目的:分析阿柏西普和雷珠单抗对糖尿病性黄斑水肿治疗的疗效。方法:前瞻性研究。选择2019-11/2020-02于邢台市人民医院眼科首次就诊的糖尿病性黄斑水肿(DME)的患者纳入研究,随机按治疗方式分为阿柏西普组与雷珠单抗组,采用3+PRN(pro re ...目的:分析阿柏西普和雷珠单抗对糖尿病性黄斑水肿治疗的疗效。方法:前瞻性研究。选择2019-11/2020-02于邢台市人民医院眼科首次就诊的糖尿病性黄斑水肿(DME)的患者纳入研究,随机按治疗方式分为阿柏西普组与雷珠单抗组,采用3+PRN(pro re nata)的治疗方案,两次注射时间间隔至少4wk,所有患者均先注射3次,随访时根据患者的最佳矫正视力(BCVA)及黄斑部中心凹视网膜厚度(CFT)的大小决定是否再次注射,患者均完成12mo随访,记录治疗前后两组患者的BCVA、CFT、眼压以及注射次数的变化。结果:两组患者的BCVA、CFT在术前及术后随访中均有差异(P<0.05),两组间BCVA及CFT随访期间均无差异(P>0.05)。随访结束时,阿柏西普组平均注射次数为6.094±0.689次,雷珠单抗组为7.231±0.652次,比较无差异(t=-6.403,P<0.05)。所有患者均未出现眼部并发症以及全身不良反应。结论:与玻璃体腔注射雷珠单抗比较,注射阿柏西普注射液治疗DME能取得相似的治疗效果,且注射次数更少。展开更多
An approach for distinguishing two types of positional isomers of dimeric shikonin and its analogs was explored with ^(4)JC,H long-range correlation by prolonging the acquisition time at ^(2,3)JC,H values of 2.0 and 8...An approach for distinguishing two types of positional isomers of dimeric shikonin and its analogs was explored with ^(4)JC,H long-range correlation by prolonging the acquisition time at ^(2,3)JC,H values of 2.0 and 8.0Hz.Furthermore,the ^(1)H(proton)nuclear magnetic resonance(NMR)pattern of phenolic hydroxyl protons was developed as a“diagnosis signal”to ascertain the relative location of each side chain in DMSO-d_(6) at sample concentrations of 0.022-0.034 mol/L.The chemical shift differences of 0.6ppm between OH-5' and OH-1 and between OH-8'and OH-4 are assigned to Type A and Type B,respectively.All reported ambiguous structures were corrected by this pattern.Additionally,the steric structures of isolated compounds were elucidated by quantum chemical calculations of electronic circular dichroism(ECD)spectra.展开更多
Arnequinol A(1),featuring an unprecedented 6/6/3 tricyclic carbon skeleton fused with a heptatomic oxo-bridge,together with arnequinone A(2)bearing a highly conjugated methyl-shifting benzogeijerene skeleton,were isol...Arnequinol A(1),featuring an unprecedented 6/6/3 tricyclic carbon skeleton fused with a heptatomic oxo-bridge,together with arnequinone A(2)bearing a highly conjugated methyl-shifting benzogeijerene skeleton,were isolated from Arnebia euchroma.Their structures were elucidated by extensive spectro-scopic methods and quantum chemical calculations of the 13 C nuclear magnetic resonance(NMR)data and electronic circular dichroism(ECD)spectra.The plausible biosynthetic pathways for 1 and 2 were presented.In in vitro test,compound 2 showed potent neuroprotective activity against serum-deprivation induced PC12 cell damage at a concentration of 10μmol/L.展开更多
Four new glycosides including a eudesmane-type sesquiterpenoid(1), a guaiane-type sesquiterpenoid(2), and two C_(14)-polyacetylenes(3, 4) were isolated from the rhizomes of Atractylodes lancea. Their structure...Four new glycosides including a eudesmane-type sesquiterpenoid(1), a guaiane-type sesquiterpenoid(2), and two C_(14)-polyacetylenes(3, 4) were isolated from the rhizomes of Atractylodes lancea. Their structures were elucidated by means of spectroscopic and spectrometric analyses(UV, IR, 1D and 2D NMR, and HR-ESIMS). The absolute configurations of their aglycones were established based on the experimental and calculated electronic circular dichroism(ECD), whereas those of monosaccharide moieties were determined by the GC method after chiral derivatization. Compound 4 showed weak anti-inflammatory effects on the LPS-induced NO production in microglia BV2 cells at a concentration of10μmmolL^(-1).展开更多
Main observation and conclusion Five novel pterocarpan derivatives(1—5)and three known pterocarpans were isolated from the roots of Sophora flavescens Ait.Their structures were elucidated based on extensive spectrosc...Main observation and conclusion Five novel pterocarpan derivatives(1—5)and three known pterocarpans were isolated from the roots of Sophora flavescens Ait.Their structures were elucidated based on extensive spectroscopic data(UV,IR,1D and 2D NMR,and HR-ESI-MS).Notably,compounds 1—5 were the first reported natural products,which possess unique pterocarpan-glucose-long chain aliphatic acid structures.The kinds of aliphatic acids were determined by comparison with authentic aliphatic acids using HPLC-ELSD analysis after acid hydrolysis.The absolute configurations of 1—5 were confirmed by analysis of their ECD spectra.All compounds were evaluated against lipopolysaccharide(LPS)-induced TNF-αproduction in RAW264.7 cells,and compound 1 showed significant inhibitory activity with inhibition rate of 86.22%,IC_(50)=0.79μmol/L(dexamethasone as positive control,inhibition rate of 71.38%).展开更多
