There are so many existing methods to obtain system reliability like re-generating point function technique, supplementary variables technique etc., but all these techniques are full of complicated calculations. Keepi...There are so many existing methods to obtain system reliability like re-generating point function technique, supplementary variables technique etc., but all these techniques are full of complicated calculations. Keeping above these facts in mind, the authors in this paper have evaluated some reliability parameters for tele-communication system by using Boolean functions technique and algebraic method. Reliability of considered tele-communication system has been evaluated by considering the fact that failures follow arbitrary time distribution. In particular, the reliability expression has also been calculated for Exponential and Weibull distributions. Further, an important reliability parameter namely M.T.T.F. (mean time to failure) has also been calculated. A numerical example with graphical illustrations has been appended at the end to highlight the important results and practical utility of the model.展开更多
Extracellular cellulase free xylanase from Pseudomonas sp. XPB-6 was purified to homogeneity with 2.15 fold of purity and 60.31% yield, respectively by ammonium sulfate precipitation and DEAE ion exchange chromatograp...Extracellular cellulase free xylanase from Pseudomonas sp. XPB-6 was purified to homogeneity with 2.15 fold of purity and 60.31% yield, respectively by ammonium sulfate precipitation and DEAE ion exchange chromatography. The protein was found to be a homotrimer consisting of three subunits of 41 kDa each and the native molecular weight of 123 kDa. The pH and temperature optimum for enzyme were 7.5 in 100 mM sodium phosphate buffer at 60°C and was stable to a high and broader range of pH and to a temperature up to 75°C. Kinetic experiments at 60°C gave Vmax and Km values of 144.92 U/mg and 0.60 mg·ml-1 respectively for birch wood xylan. The enzyme had no apparent requirement for cofactors, and its activity was strongly inhibited 1 mM Hg2+ and Cu2+ while it was slightly inactivated by 1 mM Mg2+ and Pb2+. Shelf life of xylanase of Pseudomonas sp. XPB-6 was studied both at room temperature and 4°C. The enzyme was stable both at room temperature and at 4°C for 90 and 28 days respectively. The half-life of the enzyme was approximately 40 days at room temperature.展开更多
文摘There are so many existing methods to obtain system reliability like re-generating point function technique, supplementary variables technique etc., but all these techniques are full of complicated calculations. Keeping above these facts in mind, the authors in this paper have evaluated some reliability parameters for tele-communication system by using Boolean functions technique and algebraic method. Reliability of considered tele-communication system has been evaluated by considering the fact that failures follow arbitrary time distribution. In particular, the reliability expression has also been calculated for Exponential and Weibull distributions. Further, an important reliability parameter namely M.T.T.F. (mean time to failure) has also been calculated. A numerical example with graphical illustrations has been appended at the end to highlight the important results and practical utility of the model.
文摘Extracellular cellulase free xylanase from Pseudomonas sp. XPB-6 was purified to homogeneity with 2.15 fold of purity and 60.31% yield, respectively by ammonium sulfate precipitation and DEAE ion exchange chromatography. The protein was found to be a homotrimer consisting of three subunits of 41 kDa each and the native molecular weight of 123 kDa. The pH and temperature optimum for enzyme were 7.5 in 100 mM sodium phosphate buffer at 60°C and was stable to a high and broader range of pH and to a temperature up to 75°C. Kinetic experiments at 60°C gave Vmax and Km values of 144.92 U/mg and 0.60 mg·ml-1 respectively for birch wood xylan. The enzyme had no apparent requirement for cofactors, and its activity was strongly inhibited 1 mM Hg2+ and Cu2+ while it was slightly inactivated by 1 mM Mg2+ and Pb2+. Shelf life of xylanase of Pseudomonas sp. XPB-6 was studied both at room temperature and 4°C. The enzyme was stable both at room temperature and at 4°C for 90 and 28 days respectively. The half-life of the enzyme was approximately 40 days at room temperature.
