This study presents a rapid and versatile low-cost sample-to-answer system for SARS-CoV-2 diagnostics.The system integrates the extraction and purification of nucleic acids,followed by amplification via either reverse...This study presents a rapid and versatile low-cost sample-to-answer system for SARS-CoV-2 diagnostics.The system integrates the extraction and purification of nucleic acids,followed by amplification via either reverse transcriptionquantitative polymerase chain reaction(RT–qPCR)or reverse transcription loop-mediated isothermal amplification(RT–LAMP).By meeting diverse diagnostic and reagent needs,the platform yields testing results that closely align with those of commercial RT-LAMP and RT‒qPCR systems.Notable advantages of our system include its speed and costeffectiveness.The assay is completed within 28 min,including sample loading(5 min),ribonucleic acid(RNA)extraction(3 min),and RT-LAMP(20 min).The cost of each assay is≈$9.5,and this pricing is competitive against that of Food and Drug Administration(FDA)-approved commercial alternatives.Although some RNA loss during on-chip extraction is observed,the platform maintains a potential limit of detection lower than 297 copies.Portability makes the system particularly useful in environments where centralized laboratories are either unavailable or inconveniently located.Another key feature is the platform’s versatility,allowing users to choose between RT‒qPCR or RT‒LAMP tests based on specific requirements.展开更多
基金support of Grant No.52150710541 from the National Natural Science Foundation of China(NSFC),P.R.of ChinaGrant No.2018YFE0109000 from the Ministry of Science and Technology of the P.R.of China+2 种基金Petra Vopařilováand other members of Mendel University would like to acknowledge financial support from the Internal Grant Agency of the Faculty of AgriSciences,Mendel University in Brno(No.AF-IGA2023-IP-030)Haoqing Zhang would like to acknowledge the financial support of Grant No.2023-JC-QN-0130 from the Natural Science Basic Research Program of Shaanxi Province,P.R.ChinaGrant No.2023M732815 from the P.R.China Postdoctoral Science Foundation.
文摘This study presents a rapid and versatile low-cost sample-to-answer system for SARS-CoV-2 diagnostics.The system integrates the extraction and purification of nucleic acids,followed by amplification via either reverse transcriptionquantitative polymerase chain reaction(RT–qPCR)or reverse transcription loop-mediated isothermal amplification(RT–LAMP).By meeting diverse diagnostic and reagent needs,the platform yields testing results that closely align with those of commercial RT-LAMP and RT‒qPCR systems.Notable advantages of our system include its speed and costeffectiveness.The assay is completed within 28 min,including sample loading(5 min),ribonucleic acid(RNA)extraction(3 min),and RT-LAMP(20 min).The cost of each assay is≈$9.5,and this pricing is competitive against that of Food and Drug Administration(FDA)-approved commercial alternatives.Although some RNA loss during on-chip extraction is observed,the platform maintains a potential limit of detection lower than 297 copies.Portability makes the system particularly useful in environments where centralized laboratories are either unavailable or inconveniently located.Another key feature is the platform’s versatility,allowing users to choose between RT‒qPCR or RT‒LAMP tests based on specific requirements.
