Objective:To investigate the inhibited effect of epigallocatechin-3-gallate(EGCG)on the expression of NPM1 in IMS-M2 cells harboring the NPMl mutations.Methods:Cell proliferation assay was performed to test the effect...Objective:To investigate the inhibited effect of epigallocatechin-3-gallate(EGCG)on the expression of NPM1 in IMS-M2 cells harboring the NPMl mutations.Methods:Cell proliferation assay was performed to test the effects of EGCG on eell growth of IMS-M2 cells harboring the NPM1 mutations.Western blot analysis were performed to test the protein expression of NPM1,AKT,those associated with apoplosis.Results:EGCG can down-regulate the expression of NPM1 in IMS-M2 cells harboring the NPM1mutations.Moreover,EGCG also suppressed the cell proliferation and induced apoptosis in IMSM2 cells.Conclusions:The results suggested that EGCG could be considered as a reagent for treatment of AML patients with NPM1 mutations.展开更多
基金Supported by the Japan Foundation for Promotion of International Medical Research Co-operation(JF-PIMRC)
文摘Objective:To investigate the inhibited effect of epigallocatechin-3-gallate(EGCG)on the expression of NPM1 in IMS-M2 cells harboring the NPMl mutations.Methods:Cell proliferation assay was performed to test the effects of EGCG on eell growth of IMS-M2 cells harboring the NPM1 mutations.Western blot analysis were performed to test the protein expression of NPM1,AKT,those associated with apoplosis.Results:EGCG can down-regulate the expression of NPM1 in IMS-M2 cells harboring the NPM1mutations.Moreover,EGCG also suppressed the cell proliferation and induced apoptosis in IMSM2 cells.Conclusions:The results suggested that EGCG could be considered as a reagent for treatment of AML patients with NPM1 mutations.
