[Objectives]To study the effects of Fruticosa potentilla L.on the expression of key enzymes and hormones related to glucose and lipid metabolism in T2DM rats.[Methods]The ethyl acetate fraction of 95%methanol extract ...[Objectives]To study the effects of Fruticosa potentilla L.on the expression of key enzymes and hormones related to glucose and lipid metabolism in T2DM rats.[Methods]The ethyl acetate fraction of 95%methanol extract of F.potentilla(MEE)was administered orally to the T2DM rats,and the level of glucose and expression of insulin,resistin,leptin,glucokinase(GK)and glucose-6-phosphatase(G-6-Pase)in the rats were measured and detected.[Results]In the T2DM rats administered with MEE,the postprandial blood glucose level decreased,the expression levels of resistin,leptin and G-6-Pase were down-regulated,and the expression levels of insulin and GK were up-regulated(P<0.05,P<0.01).[Conclusions]F.potentilla can reduce effectively postprandial blood glucose level,regulate the expression of a variety of enzymes and hormones related to glucose and lipid metabolism and recover partially the insulin level in T2DM rats to achieve resistance to T2DM.展开更多
In order to thoroughly investigate the diversity of glacier microorganisms, four DNA extraction methods with differem lysis pat- terns were tested and two screened methods （the Bosshard-Bano method and the Zhou metho...In order to thoroughly investigate the diversity of glacier microorganisms, four DNA extraction methods with differem lysis pat- terns were tested and two screened methods （the Bosshard-Bano method and the Zhou method） were optimized for the most ef- fective form of the filter membrane （cut vs. uncut）, the DNA extraction method, and the precipitation method. The two optimized methods were then compared with the commercial Mo-Bio DNA extraction kit, and the results showed that the kit was generally suitable for extraction of microorganism DNA fi＇om glacier surface snow. Procedurally, it was found that a modified Boss- hard-Bano method （i.e., cutting the filter membrane into pieces, using a specific lysis pattern [lysozyme （5 mg/mL）-protease K （ 1 mg/mL）-CTAB （ 1%）-SDS （ 1%）], performing the extraction only once by chloroform-isoamyl alcohol （24： 1）, and conducting DNA precipitation by pure ethanol） was also an effective and less expensive method for extraction of microorganism DNA from glacier surface snow.展开更多
基金Supported by National Natural Science Foundation of China(No.81460652)Key Research and Development and Achievements Transformation Program of Qinghai Province(No.2018-SF-144)Project Funded by Key Laboratory of Medicinal Animal and Plant Resources of Qinghai-Tibet Plateau in Qinghai Province(No.2017-z-y25)
文摘[Objectives]To study the effects of Fruticosa potentilla L.on the expression of key enzymes and hormones related to glucose and lipid metabolism in T2DM rats.[Methods]The ethyl acetate fraction of 95%methanol extract of F.potentilla(MEE)was administered orally to the T2DM rats,and the level of glucose and expression of insulin,resistin,leptin,glucokinase(GK)and glucose-6-phosphatase(G-6-Pase)in the rats were measured and detected.[Results]In the T2DM rats administered with MEE,the postprandial blood glucose level decreased,the expression levels of resistin,leptin and G-6-Pase were down-regulated,and the expression levels of insulin and GK were up-regulated(P<0.05,P<0.01).[Conclusions]F.potentilla can reduce effectively postprandial blood glucose level,regulate the expression of a variety of enzymes and hormones related to glucose and lipid metabolism and recover partially the insulin level in T2DM rats to achieve resistance to T2DM.
基金supported by the National Natural Science Foundation of China (Nos. 40825017, 40576001 and 31100369)
文摘In order to thoroughly investigate the diversity of glacier microorganisms, four DNA extraction methods with differem lysis pat- terns were tested and two screened methods （the Bosshard-Bano method and the Zhou method） were optimized for the most ef- fective form of the filter membrane （cut vs. uncut）, the DNA extraction method, and the precipitation method. The two optimized methods were then compared with the commercial Mo-Bio DNA extraction kit, and the results showed that the kit was generally suitable for extraction of microorganism DNA fi＇om glacier surface snow. Procedurally, it was found that a modified Boss- hard-Bano method （i.e., cutting the filter membrane into pieces, using a specific lysis pattern [lysozyme （5 mg/mL）-protease K （ 1 mg/mL）-CTAB （ 1%）-SDS （ 1%）], performing the extraction only once by chloroform-isoamyl alcohol （24： 1）, and conducting DNA precipitation by pure ethanol） was also an effective and less expensive method for extraction of microorganism DNA from glacier surface snow.