文摘目的:分析阿柏西普和雷珠单抗对糖尿病性黄斑水肿治疗的疗效。方法:前瞻性研究。选择2019-11/2020-02于邢台市人民医院眼科首次就诊的糖尿病性黄斑水肿(DME)的患者纳入研究,随机按治疗方式分为阿柏西普组与雷珠单抗组,采用3+PRN(pro re nata)的治疗方案,两次注射时间间隔至少4wk,所有患者均先注射3次,随访时根据患者的最佳矫正视力(BCVA)及黄斑部中心凹视网膜厚度(CFT)的大小决定是否再次注射,患者均完成12mo随访,记录治疗前后两组患者的BCVA、CFT、眼压以及注射次数的变化。结果:两组患者的BCVA、CFT在术前及术后随访中均有差异(P<0.05),两组间BCVA及CFT随访期间均无差异(P>0.05)。随访结束时,阿柏西普组平均注射次数为6.094±0.689次,雷珠单抗组为7.231±0.652次,比较无差异(t=-6.403,P<0.05)。所有患者均未出现眼部并发症以及全身不良反应。结论:与玻璃体腔注射雷珠单抗比较,注射阿柏西普注射液治疗DME能取得相似的治疗效果,且注射次数更少。
基金funded by the CAMS Innovation Fund for Medical Sciences(CIFMS,No.2021-I2M-1-028).
文摘An approach for distinguishing two types of positional isomers of dimeric shikonin and its analogs was explored with ^(4)JC,H long-range correlation by prolonging the acquisition time at ^(2,3)JC,H values of 2.0 and 8.0Hz.Furthermore,the ^(1)H(proton)nuclear magnetic resonance(NMR)pattern of phenolic hydroxyl protons was developed as a“diagnosis signal”to ascertain the relative location of each side chain in DMSO-d_(6) at sample concentrations of 0.022-0.034 mol/L.The chemical shift differences of 0.6ppm between OH-5' and OH-1 and between OH-8'and OH-4 are assigned to Type A and Type B,respectively.All reported ambiguous structures were corrected by this pattern.Additionally,the steric structures of isolated compounds were elucidated by quantum chemical calculations of electronic circular dichroism(ECD)spectra.
基金supported by the CAMS Innovation Fund for Medical Sciences(No.2016-I2M-1-010,China).
文摘Arnequinol A(1),featuring an unprecedented 6/6/3 tricyclic carbon skeleton fused with a heptatomic oxo-bridge,together with arnequinone A(2)bearing a highly conjugated methyl-shifting benzogeijerene skeleton,were isolated from Arnebia euchroma.Their structures were elucidated by extensive spectro-scopic methods and quantum chemical calculations of the 13 C nuclear magnetic resonance(NMR)data and electronic circular dichroism(ECD)spectra.The plausible biosynthetic pathways for 1 and 2 were presented.In in vitro test,compound 2 showed potent neuroprotective activity against serum-deprivation induced PC12 cell damage at a concentration of 10μmol/L.
基金funded by the National Science and Technology Project of China(No.2012ZX09301002-001003)
文摘Four new glycosides including a eudesmane-type sesquiterpenoid(1), a guaiane-type sesquiterpenoid(2), and two C_(14)-polyacetylenes(3, 4) were isolated from the rhizomes of Atractylodes lancea. Their structures were elucidated by means of spectroscopic and spectrometric analyses(UV, IR, 1D and 2D NMR, and HR-ESIMS). The absolute configurations of their aglycones were established based on the experimental and calculated electronic circular dichroism(ECD), whereas those of monosaccharide moieties were determined by the GC method after chiral derivatization. Compound 4 showed weak anti-inflammatory effects on the LPS-induced NO production in microglia BV2 cells at a concentration of10μmmolL^(-1).
基金supported by the National Natural Science Foundtion of China(No.81973194)CAMS Innovation Fund for Medical Sciences(CIFMS)(2019-12M-1-005).
文摘Main observation and conclusion Five novel pterocarpan derivatives(1—5)and three known pterocarpans were isolated from the roots of Sophora flavescens Ait.Their structures were elucidated based on extensive spectroscopic data(UV,IR,1D and 2D NMR,and HR-ESI-MS).Notably,compounds 1—5 were the first reported natural products,which possess unique pterocarpan-glucose-long chain aliphatic acid structures.The kinds of aliphatic acids were determined by comparison with authentic aliphatic acids using HPLC-ELSD analysis after acid hydrolysis.The absolute configurations of 1—5 were confirmed by analysis of their ECD spectra.All compounds were evaluated against lipopolysaccharide(LPS)-induced TNF-αproduction in RAW264.7 cells,and compound 1 showed significant inhibitory activity with inhibition rate of 86.22%,IC_(50)=0.79μmol/L(dexamethasone as positive control,inhibition rate of 71.38%).