Sorghum[Sorghum bicolor(L.)Moench]can benefit from accelerated breeding and release of improved varieties through doubled haploid technology.The technology has been used in speeding up the breeding of other major cere...Sorghum[Sorghum bicolor(L.)Moench]can benefit from accelerated breeding and release of improved varieties through doubled haploid technology.The technology has been used in speeding up the breeding of other major cereals such as wheat,maize and rice,for which generally widely applied optimised protocols exist.A reproducible protocol for the crop,that can overcome genotype dependency and other species-specific challenges such as phenolic exudation is however lacking.This study aimed at sorghum doubled haploids production thereby contributing to the development of an improved protocol.From the 28 hybrid genotypes,both F1 registered-and experimental hybrids involved,this study successfully produced haploids from five genotypes and subsequently,four confirmed doubled-haploid lines on W14mf medium or its modification with 1.0 gl^(-1)L-proline,1.0 gl^(-1)L-asparagine and 1.0 gl^(-1)KH_(2)PO_(4).Medium 190-2Cu was used for regeneration and rooting,which occurred successfully,if the calli were transferred on to it less than 7 days after induction,and temperature was maintained at 25°C under light condition.Genotype dependency was not wholly overcome;however,sorghum’s high tillering ability and abiotic stress tolerance were observed to contribute to attainment of haploid plantlets.Spontaneous diploids producing seeds at rates of upto 80.5%were obtained,therefore eliminating the need for colchicine duplication.展开更多
Many attempts on optimization of sorghum[Sorghum bicolor(L.)Moench]tissue culture induction media have been made,but the culture system remains with some bottlenecks compared to that of other crops.This study aimed at...Many attempts on optimization of sorghum[Sorghum bicolor(L.)Moench]tissue culture induction media have been made,but the culture system remains with some bottlenecks compared to that of other crops.This study aimed at assessing the suitability of various induction media to produce embryogenic callus(yellow and friable)with high induction rates and reduced phenolic exudation.The six culture medium modifications:3 based on Murashige and Skoog(MS)medium and one each based on Chu N6,Gamborg B5 and 190-2 media respectively were applied in the culture of mature embryos from 10 sorghum genotypes.Although there was a genotype influence on the attainment of a yellow callus,friability of the callus was determined to be dependent on the culture medium and not the genotype.Half strength MS medium with 0.2 mg/l 2,4-D with 2.8 g/l Gelrite®as the gelling agent modified with 1.0 g/l KH_(2)PO_(4),1.0 g/l L-proline,1.0 g/l L-asparagine and 0.16 mg/l CuSO_(4)·5H_(2)O(type E)was found to be the most effective resulting in about 60%yellow coloured callus induction with 25%friability.Addition of CuSO_(4)·5H_(2)O,KH_(2)PO_(4),L-proline and L-asparagine significantly reduced the phenolic production.Half strength MS medium was observed to contribute to quality callus production when compared to full strength MS media modified with the compounds.The half strength MS medium was also observed to suppress phenolic production.Medium 190-2 produced the highest regeneration frequency(40%)among the 3-regeneration media tested.The results provide information on a suitable sorghum callus induction medium necessary for embryogenesis.展开更多
基金This work was supported through the Stipendium Hungaricum Scholarship to the first author,János Bolyai Research Scholarship of the Hungarian Academy of Sciences,as well as research grants(TUDFO/51757/2019-ITM and K_16-K119835).
文摘Sorghum[Sorghum bicolor(L.)Moench]can benefit from accelerated breeding and release of improved varieties through doubled haploid technology.The technology has been used in speeding up the breeding of other major cereals such as wheat,maize and rice,for which generally widely applied optimised protocols exist.A reproducible protocol for the crop,that can overcome genotype dependency and other species-specific challenges such as phenolic exudation is however lacking.This study aimed at sorghum doubled haploids production thereby contributing to the development of an improved protocol.From the 28 hybrid genotypes,both F1 registered-and experimental hybrids involved,this study successfully produced haploids from five genotypes and subsequently,four confirmed doubled-haploid lines on W14mf medium or its modification with 1.0 gl^(-1)L-proline,1.0 gl^(-1)L-asparagine and 1.0 gl^(-1)KH_(2)PO_(4).Medium 190-2Cu was used for regeneration and rooting,which occurred successfully,if the calli were transferred on to it less than 7 days after induction,and temperature was maintained at 25°C under light condition.Genotype dependency was not wholly overcome;however,sorghum’s high tillering ability and abiotic stress tolerance were observed to contribute to attainment of haploid plantlets.Spontaneous diploids producing seeds at rates of upto 80.5%were obtained,therefore eliminating the need for colchicine duplication.
基金This work was also supported by grants from the Higher Education Institutional Excellence Program of the Ministry of Human Capacities in Hungary within the framework of plant breeding and plant protection researches of Szent Istvan University(20430-3/2018/FEKUTSTRAT)2017-1.3.1-VKE-2017-0030 and the EFOP-3.6.3-VEKOP-16-2017-00008 project(co-financed by the European Union and the European Social Fund).
文摘Many attempts on optimization of sorghum[Sorghum bicolor(L.)Moench]tissue culture induction media have been made,but the culture system remains with some bottlenecks compared to that of other crops.This study aimed at assessing the suitability of various induction media to produce embryogenic callus(yellow and friable)with high induction rates and reduced phenolic exudation.The six culture medium modifications:3 based on Murashige and Skoog(MS)medium and one each based on Chu N6,Gamborg B5 and 190-2 media respectively were applied in the culture of mature embryos from 10 sorghum genotypes.Although there was a genotype influence on the attainment of a yellow callus,friability of the callus was determined to be dependent on the culture medium and not the genotype.Half strength MS medium with 0.2 mg/l 2,4-D with 2.8 g/l Gelrite®as the gelling agent modified with 1.0 g/l KH_(2)PO_(4),1.0 g/l L-proline,1.0 g/l L-asparagine and 0.16 mg/l CuSO_(4)·5H_(2)O(type E)was found to be the most effective resulting in about 60%yellow coloured callus induction with 25%friability.Addition of CuSO_(4)·5H_(2)O,KH_(2)PO_(4),L-proline and L-asparagine significantly reduced the phenolic production.Half strength MS medium was observed to contribute to quality callus production when compared to full strength MS media modified with the compounds.The half strength MS medium was also observed to suppress phenolic production.Medium 190-2 produced the highest regeneration frequency(40%)among the 3-regeneration media tested.The results provide information on a suitable sorghum callus induction medium necessary for embryogenesis.
