Gelatin methacryloyl(GelMA)hydrogels is a widely used bioink because of its good biological properties and tunable physicochemical properties,which has been widely used in a variety of tissue engineering and tissue re...Gelatin methacryloyl(GelMA)hydrogels is a widely used bioink because of its good biological properties and tunable physicochemical properties,which has been widely used in a variety of tissue engineering and tissue regeneration.However,pure GelMA is limited by the weak mechanical strength and the lack of continuous osteogenic induction environment,which is difficult to meet the needs of bone repair.Moreover,GelMA hydrogels are unable to respond to complex stimuli and therefore are unable to adapt to physiological and pathological microenvironments.This review focused on the functionalization strategies of GelMA hydrogel based bioinks for bone regeneration.The synthesis process of GelMA hydrogel was described in details,and various functional methods to meet the requirements of bone regeneration,including mechanical strength,porosity,vascularization,osteogenic differentiation,and immunoregulation for patient specific repair,etc.In addition,the response strategies of smart GelMA-based bioinks to external physical stimulation and internal pathological microenvironment stimulation,as well as the functionalization strategies of GelMA hydrogel to achieve both disease treatment and bone regeneration in the presence of various common diseases(such as inflammation,infection,tumor)are also briefly reviewed.Finally,we emphasized the current challenges and possible exploration directions of GelMA-based bioinks for bone regeneration.展开更多
基金The National Key R&D Program of China(2023YFC2414100)National Natural Science Foundation of China(32271379,82072396 and 81700954)+4 种基金Science and Technology Commission of Shanghai Municipality(21490711700)the Interdisciplinary Program of Shanghai Jiao Tong University(YG2021ZD12)Cross Disciplinary Research Fund of Shanghai Ninth People’s Hospital,Shanghai Jiao Tong University School of Medicine(JYJC202219)Shanghai’s Top Priority Research Center(2022ZZ01017)CAMS Innovation Fund for Medical Sciences(CIFMS,2019-I2M-5-037)supported this work.
文摘Gelatin methacryloyl(GelMA)hydrogels is a widely used bioink because of its good biological properties and tunable physicochemical properties,which has been widely used in a variety of tissue engineering and tissue regeneration.However,pure GelMA is limited by the weak mechanical strength and the lack of continuous osteogenic induction environment,which is difficult to meet the needs of bone repair.Moreover,GelMA hydrogels are unable to respond to complex stimuli and therefore are unable to adapt to physiological and pathological microenvironments.This review focused on the functionalization strategies of GelMA hydrogel based bioinks for bone regeneration.The synthesis process of GelMA hydrogel was described in details,and various functional methods to meet the requirements of bone regeneration,including mechanical strength,porosity,vascularization,osteogenic differentiation,and immunoregulation for patient specific repair,etc.In addition,the response strategies of smart GelMA-based bioinks to external physical stimulation and internal pathological microenvironment stimulation,as well as the functionalization strategies of GelMA hydrogel to achieve both disease treatment and bone regeneration in the presence of various common diseases(such as inflammation,infection,tumor)are also briefly reviewed.Finally,we emphasized the current challenges and possible exploration directions of GelMA-based bioinks for bone regeneration.
