LaminB1,a major component of the nuclear lamina,is a potent regulator of cellular proliferation and senescence and also known to be essential for neuronal migration and brain development.However,the expression pattern...LaminB1,a major component of the nuclear lamina,is a potent regulator of cellular proliferation and senescence and also known to be essential for neuronal migration and brain development.However,the expression patterns of LaminB1 in the rat cochleae are still not fully revealed.Utilizing immunofluorescence.Western blotting,and quantitative real-time PCR,we identified the distribution and expression of LaminB1 in the rat cochleae.Immunofluorescence staining indicated that LaminBl was mainly localized in the auditory hair cells(HCs),spiral ganglion cells(SGC),stria vascularis(STV,including spiral ligament),Reissner's membrane(RM),and limbus laminae spiralis(LLS).Western blotting analysis illustrated that the distribution of LaminBl in rat cochleae was characterized by tissue specificity.The LaminB1 protein was expressed more in SGC and basilar membrane(BM)than in STV.Meanwhile,the mRNA expression of LaminB1 displayed difference in cochlear tissues.These observations preliminarily revealed the expression patterns of LaminB1,providing a theoretical basis for further study on the role of LaminB1 in auditory function.展开更多
基金the National Natural Science Foundation of China(No.81771004,No.81500791,and No.81300827).
文摘LaminB1,a major component of the nuclear lamina,is a potent regulator of cellular proliferation and senescence and also known to be essential for neuronal migration and brain development.However,the expression patterns of LaminB1 in the rat cochleae are still not fully revealed.Utilizing immunofluorescence.Western blotting,and quantitative real-time PCR,we identified the distribution and expression of LaminB1 in the rat cochleae.Immunofluorescence staining indicated that LaminBl was mainly localized in the auditory hair cells(HCs),spiral ganglion cells(SGC),stria vascularis(STV,including spiral ligament),Reissner's membrane(RM),and limbus laminae spiralis(LLS).Western blotting analysis illustrated that the distribution of LaminBl in rat cochleae was characterized by tissue specificity.The LaminB1 protein was expressed more in SGC and basilar membrane(BM)than in STV.Meanwhile,the mRNA expression of LaminB1 displayed difference in cochlear tissues.These observations preliminarily revealed the expression patterns of LaminB1,providing a theoretical basis for further study on the role of LaminB1 in auditory function.
