In this paper,we present an approach called the free lens modulation(FLM)method to generate high-perfection3D generalized perfect optical vortices(GPOVs)with topological charges of 1–80.In addition,2D and 3D GPOVs we...In this paper,we present an approach called the free lens modulation(FLM)method to generate high-perfection3D generalized perfect optical vortices(GPOVs)with topological charges of 1–80.In addition,2D and 3D GPOVs were produced by altering the parameters of the freely shaped lenses.To verify the quality of the GPOVs produced with the FLM method,we conducted optical trapping experiments and realized linear control of the rotation rate of the trapped particle.Due to the great advantages of high perfection and high power usage in generating arbitrarily shaped GPOVs,the FLM method is expected to be applied in optical manipulation,optical communications,and other fields.展开更多
Super-resolution structured illumination microscopy(SR-SIM)is an outstanding method for visualizing the subcellular dynamics in living cells.To date,by using elaborately designed systems and algorithms,SR-SIM can achi...Super-resolution structured illumination microscopy(SR-SIM)is an outstanding method for visualizing the subcellular dynamics in living cells.To date,by using elaborately designed systems and algorithms,SR-SIM can achieve rapid,optically sectioned,SR observation with hundreds to thousands of time points.However,real-time observation is still out of reach for most SIM setups as conventional algorithms for image reconstruction involve a heavy computing burden.To address this limitation,an accelerated reconstruction algorithm was developed by implementing a simplified workflow for SR-SIM,termed joint space and frequency reconstruction.This algorithm results in an 80-fold improvement in reconstruction speed relative to the widely used Wiener-SIM.Critically,the increased processing speed does not come at the expense of spatial resolution or sectioning capability,as demonstrated by live imaging of microtubule dynamics and mitochondrial tubulation.展开更多
基金National Key Research and Development Program of China(2022YFF0712500)National Natural Science Foundation of China(61905189,62135003,62205267)+3 种基金Innovation Capability Support Program of Shaanxi(2021TD-57)Natural Science Basic Research Program of Shaanxi(2020JQ-072,2022JZ-34)Fundamental Research Funds for the Central Universities(xxj022019017)National Institutes of Health(GM144414)。
文摘In this paper,we present an approach called the free lens modulation(FLM)method to generate high-perfection3D generalized perfect optical vortices(GPOVs)with topological charges of 1–80.In addition,2D and 3D GPOVs were produced by altering the parameters of the freely shaped lenses.To verify the quality of the GPOVs produced with the FLM method,we conducted optical trapping experiments and realized linear control of the rotation rate of the trapped particle.Due to the great advantages of high perfection and high power usage in generating arbitrarily shaped GPOVs,the FLM method is expected to be applied in optical manipulation,optical communications,and other fields.
基金supported by the National Natural Science Foundation of China (NSFC) (Nos. 62005208, 62135003, and 61905189)Innovation Capability Support Program of Shaanxi (No. 2021TD-57)+1 种基金China Postdoctoral Science Foundation (Nos. 2020M673365 and 2019M663656)National Institutes of Health Grant GM100156 to PRB
文摘Super-resolution structured illumination microscopy(SR-SIM)is an outstanding method for visualizing the subcellular dynamics in living cells.To date,by using elaborately designed systems and algorithms,SR-SIM can achieve rapid,optically sectioned,SR observation with hundreds to thousands of time points.However,real-time observation is still out of reach for most SIM setups as conventional algorithms for image reconstruction involve a heavy computing burden.To address this limitation,an accelerated reconstruction algorithm was developed by implementing a simplified workflow for SR-SIM,termed joint space and frequency reconstruction.This algorithm results in an 80-fold improvement in reconstruction speed relative to the widely used Wiener-SIM.Critically,the increased processing speed does not come at the expense of spatial resolution or sectioning capability,as demonstrated by live imaging of microtubule dynamics and mitochondrial tubulation.
