on Earth and the major carriers of disease and mortality as well as the drivers of global processes.How plant viruses potentially influence global processes is an emer...Viruses are the most abundant biological entities on Earth and the major carriers of disease and mortality as well as the drivers of global processes.How plant viruses potentially influence global processes is an emergent issue,particularly for their putative implications in applications.Upon infection and spread,plant viruses are capable of reprogramming host gene expression via RNA silencing by releasing viral suppressors of RNA silencing,viral small interfering RNAs(vsiRNAs),and by inducing the production of viral-associated small interfering RNAs(va-siRNAs).Recently,studies on various crop-virus pathosystems suggest that RNA silencing suppressors(RSSs)。展开更多
基金supported by Agricultural Research Council(C.R.A.),University of Pavia and by SaVeGraINPuglia-Progetto Integrato per la Biodiversita PSR Regione Puglia FEASR 2007-2013 Re(CE)1698/2005
文摘The role of plant tyrosyl-DNA phosphodiesteritdase 1a in genome stability is studied using a Medicago Etruncatula Mt Tdp1a-depleted line. Lack of Mt Tdp1 a results e in a 39% reduction of methylated cytosines as compared thto control. RNA-Seq analyses revealed that 11 DNA transto posons and 22 retrotransposons were differentially exrpressed in the Tdp1a-2a line. Among them all, DNA etttransposons（Mu DR, h AT, DNA3-11_Mad） and seven retroetransposons（LTR（Long Terminal Repeat）/Gipsy, LTR/Copia,LLTR and Non LTR/L1） were down-regulated, while the15 retrotransposons were up regulated. Results suggest that the occurrence of stress-responsive cis-elements as well as changes in the methylation pattern at the LTR promoters might be responsible for the enhanced retrotransposon transcription.
基金The authors are funded by Italian MIUR Progetto NUTR-AGE(FOE-2019,DSBAD004.271)to DiSBA of the National Research Council.
文摘Viruses are the most abundant biological entities on Earth and the major carriers of disease and mortality as well as the drivers of global processes.How plant viruses potentially influence global processes is an emergent issue,particularly for their putative implications in applications.Upon infection and spread,plant viruses are capable of reprogramming host gene expression via RNA silencing by releasing viral suppressors of RNA silencing,viral small interfering RNAs(vsiRNAs),and by inducing the production of viral-associated small interfering RNAs(va-siRNAs).Recently,studies on various crop-virus pathosystems suggest that RNA silencing suppressors(RSSs)。
