Metastasis-related genes in hepatocellular carcinoma cell-lines are clustered on chromosome territories predicted by transcriptome and proteome

Recently,we have analyzed expressional transcriptome and proteome for a number of cancer cell lines,including 8hepatocellular carcinoma(HCC)ones[1,2].These 8 HCC cell lines consist of 6 metastatic(all with mutant p53)and 2non-metastatic(with a mutant and a lost p53)ones,being all HBV traceable for relatively close genetic-backgrounds.We have analyzed the related dataset and obtained transcriptome(16353 genes)and proteome(7861 proteins)of these 8HCC cell lines,and explored a group of up-regulated

OGP: A Repository of Experimentally Characterized O-glycoproteins to Facilitate Studies on O-glycosylation

Numerous studies on cancers, biopharmaceuticals, and clinical trials have necessitated comprehensive and precise analysis of protein O-glycosylation. However, the lack of updated and convenient databases deters the storage of and reference to emerging O-glycoprotein data. To resolve this issue, an O-glycoprotein repository named OGP was established in this work.It was constructed with a collection of O-glycoprotein data from different sources. OGP contains 9354 O-glycosylation sites and 11,633 site-specific O-glycans mapping to 2133 O-glycoproteins, and it is the largest O-glycoprotein repository thus far.Based on the recorded O-glycosylation sites, an O-glycosylation site prediction tool was developed. Moreover, an OGP-based website is already available(http://www.oglyp.org/). The website comprises four specially designed and user-friendly modules:statistical analysis, database search, site prediction, and data submission. The first version of OGP repository and the website allow users to obtain various O-glycoprotein-related information, such as protein accession Nos., O-glycosylation sites,O-glycopeptide sequences, site-specific O-glycan structures, experimental methods, and potential O-glycosylation sites.O-glycosylation data mining can be performed efficiently on this website, which will greatly facilitate related studies. In addition, the database is accessible from OGP website(http://www.oglyp.org/download.php).

SIRT5 is important for bacterial infection by regulating insulin secretion and glucose homeostasis

Dear Editor,Recent studies indicate that cellular metabolism plays a key role in supporting immune cell development,maintenance and function(Norata et al.,2015).For example,sensing of microbial ligands by macrophages triggers increased glucose metabolism,which delivers energy to support antimicrobial inflammation and the production of cytokines.This effect further enhances the generation of mitochondrial reactive oxygen species(ROS)to fight infections.

Multimodal Studies in Hepatitis B Virus Associated Hepatocellular Carcinoma

Hepatocellular carcinoma(HCC)is a highly aggressive cancer that ranks the second leading cause of cancer related death.Hepatitis B virus(HBV)infection is the most prevalent etiological factor,especially in eastern world.However,the underlying mechanism of HBV infection-initialed carcinogenic progression remains largely unknown,making it difficult to improve therapeutic strategies for HBV-associated HCC(HBV+HCC).The virus drives multi-omics changes in human liver cells,leading to genomic in stability,epigenomic modifications,and proteomic a lterations.HBV infection also orchestrates the immunosuppressive microenv ironment in HBV+HCC.This review summarized recent research progress with the multimodal methods covering genome,transcriptome,epigenome,and proteome introduced in the mechanistic studies for HBV+HCC.