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SIMPLE METHOD OF PUNCHING AND RE-LOCATING TISSUES FOR MANUAL CONSTRUCTION OF TISSUE MICROARRAY
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作者 郑林 张帆 +3 位作者 王莉 颜召文 姜叙诚 pierre-tienne bouchet 《Journal of Shanghai Second Medical University(Foreign Language Edition)》 2007年第1期58-60,F0003,共4页
A series of human tissue samples and cultured cell lines were formalin-fixed and paraffin-embedded. Specimen cylinder (1.2 -1.8ram) were punched by a modified bone marrow biopsy needle and arrayed on a recipient par... A series of human tissue samples and cultured cell lines were formalin-fixed and paraffin-embedded. Specimen cylinder (1.2 -1.8ram) were punched by a modified bone marrow biopsy needle and arrayed on a recipient paraffin block. Microscopic analysis on the sections from this tissue microarray ( TMA ) block demonstrated that the spots of tissues and cells were well preserved, and the cultured cell samples were successfully embedded from 5 × 104 to 2 × 105 in number. These TMA sections were also suitable for immunohistochemistry and RNA in situ hybridization. 展开更多
关键词 tissue microarray IMMUNOHISTOCHEMISTRY in situ hybridization
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