A method is proposed to fuse the velocity data of the global navigation satellite system(GNSS) and leveling height via combined adjustment with constraints. First, stable GNSS-leveling points are uniformly selected, a...A method is proposed to fuse the velocity data of the global navigation satellite system(GNSS) and leveling height via combined adjustment with constraints. First, stable GNSS-leveling points are uniformly selected, and the constraints of the geodetic height change velocity and normal height change velocity are given. Then, the GNSS vertical velocities and leveling height difference are used as observations of combined adjustment, and robust least-squares estimation are used to estimate the velocities of the unknown points. Finally, a vertical movement model is established with the GNSS vertical velocities and leveling vertical velocities obtained via combined adjustment. Data from the second-order leveling network and GNSS control points in Shandong Province are taken as test data, and eight calculation schemes are used for discussion. One of the schemes, the bifactor robust combined adjustment method based on variance component estimation with two kinds of vertical velocity constraints achieves the optimal results. The method applied in the scheme can be recommended for data fusion of GNSS and leveling, further improving the reliability of vertical crustal movement in Shandong Province.展开更多
Cyclophilin A (CypA) is a peptidyl-prolyl cis/trans isomerase that interacts with the matrix protein (M1) of influenza A virus (IAV) and restricts virus replication by regulating the ubiquitin–proteasome-mediated deg...Cyclophilin A (CypA) is a peptidyl-prolyl cis/trans isomerase that interacts with the matrix protein (M1) of influenza A virus (IAV) and restricts virus replication by regulating the ubiquitin–proteasome-mediated degradation of M1. However,the mechanism by which CypA regulates M1 ubiquitination remains unknown. In this study, we reported that E3 ubiquitin ligase AIP4 promoted K48-linked ubiquitination of M1 at K102 and K104, and accelerated ubiquitin–proteasome-mediated degradation of M1. The recombinant IAV with mutant M1 (K102 R/K104 R) could not be rescued, suggesting that the ubiquitination of M1 at K102/K104 was essential for IAV replication. Furthermore, CypA inhibited AIP4-mediated M1 ubiquitination by impairing the interaction between AIP4 and M1. More importantly, both the mutations of M1 (K102 R/K104 R) and CypA inhibited the nuclear export of M1, indicating that CypA regulates the cellular localization of M1 via inhibition of AIP4-mediated M1 ubiquitination at K102 and K104, which results in the reduced replication of IAV.Collectively, our findings reveal a novel ubiquitination-based mechanism by which CypA regulates the replication of IAV.展开更多
Dear Editor,The ongoing SARS-CoV-2 pandemic has lasted for 2 years,causing a severe global impact on social development.With widespread vaccinations,the number of new infections has dropped significantly,but with the ...Dear Editor,The ongoing SARS-CoV-2 pandemic has lasted for 2 years,causing a severe global impact on social development.With widespread vaccinations,the number of new infections has dropped significantly,but with the emergence of diverse novel variants of concern(VOCs)and variants of interest(VOIs),there has been a second or third epidemic wave in many countries.展开更多
基金supported by the National Natural Science Foundation of China(41774004,41904040)the Technological Innovation of SHASG(SCK2020-11).
文摘A method is proposed to fuse the velocity data of the global navigation satellite system(GNSS) and leveling height via combined adjustment with constraints. First, stable GNSS-leveling points are uniformly selected, and the constraints of the geodetic height change velocity and normal height change velocity are given. Then, the GNSS vertical velocities and leveling height difference are used as observations of combined adjustment, and robust least-squares estimation are used to estimate the velocities of the unknown points. Finally, a vertical movement model is established with the GNSS vertical velocities and leveling vertical velocities obtained via combined adjustment. Data from the second-order leveling network and GNSS control points in Shandong Province are taken as test data, and eight calculation schemes are used for discussion. One of the schemes, the bifactor robust combined adjustment method based on variance component estimation with two kinds of vertical velocity constraints achieves the optimal results. The method applied in the scheme can be recommended for data fusion of GNSS and leveling, further improving the reliability of vertical crustal movement in Shandong Province.
基金supported by grants from the National Natural Science Foundation of China (31630079,31672531,31572526,and 31802164)the National Key R&D Program of China (2016YFD0500206,2015BAD11B02)+3 种基金the Strategic Priority Research Program of Chinese Academy of Sciences (XDB29010000)the National Science and Technology Major Project (2018ZX10101004)the Emergency Technology Research Issue on Prevention and Control for Human Infection with A (H7N9) Avian Influenza Virus (10600100000015001206)Wenjun Liu is the principal investigator of the Innovative Research Group of National Natural Science Foundation of China (Grant No.81621091)
文摘Cyclophilin A (CypA) is a peptidyl-prolyl cis/trans isomerase that interacts with the matrix protein (M1) of influenza A virus (IAV) and restricts virus replication by regulating the ubiquitin–proteasome-mediated degradation of M1. However,the mechanism by which CypA regulates M1 ubiquitination remains unknown. In this study, we reported that E3 ubiquitin ligase AIP4 promoted K48-linked ubiquitination of M1 at K102 and K104, and accelerated ubiquitin–proteasome-mediated degradation of M1. The recombinant IAV with mutant M1 (K102 R/K104 R) could not be rescued, suggesting that the ubiquitination of M1 at K102/K104 was essential for IAV replication. Furthermore, CypA inhibited AIP4-mediated M1 ubiquitination by impairing the interaction between AIP4 and M1. More importantly, both the mutations of M1 (K102 R/K104 R) and CypA inhibited the nuclear export of M1, indicating that CypA regulates the cellular localization of M1 via inhibition of AIP4-mediated M1 ubiquitination at K102 and K104, which results in the reduced replication of IAV.Collectively, our findings reveal a novel ubiquitination-based mechanism by which CypA regulates the replication of IAV.
基金This study was supported by National Key Technologies Research and Development Program grants 2018YFC1200500 and 2018YFC1200600 to L.Y.and 2021YFC2300900 to Y.B.,Strategic Priority Research Program of Chinese Academy of Sciences XDB29010000 to W.L.and XDB29010102 to Y.B.,National Natural Science Foundation of China(NSFC)(32041010)NSFC Outstanding Young Scholars(31822055)Youth Innovation Promotion Association of CAS(2017122)to Y.B.
文摘Dear Editor,The ongoing SARS-CoV-2 pandemic has lasted for 2 years,causing a severe global impact on social development.With widespread vaccinations,the number of new infections has dropped significantly,but with the emergence of diverse novel variants of concern(VOCs)and variants of interest(VOIs),there has been a second or third epidemic wave in many countries.
