目的:观察强心2号方治疗慢性心力衰竭的临床疗效。方法:将81例慢性心力衰竭患者按照随机数字表法分为治疗组39例和对照组42例。对照组在治疗原发病基础上口服马来酸依那普利叶酸片、倍他乐克片,治疗组在对照组的基础上加服强心2号方。...目的:观察强心2号方治疗慢性心力衰竭的临床疗效。方法:将81例慢性心力衰竭患者按照随机数字表法分为治疗组39例和对照组42例。对照组在治疗原发病基础上口服马来酸依那普利叶酸片、倍他乐克片,治疗组在对照组的基础上加服强心2号方。比较两组患者中医证候疗效、心功能疗效及治疗前后中医证候积分、左室功能、心率变异[记录24 h内正常R-R间期的标准差(standard deviation of normol R-R intervals,SDNN)、24 h内连续每5 min正常R-R间期的标准差(standard deviation of 5 min average normol R-R intervals,SDANN)及24 h内相邻R-R间期差值的均方根(square root of the mean of the squared differences between adjacent normal R-R intervals,rMSSD)]情况,检测两组患者治疗前后N末端脑钠肽前体(N-terminal pro brain natriuretic peptide,NT-proBNP)水平。结果:中医证候疗效:对照组有效率为31.0%,治疗组有效率为89.7%,两组患者有效率比较,差异具有统计学意义(P<0.05)。心功能疗效:对照组有效率为69.0%,治疗组有效率为82.1%,两组患者有效率比较,差异无统计学意义(P>0.05)。两组患者治疗后中医证候积分及NT-proBNP低于本组治疗前,且治疗组治疗后低于对照组治疗后,差异具有统计学意义(P<0.05)。两组患者治疗后心功能级别改善例数多于治疗前,且治疗组治疗后多于对照组治疗后,差异具有统计学意义(P<0.05)。治疗组治疗后左室功能各指标均低于本组治疗前及对照组治疗后,差异具有统计学意义(P<0.05);对照组治疗后左室功能各指标低于本组治疗前,仅LVEDD与治疗前比较,差异具有统计学意义(P<0.05)。治疗组治疗后SDNN、SDANN、rMSSD均高于本组治疗前及对照组治疗后,差异具有统计学意义(P<0.05);对照组治疗后心率变异各指标高于本组治疗前,仅rMSSD与治疗前比较,差异具有统计学意义(P<0.05)。结论:强心2号方治疗慢性心力衰竭,可明显改善患者临床症状,有效抑制心室重构,调节神经内分泌水平。展开更多
Ubiquitin E2 enzyme variant （UEV） proteins are similar to ubiquitin-conjugating enzyme （E2） in both sequence and structure, but the lack of a catalytic cysteine residue renders them incapable of forming a thiolest...Ubiquitin E2 enzyme variant （UEV） proteins are similar to ubiquitin-conjugating enzyme （E2） in both sequence and structure, but the lack of a catalytic cysteine residue renders them incapable of forming a thiolester linkage with ubiquitin. While the functional roles of several UEVs have been defined in yeast and animal systems, Arabidopsis COP10, a photomorphogenesis repressor, is the only UEV characterized in plants. Phylogenetic analysis revealed that the eight Arabidopsis UEV genes belong to three subfamilies. The expresslon of those genes is supported by either the presence of ESTs or RT-PCR analysis. We also characterized the other members of the COP10 subfamily, UEV2. Semi-quantitative RT-PCR analysis indicated that the UEV2 transcripts can be detected in most organs of Arabidopsis. Analysis of UEV2：：GUS transgenic lines also showed its ubiquitous expression in nearly all the developmental stages of Arabidopsis. Transient expression analysis indicated that the sGFP-UEV2 fusion protein can localize to both the cytoplasm and nucleus. A T-DNA insertion mutant, uev2-1, which abolished the transcription of UEV2, displays no visible phenotype. Further, the cop10-4 uev2-1 double mutant exhibits the same phenotype as the cop10-4 mutant in darkness. UEV2 is therefore not functionally redundant with COP10.展开更多
