基于微信平台互动的参与式教学法在西医院校针灸见习中的应用

为了探讨基于微信平台互动的参与式教学法在西医院校中医学针灸临床见习教学中的应用价值。将144名临床医学专业本科学生随机分为2组,试验组和对照组各72人。试验组采用基于微信平台互动的参与式教学法,对照组采用传统讲授式教学法,进行中医学针灸临床见习,见习结束后,采用教学满意度调查和操作考核的方法进行教学效果评估。结果表明,试验组教学满意度调查优于对照组(P<0.05),试验组针刺及艾灸的操作考核成绩高于对照组(P<0.05)。因此,基于微信平台互动的参与式教学法在西医院校中医学针灸临床见习中能取得较好的教学效果,值得推广应用。

COPD肺组织中MAL、IL-10、TNF-α的表达

目的观察COPD肺组织中MAL、IL-10、TNF-α的表达。方法收集2012-2015年在南华大学附属第一医院接受肺癌全肺切除术的患者病历,选择非肿瘤浸润的邻近肺组织。根据COPD诊断标准和患者资料,将标本分为肺功能正常组和COPD组的肺组织,每组5例。取SD大鼠16只,全部为雄性健康体,按照每组8只随机分为两组,一组为COPD模型组,另一组为正常组。采用HE染色法对人及实验大鼠的肺组织进行形态观测,IHC检测人及大鼠肺组织中MAL的表达及分布,ELISA检测大鼠肺组织中MAL、TNF-α、IL-10的蛋白水平。结果罹患COPD的人及大鼠肺组织中MAL蛋白表达水平低于正常组(P<0.05);与正常组相比,COPD大鼠肺组织中有大量炎症细胞浸润,MAL和IL-10表达明显减弱,TNF-α表达上调,差异均具统计学意义。结论MAL在COPD患者和大鼠肺组织中表达下调,IL-10在大鼠肺组织中表达下调,TNF-α在大鼠肺组织中表达上调,MAL与IL-10、TNF-α的表达水平显著相关。

刘鑫治疗肺结节临床经验

肺结节是通过影像学检查并结合临床表现、病史、相关实验室检查等进行诊断,中医学对于肺结节的相关记载很少,现代医家常常把肺结节归属“咳嗽”“肺积”等范畴来进行治疗。刘鑫教授结合多年临床经验,从患者的临床症状出发,结合四诊,辨证论治,个性化用药,现将刘鑫教授治疗肺结节的临床经验进行归纳分享。

柴朴汤含药血清对气道上皮细胞株中MAL、CADM1、TSLP、YKL-40表达的影响

目的探讨柴朴汤含药血清对气道上皮细胞(Airway epithelial cell,AEC)中T细胞成熟相关蛋白(MAL)、细胞黏附分子1(CADM1)、胸腺间质淋巴细胞生成素(TSLP)、甲壳质酶蛋白40(YKL-40)表达的影响。方法制备空白、肝郁型哮喘及柴朴汤含药血清,分别加入正常小鼠AEC中培养,分为正常组(A)、肝郁型哮喘组(B)和柴朴汤组(C)。采用酶联免疫吸附法(ELISA)检测TSLP、YKL-40在小鼠气道上皮细胞的上清液中的浓度,实时荧光定量PCR(qRT-PCR)法和蛋白免疫印迹法(WB)检测各组气道上皮细胞中MAL、CADM1、TSLP、YKL-40 mRNA及蛋白的表达情况。结果经肝郁型哮喘血清诱导后,气道上皮细胞中MAL、CADM1 mRNA及蛋白表达减少,TSLP、YKL-40 mRNA及蛋白表达增加,上清液中TSLP、YKL-40的浓度增加,与正常组相比有统计学意义(P<0.05);柴朴汤含药血清诱导后,气道上皮细胞中MAL mRNA和蛋白表达增加,TSLP、YKL-40 mRNA及蛋白表达减少,上清液中TSLP、YKL-40的浓度减少,与肝郁型哮喘组相比有统计学意义(P<0.05),CADM1 mRNA和蛋白表达无明显变化。结论①肝郁型哮喘血清可诱导AEC中CADM1、MAL表达降低,TSLP、YKL-40表达升高。②柴朴汤含药血清可上调肝郁型哮喘血清培养AEC中MAL的表达,下调TSLP、YKL-40表达。

Clinical observation on the time-effect relationship of moxibustion for primary dysmenorrhea due to stagnation and congelation of cold-damp

Objective:To observe the time-effect relationship of moxibustion for primary dysmenorrhea(PD)due to stagnation and congelation of cold-damp,thus explore the optimal choice of moxibustion duration,and provide evidence for achieving satisfactory efficacy in moxibustion treatment.Methods:A total of 90 patients with PD due to stagnatin and congelation of cold-damp were divided into three groups by the random number table method,with 30 cases in each group.All the patients in the three groups were given moxibustion treatment at Guanyuan(CV 4),20 min in group A,40 min in group B and 60 min in group C.The changes in the pain measurement score in the three groups were observed after treatment.Results:After treatment,there were significant differences in the clinical efficacy among the three groups(P<0.05);the clinical efficacy was better in group B and group C than that in group A(P<0.05),and that in group B was better than that in group C(P<0.05).Besides,the pain measurement score changed significantly after treatment in the three groups(all P<0.05),and the between-group differences were also statistically significant(P<0.05);the pain measurement scores in group B and group C were lower than that in group A(P<0.05),and that in group B was lower than that in group C(P<0.05).Conclusion:Given the same stimulating frequency and intervention time of moxibustion,40-minute duration demonstrates relatively better efficacy for PD due to stagnation and congelation of cold-damp.