Objective:Long-term survivors(LS)of non-small cell lung cancer(NSCLC)without driver alterations,displaying an overall survival(OS)of more than 3 years,comprise around 10%of cases in several series treated with chemoth...Objective:Long-term survivors(LS)of non-small cell lung cancer(NSCLC)without driver alterations,displaying an overall survival(OS)of more than 3 years,comprise around 10%of cases in several series treated with chemotherapy.There are classical prognosis factors for these cases[stage,Eastern Cooperative Oncology Group(ECOG),etc.],but more data are required in the literature.In this multi-center study,we focused on LS of advanced NSCLC with OS above 36 months to perform a clinical-pathological and molecular characterization.Methods:In the first step,we conducted a clinical-pathological characterization of the patients.Afterwards,we carried out a genetic analysis by comparing LS to a sample of short-term survivors(SS)(with an OS less than 9 months).We initially used whole-genome RNA-seq to identify differentiating profiles of LS and SS,and later confirmed these with reverse transcription-polymerase chain reaction(RT-PCR)for the rest of the samples.Results:A total of 94 patients were included,who were mainly men,former smokers,having adenocarcinoma(AC)-type NSCLC with an ECOG of 0-1.We obtained an initial differential transcriptome expression,displaying 5 over-and 33 under-expressed genes involved in different pathways:namely,the secretin receptor,surfactant protein,trefoil factor 1(T FF1),serpin,Ca-channels,and Tolllike receptor(TLRs)families.Finally,RT-PCR analysis of 40(20 LS/20 SS)samples confirmed that four genes(surfactant proteins and SFTP)were significantly down-regulated in SS compared to LS by using an analysis of covariance(ANCOVA)model:SFTPA1(P=0.023),SFTPA2(P=0.027),SFTPB{P=0.02),and SFT PC(P=0.047).Conclusions:We present a sequential genetic analysis of a sample of NSCLCLS with no driver alterations,obtaining a differential RNA-seq/RT-PCR profile showing an abnormal expression of SF genes.展开更多
Purpose The purpose of the study was to investigate the acute effect of a beginner martial art class and aerobic exercise on executive function(EF)in college-aged young adults.There is overwhelming evidence that demon...Purpose The purpose of the study was to investigate the acute effect of a beginner martial art class and aerobic exercise on executive function(EF)in college-aged young adults.There is overwhelming evidence that demonstrates acute as well as long-term aerobic exercise improves EF.Nevertheless,there is limited research comparing externally paced exercise(EPE)to self-paced exercise(SPE)such as walking on improving EF.EPE requires greater cortical demand than SPE to execute a motor plan.Methods Eight men and eight women,aged 24.2±2.8 years,participated in a Repeated Measures Crossover Design.Pre-and post-testing of EF with the Stroop and Tower of London(ToL)and stress level were measured after each of the two 1-h conditions:the SPE consisted of a walk(aerobic exercise)and the EPE was a beginner martial art class.Results There were significant main effects for the martial art class for the Stroop’s mean reaction time for congruent trials(P=0.01)with a large-effect size.The mean reaction time for incongruent trials was significant(P=0.05)with a medium-effect size.The ToL’s mean solution time(P=0.003)and mean execution time(P=0.002)were also significant with large-effect sizes.Stress levels were not significantly improved following either condition.Conclusion The martial art class significantly improved all the major domains of EF,while aerobic exercise of a similar intensity did not demonstrate any measured significant changes.The physiological benefits of physical exercise are well documented;however,the cognitive enhancing capability of EPE should also be appreciated given the results of this study.展开更多
基金the following groups for aiding in the creation of this study:all the patients and their families for permitting the review of all the information included in this study,the“day hospital”workers from all the hospitals involved,the Carlos III Health Institute,the IMDEA Research Institute on Food 8c Health Sciences,the Spanish Ministry of Science(Plan Nacional I+D+i AGL2016-76736-C3)the Regional Government of Community of Madrid(S2018/BAA-4343)the Ramon Areces Foundation,the EU Structural Funds and the AECC(Spanish Association Against Cancer).Thanks to Scribendi editing and proofreading services for final manuscript review.
文摘Objective:Long-term survivors(LS)of non-small cell lung cancer(NSCLC)without driver alterations,displaying an overall survival(OS)of more than 3 years,comprise around 10%of cases in several series treated with chemotherapy.There are classical prognosis factors for these cases[stage,Eastern Cooperative Oncology Group(ECOG),etc.],but more data are required in the literature.In this multi-center study,we focused on LS of advanced NSCLC with OS above 36 months to perform a clinical-pathological and molecular characterization.Methods:In the first step,we conducted a clinical-pathological characterization of the patients.Afterwards,we carried out a genetic analysis by comparing LS to a sample of short-term survivors(SS)(with an OS less than 9 months).We initially used whole-genome RNA-seq to identify differentiating profiles of LS and SS,and later confirmed these with reverse transcription-polymerase chain reaction(RT-PCR)for the rest of the samples.Results:A total of 94 patients were included,who were mainly men,former smokers,having adenocarcinoma(AC)-type NSCLC with an ECOG of 0-1.We obtained an initial differential transcriptome expression,displaying 5 over-and 33 under-expressed genes involved in different pathways:namely,the secretin receptor,surfactant protein,trefoil factor 1(T FF1),serpin,Ca-channels,and Tolllike receptor(TLRs)families.Finally,RT-PCR analysis of 40(20 LS/20 SS)samples confirmed that four genes(surfactant proteins and SFTP)were significantly down-regulated in SS compared to LS by using an analysis of covariance(ANCOVA)model:SFTPA1(P=0.023),SFTPA2(P=0.027),SFTPB{P=0.02),and SFT PC(P=0.047).Conclusions:We present a sequential genetic analysis of a sample of NSCLCLS with no driver alterations,obtaining a differential RNA-seq/RT-PCR profile showing an abnormal expression of SF genes.
文摘Purpose The purpose of the study was to investigate the acute effect of a beginner martial art class and aerobic exercise on executive function(EF)in college-aged young adults.There is overwhelming evidence that demonstrates acute as well as long-term aerobic exercise improves EF.Nevertheless,there is limited research comparing externally paced exercise(EPE)to self-paced exercise(SPE)such as walking on improving EF.EPE requires greater cortical demand than SPE to execute a motor plan.Methods Eight men and eight women,aged 24.2±2.8 years,participated in a Repeated Measures Crossover Design.Pre-and post-testing of EF with the Stroop and Tower of London(ToL)and stress level were measured after each of the two 1-h conditions:the SPE consisted of a walk(aerobic exercise)and the EPE was a beginner martial art class.Results There were significant main effects for the martial art class for the Stroop’s mean reaction time for congruent trials(P=0.01)with a large-effect size.The mean reaction time for incongruent trials was significant(P=0.05)with a medium-effect size.The ToL’s mean solution time(P=0.003)and mean execution time(P=0.002)were also significant with large-effect sizes.Stress levels were not significantly improved following either condition.Conclusion The martial art class significantly improved all the major domains of EF,while aerobic exercise of a similar intensity did not demonstrate any measured significant changes.The physiological benefits of physical exercise are well documented;however,the cognitive enhancing capability of EPE should also be appreciated given the results of this study.