文摘目的:观察强心2号方治疗慢性心力衰竭的临床疗效。方法:将81例慢性心力衰竭患者按照随机数字表法分为治疗组39例和对照组42例。对照组在治疗原发病基础上口服马来酸依那普利叶酸片、倍他乐克片,治疗组在对照组的基础上加服强心2号方。比较两组患者中医证候疗效、心功能疗效及治疗前后中医证候积分、左室功能、心率变异[记录24 h内正常R-R间期的标准差(standard deviation of normol R-R intervals,SDNN)、24 h内连续每5 min正常R-R间期的标准差(standard deviation of 5 min average normol R-R intervals,SDANN)及24 h内相邻R-R间期差值的均方根(square root of the mean of the squared differences between adjacent normal R-R intervals,rMSSD)]情况,检测两组患者治疗前后N末端脑钠肽前体(N-terminal pro brain natriuretic peptide,NT-proBNP)水平。结果:中医证候疗效:对照组有效率为31.0%,治疗组有效率为89.7%,两组患者有效率比较,差异具有统计学意义(P<0.05)。心功能疗效:对照组有效率为69.0%,治疗组有效率为82.1%,两组患者有效率比较,差异无统计学意义(P>0.05)。两组患者治疗后中医证候积分及NT-proBNP低于本组治疗前,且治疗组治疗后低于对照组治疗后,差异具有统计学意义(P<0.05)。两组患者治疗后心功能级别改善例数多于治疗前,且治疗组治疗后多于对照组治疗后,差异具有统计学意义(P<0.05)。治疗组治疗后左室功能各指标均低于本组治疗前及对照组治疗后,差异具有统计学意义(P<0.05);对照组治疗后左室功能各指标低于本组治疗前,仅LVEDD与治疗前比较,差异具有统计学意义(P<0.05)。治疗组治疗后SDNN、SDANN、rMSSD均高于本组治疗前及对照组治疗后,差异具有统计学意义(P<0.05);对照组治疗后心率变异各指标高于本组治疗前,仅rMSSD与治疗前比较,差异具有统计学意义(P<0.05)。结论:强心2号方治疗慢性心力衰竭,可明显改善患者临床症状,有效抑制心室重构,调节神经内分泌水平。
基金Publication of this paper is supported by the National Natural Science Foundation of China （30624808） and Science Publication Foundation of the Chinese Academy of Sciences.
文摘Ubiquitin E2 enzyme variant （UEV） proteins are similar to ubiquitin-conjugating enzyme （E2） in both sequence and structure, but the lack of a catalytic cysteine residue renders them incapable of forming a thiolester linkage with ubiquitin. While the functional roles of several UEVs have been defined in yeast and animal systems, Arabidopsis COP10, a photomorphogenesis repressor, is the only UEV characterized in plants. Phylogenetic analysis revealed that the eight Arabidopsis UEV genes belong to three subfamilies. The expresslon of those genes is supported by either the presence of ESTs or RT-PCR analysis. We also characterized the other members of the COP10 subfamily, UEV2. Semi-quantitative RT-PCR analysis indicated that the UEV2 transcripts can be detected in most organs of Arabidopsis. Analysis of UEV2：：GUS transgenic lines also showed its ubiquitous expression in nearly all the developmental stages of Arabidopsis. Transient expression analysis indicated that the sGFP-UEV2 fusion protein can localize to both the cytoplasm and nucleus. A T-DNA insertion mutant, uev2-1, which abolished the transcription of UEV2, displays no visible phenotype. Further, the cop10-4 uev2-1 double mutant exhibits the same phenotype as the cop10-4 mutant in darkness. UEV2 is therefore not functionally redundant with COP10.
