Objective:To investigate the effect of extraction parameters with particular interest during the microwave deacetylation process on the characteristics of chitosan produced from Penaeus monodon(P.monodon)sourced withi...Objective:To investigate the effect of extraction parameters with particular interest during the microwave deacetylation process on the characteristics of chitosan produced from Penaeus monodon(P.monodon)sourced within the coastal region of Lagos,Nigeria for applications of controlled release systems for pharmaceutical industries.Methods:Chitosan was extracted from shrimp(P.monodon)shell and evaluated as a controlled release system for curcumin.Effects of relevant processing parameters on physicochemical and functional characteristics of the extracted chitosan were assessed.The crude chitosan was purified and used to prepare controlled release formulations for curcumin via ionic gelation with tripolyphosphate.Results:Data from the study showed that increasing time and temperature during deproteinization significantly improved the removal of protein bound to the shell matrix.Also,the ratio of the weight of the deproteinized sample to the volume of HCl used for demineralization influenced the process.During microwave-assisted production of chitosan from chitin,increase in the concentration of the deacetylating medium significantly increased solubility,viscosity and degree of deacetylation,whereas increasing temperature and time during deacetylation of chitin degraded the biopolymer to give low molecular weight chitosan.Optimized extraction and purification process yielded absolutely soluble medium to low molecular weight chitosan.The encapsulation efficiency,loading capacity,percentage yield,release efficiencies in simulated gastric and intestinal fluids of curcumin loaded in the formulations of chitosan from P.monodon were compared favorably to encapsulation and release characteristics of the encapsulated curcumin in commercially available chitosan used as the reference.Conclusions:Valorization of shrimp waste into pharmaceutically graded medium molecular weight chitosan was achieved.The chitosan obtained can be used as a controlled release system for phyto-pharmaceuticals.展开更多
文摘Objective:To investigate the effect of extraction parameters with particular interest during the microwave deacetylation process on the characteristics of chitosan produced from Penaeus monodon(P.monodon)sourced within the coastal region of Lagos,Nigeria for applications of controlled release systems for pharmaceutical industries.Methods:Chitosan was extracted from shrimp(P.monodon)shell and evaluated as a controlled release system for curcumin.Effects of relevant processing parameters on physicochemical and functional characteristics of the extracted chitosan were assessed.The crude chitosan was purified and used to prepare controlled release formulations for curcumin via ionic gelation with tripolyphosphate.Results:Data from the study showed that increasing time and temperature during deproteinization significantly improved the removal of protein bound to the shell matrix.Also,the ratio of the weight of the deproteinized sample to the volume of HCl used for demineralization influenced the process.During microwave-assisted production of chitosan from chitin,increase in the concentration of the deacetylating medium significantly increased solubility,viscosity and degree of deacetylation,whereas increasing temperature and time during deacetylation of chitin degraded the biopolymer to give low molecular weight chitosan.Optimized extraction and purification process yielded absolutely soluble medium to low molecular weight chitosan.The encapsulation efficiency,loading capacity,percentage yield,release efficiencies in simulated gastric and intestinal fluids of curcumin loaded in the formulations of chitosan from P.monodon were compared favorably to encapsulation and release characteristics of the encapsulated curcumin in commercially available chitosan used as the reference.Conclusions:Valorization of shrimp waste into pharmaceutically graded medium molecular weight chitosan was achieved.The chitosan obtained can be used as a controlled release system for phyto-pharmaceuticals.
