In this study,an optimization model of a single machine system integrating imperfect preventive maintenance planning and production scheduling based on game theory is proposed.The costs of the production department an...In this study,an optimization model of a single machine system integrating imperfect preventive maintenance planning and production scheduling based on game theory is proposed.The costs of the production department and the maintenance department are minimized,respectively.Two kinds of three-stage dynamic game models and a backward induction method are proposed to determine the preventive maintenance(PM)threshold.A lemma is presented to obtain the exact solution.A comprehensive numerical study is provided to illustrate the proposed maintenance model.The effectiveness is also validated by comparison with other two existed optimization models.展开更多
Gallbladder carcinoma (GBC) is a malignant tumor of the bil-iary system that is aggressive, difficult to detect early, and has a low surgical resection rate and poor prognosis. Ap-propriate in vitro growth models are ...Gallbladder carcinoma (GBC) is a malignant tumor of the bil-iary system that is aggressive, difficult to detect early, and has a low surgical resection rate and poor prognosis. Ap-propriate in vitro growth models are expected to focus on the study of the biological behavior and assess treatment effects. Nonetheless, cancer initiation, progression, and in-vasion include spatiotemporal changes and changes in the cell microenvironment intracellular communication, and in-tracellular molecules, making the development of in vitro growth models very challenging. Recent advances in bioma-terial methods and tissue engineering, particularly advances in bioprinting procedures, have paved the way for advances in the creative phase of in vitro cancer research. To date, an increasing number of cultured models of gallbladder disease have emerged, such as two-dimensional (2D) GBC growth cell cultures, three-dimensional (3D) GBC growth cell cul-tures, xenograft models, and 3D bioprinting methods. These models can serve as stronger platforms, focusing on tumor growth initiation, the association with the microenvironment, angiogenesis, motility, aggression, and infiltration. Bioprint-ed growth models can also be used for high-throughput drug screening and validation, as well as translational opportuni-ties for individual cancer therapy. This study focused on the exploration, progress, and significance of the development of GBC cultural models. We present our views on the short-comings of existing models, investigate new innovations, and plan future improvements and application possibilities for cancer models.展开更多
Olfaction,the sense of smell,is a fundamental trait crucial to many species.The olfactory bulb(OB)plays pivotal roles in processing and transmitting odor information from the environment to the brain.The cellular hete...Olfaction,the sense of smell,is a fundamental trait crucial to many species.The olfactory bulb(OB)plays pivotal roles in processing and transmitting odor information from the environment to the brain.The cellular heterogeneity of the mouse OB has been studied using single-cell RNA sequencing.However,the epigenetic landscape of the m OB remains mostly unexplored.Herein,we apply single-cell assay for transposaseaccessible chromatin sequencing to profile the genome-wide chromatin accessibility of 9,549 single cells from the m OB.Based on single-cell epigenetic signatures,m OB cells are classified into 21 clusters corresponding to 11 cell types.We identify distinct sets of putative regulatory elements specific to each cell cluster from which putative target genes and enriched potential functions are inferred.In addition,the transcription factor motifs enriched in each cell cluster are determined to indicate the developmental fate of each cell lineage.Our study provides a valuable epigenetic data set for the m OB at single-cell resolution,and the results can enhance our understanding of regulatory circuits and the therapeutic capacity of the OB at the single-cell level.展开更多
Civets are small mammals belonging to the family Viverridae.The masked palm civets(Paguma larvata)served as an intermediate host in the bat-to-human transmission of severe acute respiratory syndrome coronavirus(SARS-C...Civets are small mammals belonging to the family Viverridae.The masked palm civets(Paguma larvata)served as an intermediate host in the bat-to-human transmission of severe acute respiratory syndrome coronavirus(SARS-Co V)in 2003(Guan et al.,2003).Because of their unique role in the SARS outbreak,civets were suspected as a potential intermediate host of SARS-Co V-2.展开更多
基金Sponsored by the National Natural Science Foundation of China(Grant Nos.72061022 and 72171037).
文摘In this study,an optimization model of a single machine system integrating imperfect preventive maintenance planning and production scheduling based on game theory is proposed.The costs of the production department and the maintenance department are minimized,respectively.Two kinds of three-stage dynamic game models and a backward induction method are proposed to determine the preventive maintenance(PM)threshold.A lemma is presented to obtain the exact solution.A comprehensive numerical study is provided to illustrate the proposed maintenance model.The effectiveness is also validated by comparison with other two existed optimization models.
基金the National Natural Science Foundation of China(81972698).
文摘Gallbladder carcinoma (GBC) is a malignant tumor of the bil-iary system that is aggressive, difficult to detect early, and has a low surgical resection rate and poor prognosis. Ap-propriate in vitro growth models are expected to focus on the study of the biological behavior and assess treatment effects. Nonetheless, cancer initiation, progression, and in-vasion include spatiotemporal changes and changes in the cell microenvironment intracellular communication, and in-tracellular molecules, making the development of in vitro growth models very challenging. Recent advances in bioma-terial methods and tissue engineering, particularly advances in bioprinting procedures, have paved the way for advances in the creative phase of in vitro cancer research. To date, an increasing number of cultured models of gallbladder disease have emerged, such as two-dimensional (2D) GBC growth cell cultures, three-dimensional (3D) GBC growth cell cul-tures, xenograft models, and 3D bioprinting methods. These models can serve as stronger platforms, focusing on tumor growth initiation, the association with the microenvironment, angiogenesis, motility, aggression, and infiltration. Bioprint-ed growth models can also be used for high-throughput drug screening and validation, as well as translational opportuni-ties for individual cancer therapy. This study focused on the exploration, progress, and significance of the development of GBC cultural models. We present our views on the short-comings of existing models, investigate new innovations, and plan future improvements and application possibilities for cancer models.
基金the China Postdoctoral Science Foundation(2017M622795)the Science,Technology and Innovation Commission of Shenzhen Municipality(JCYJ20180507183628543)the Fundamental Research Funds for the Central Universities(2662018PY025 and 2662017PY105)。
基金supported by Shenzhen Sanming Engineering Project(SZSM202011012)Shenzhen Innovation Science and Technology Committee(JCYJ20180228175358223)National Natural Science Foundation of China(31670742)。
文摘Olfaction,the sense of smell,is a fundamental trait crucial to many species.The olfactory bulb(OB)plays pivotal roles in processing and transmitting odor information from the environment to the brain.The cellular heterogeneity of the mouse OB has been studied using single-cell RNA sequencing.However,the epigenetic landscape of the m OB remains mostly unexplored.Herein,we apply single-cell assay for transposaseaccessible chromatin sequencing to profile the genome-wide chromatin accessibility of 9,549 single cells from the m OB.Based on single-cell epigenetic signatures,m OB cells are classified into 21 clusters corresponding to 11 cell types.We identify distinct sets of putative regulatory elements specific to each cell cluster from which putative target genes and enriched potential functions are inferred.In addition,the transcription factor motifs enriched in each cell cluster are determined to indicate the developmental fate of each cell lineage.Our study provides a valuable epigenetic data set for the m OB at single-cell resolution,and the results can enhance our understanding of regulatory circuits and the therapeutic capacity of the OB at the single-cell level.
文摘Civets are small mammals belonging to the family Viverridae.The masked palm civets(Paguma larvata)served as an intermediate host in the bat-to-human transmission of severe acute respiratory syndrome coronavirus(SARS-Co V)in 2003(Guan et al.,2003).Because of their unique role in the SARS outbreak,civets were suspected as a potential intermediate host of SARS-Co V-2.
