The story of phosphorothioate modifications of DNA begins over 50 years ago and has unfolded like Frost's walk in the woods[1].In 1970,Fritz Eckstein synthesized oligonucleotides in which a non-bridging oxygen in ...The story of phosphorothioate modifications of DNA begins over 50 years ago and has unfolded like Frost's walk in the woods[1].In 1970,Fritz Eckstein synthesized oligonucleotides in which a non-bridging oxygen in the phosphodiester backbone was replaced by sulfur–the first phosphorothioate-modified nucleic acid[2].Twenty years passed until Zixin Deng and colleagues discovered a DNA degradation phenotype in bacteria possessing a 5-gene dnd cluster,with electrophoretic degradation caused by incorporation of sulfur into bacterial genomes[3].These roads of chemistry and biology met again another 20 years later with the discovery by Wang et al.that Dnd proteins insert sulfur as a phosphorothioate in the genomes of diverse bacteria[4].This walk in the woods now picked up speed.展开更多
基金the National Science Foundation of the USA(CHE-1709364)the National Research Foundation of Singapore in the Singapore-MIT Alliance for Research and Technology Antimicrobial Resistance IRG.
文摘The story of phosphorothioate modifications of DNA begins over 50 years ago and has unfolded like Frost's walk in the woods[1].In 1970,Fritz Eckstein synthesized oligonucleotides in which a non-bridging oxygen in the phosphodiester backbone was replaced by sulfur–the first phosphorothioate-modified nucleic acid[2].Twenty years passed until Zixin Deng and colleagues discovered a DNA degradation phenotype in bacteria possessing a 5-gene dnd cluster,with electrophoretic degradation caused by incorporation of sulfur into bacterial genomes[3].These roads of chemistry and biology met again another 20 years later with the discovery by Wang et al.that Dnd proteins insert sulfur as a phosphorothioate in the genomes of diverse bacteria[4].This walk in the woods now picked up speed.
