Ultrawideband electromagnetic pulses with high amplitude and short duration are reported to affect several aspects of cell physiology. They are usually delivered to the living material through electrodes in small dedi...Ultrawideband electromagnetic pulses with high amplitude and short duration are reported to affect several aspects of cell physiology. They are usually delivered to the living material through electrodes in small dedicated chambers. Here we showed, using a totally different experimental setup, that radiated EM pulses illuminating the living material through a specialized antenna (without any direct contact) are able to trigger a rapid release of ATP in cultured murine cells that was concomitant with a drop of intracellular AEC. Despite this rapid and strong response, we found that cell viability and clonogenicity were only slightly affected by the EMF exposure.展开更多
The cultivation of seed mixtures for local pastures is a traditional mixed cropping technique of cereals and legumes for producing,at a low production cost,a balanced animal feed in energy and protein in livestock sys...The cultivation of seed mixtures for local pastures is a traditional mixed cropping technique of cereals and legumes for producing,at a low production cost,a balanced animal feed in energy and protein in livestock systems.By considerably improving the autonomy and safety of agricultural systems,as well as reducing their impact on the environment,it is a type of crop that responds favorably to both the evolution of the European regulations on the use of phytosanitary products and the expectations of consumers who wish to increase their consumption of organic products.However,farmers find it difficult to adopt it because cereals and legumes do not ripen synchronously and the harvested seeds are heterogeneous,making it more difficult to assess their nutritional value.Many efforts therefore remain to be made to acquire and aggregate technical and economical references to evaluate to what extent the cultivation of seed mixtures could positively contribute to securing and reducing the costs of herd feeding.The work presented in this paper proposes new Artificial Intelligence techniques that could be transferred to an online or smartphone application to automatically estimate the nutritional value of harvested seed mixes to help farmers better manage the yield and thus engage them to promote and contribute to a better knowledge of this type of cultivation.For this purpose,an original open image dataset has been built containing 4,749 images of seed mixes,covering 11 seed varieties,with which 2 types of recent deep learning models have been trained.The results highlight the potential of this method and show that the best-performing model is a recent state-of-the-art vision transformer pre-trained with self-supervision(Bidirectional Encoder representation from Image Transformer).It allows an estimation of the nutritional value of seed mixtures with a coefficient of determination R^(2)score of 0.91,which demonstrates the interest of this type of approach,for its possible use on a large scale.展开更多
文摘Ultrawideband electromagnetic pulses with high amplitude and short duration are reported to affect several aspects of cell physiology. They are usually delivered to the living material through electrodes in small dedicated chambers. Here we showed, using a totally different experimental setup, that radiated EM pulses illuminating the living material through a specialized antenna (without any direct contact) are able to trigger a rapid release of ATP in cultured murine cells that was concomitant with a drop of intracellular AEC. Despite this rapid and strong response, we found that cell viability and clonogenicity were only slightly affected by the EMF exposure.
基金This research work was mainly undertaken within the framework of the Carpeso project(AAPIP 2019 n°19AIP5902),supported by the CASDAR program(The Special Affection Account for Agricultural and Rural Development)funded by the French National Research Agency(ANR)through the grant PI@ntAgroEco 22-PEAE0009.
文摘The cultivation of seed mixtures for local pastures is a traditional mixed cropping technique of cereals and legumes for producing,at a low production cost,a balanced animal feed in energy and protein in livestock systems.By considerably improving the autonomy and safety of agricultural systems,as well as reducing their impact on the environment,it is a type of crop that responds favorably to both the evolution of the European regulations on the use of phytosanitary products and the expectations of consumers who wish to increase their consumption of organic products.However,farmers find it difficult to adopt it because cereals and legumes do not ripen synchronously and the harvested seeds are heterogeneous,making it more difficult to assess their nutritional value.Many efforts therefore remain to be made to acquire and aggregate technical and economical references to evaluate to what extent the cultivation of seed mixtures could positively contribute to securing and reducing the costs of herd feeding.The work presented in this paper proposes new Artificial Intelligence techniques that could be transferred to an online or smartphone application to automatically estimate the nutritional value of harvested seed mixes to help farmers better manage the yield and thus engage them to promote and contribute to a better knowledge of this type of cultivation.For this purpose,an original open image dataset has been built containing 4,749 images of seed mixes,covering 11 seed varieties,with which 2 types of recent deep learning models have been trained.The results highlight the potential of this method and show that the best-performing model is a recent state-of-the-art vision transformer pre-trained with self-supervision(Bidirectional Encoder representation from Image Transformer).It allows an estimation of the nutritional value of seed mixtures with a coefficient of determination R^(2)score of 0.91,which demonstrates the interest of this type of approach,for its possible use on a large scale.
