Uncontrolled hemorrhage resulting from traumas causes severe health risks.There is an urgent need for expeditious hemostatic materials to treat bleeding incidents.Here,we developed a natural protein-based hemostatic s...Uncontrolled hemorrhage resulting from traumas causes severe health risks.There is an urgent need for expeditious hemostatic materials to treat bleeding incidents.Here,we developed a natural protein-based hemostatic sponge extracted from nonmulberry cocoons that exhibited rapid coagulation and effective absorption.We first built a degumming and dissolution system suitable for the Dictyoploca japonica cocoons to obtain regenerated silk fibroin(DSF).The DSF was then combined with carboxymethyl chitosan(CMCS)by glutaraldehyde(GA)crosslinking to ensure the structural stability of sponges.The resulting DSF–CMCS–GA exhibited remarkable hemostatic properties,displaying the highest absorption rate.It also demonstrated comparable efficacy to commercial hemostatic sponges.The blood-clotting index and hemolysis test showed that the prepared sponge possessed hemostatic activity and good hemocompatibility.Compared with mulberry silk fibroin hemostatic sponges(SF–CMCS–GA),DSF–CMCS–GA showed slightly better effects,making them a potential alternative to mulberry silk.In conclusion,our study introduces the use of Dictyoploca japonica silk fibroin for hemostasis,highlighting the exploitation of wild silkworm resources and providing an excellent silk fibroin-based hemostatic sealant for acute accident wounds and biomedical applications involving massive hemorrhage.展开更多
基金the National Natural Science Foundation of China(Grant Nos.51672250,32301177,and 51902289)the Key Research&Development Program of Zhejiang Province(Grant Nos.2021C01180 and 2019C04020)the Scientific Research Foundation of Zhejiang Sci-Tech University(Grant No.22212238-Y).
文摘Uncontrolled hemorrhage resulting from traumas causes severe health risks.There is an urgent need for expeditious hemostatic materials to treat bleeding incidents.Here,we developed a natural protein-based hemostatic sponge extracted from nonmulberry cocoons that exhibited rapid coagulation and effective absorption.We first built a degumming and dissolution system suitable for the Dictyoploca japonica cocoons to obtain regenerated silk fibroin(DSF).The DSF was then combined with carboxymethyl chitosan(CMCS)by glutaraldehyde(GA)crosslinking to ensure the structural stability of sponges.The resulting DSF–CMCS–GA exhibited remarkable hemostatic properties,displaying the highest absorption rate.It also demonstrated comparable efficacy to commercial hemostatic sponges.The blood-clotting index and hemolysis test showed that the prepared sponge possessed hemostatic activity and good hemocompatibility.Compared with mulberry silk fibroin hemostatic sponges(SF–CMCS–GA),DSF–CMCS–GA showed slightly better effects,making them a potential alternative to mulberry silk.In conclusion,our study introduces the use of Dictyoploca japonica silk fibroin for hemostasis,highlighting the exploitation of wild silkworm resources and providing an excellent silk fibroin-based hemostatic sealant for acute accident wounds and biomedical applications involving massive hemorrhage.
