December 9,2022 is the 90th birthday of Tong Zhang,a mathematician in Institute of Mathematics,Chinese Academy of Sciences where he was always working on the Riemann problem for gas dynamics in his mathematical life.T...December 9,2022 is the 90th birthday of Tong Zhang,a mathematician in Institute of Mathematics,Chinese Academy of Sciences where he was always working on the Riemann problem for gas dynamics in his mathematical life.To celebrate his 90th birthday and great contributions to this specifc feld,we organize this focused issue in the journal Communications on Applied Mathematics and Computation,since the Riemann problem has been proven to be a building block in all felds of theory,numerics and applications of hyperbolic conservation laws.展开更多
Background:Osteoporosis is a chronic bone disease characterized by bone loss and decreased bone strength.However,current anti-resorptive drugs carry a risk of various complications.The deep learning-based efficacy pre...Background:Osteoporosis is a chronic bone disease characterized by bone loss and decreased bone strength.However,current anti-resorptive drugs carry a risk of various complications.The deep learning-based efficacy prediction system(DLEPS)is a forecasting tool that can effectively compete in drug screening and prediction based on gene expression changes.This study aimed to explore the protective effect and potential mechanisms of cinobufotalin(CB),a traditional Chinese medicine(TCM),on bone loss.Methods:DLEPS was employed for screening anti-osteoporotic agents according to gene profile changes in primary osteoporosis.Micro-CT,histological and morphological analysis were applied for the bone protective detection of CB,and the osteogenic differentiation/function in human bone marrow mesenchymal stem cells(hBMMSCs)were also investigated.The underlying mechanism was verified using qRT-PCR,Western blot(WB),immunofluorescence(IF),etc.Results:A safe concentration(0.25mg/kg in vivo,0.05μM in vitro)of CB could effectively preserve bone mass in estrogen deficiency-induced bone loss and promote osteogenic differentiation/function of hBMMSCs.Both BMPs/SMAD and Wnt/β-catenin signaling pathways participated in CB-induced osteogenic differentiation,further regulating the expression of osteogenesis-associated factors,and ultimately promoting osteogenesis.Conclusion:Our study demonstrated that CB could significantly reverse estrogen deficiency-induced bone loss,further promoting osteogenic differentiation/function of hBMMSCs,with BMPs/SMAD and Wnt/β-catenin signaling pathways involved.展开更多
Astrocytes are the most abundant glial cells in the central nervous system;they participate in crucial biological processes,maintain brain structure,and regulate nervous system function.Exosomes are cell-derived extra...Astrocytes are the most abundant glial cells in the central nervous system;they participate in crucial biological processes,maintain brain structure,and regulate nervous system function.Exosomes are cell-derived extracellular vesicles containing various bioactive molecules including proteins,peptides,nucleotides,and lipids secreted from their cellular sources.Increasing evidence shows that exosomes participate in a communication network in the nervous system,in which astrocyte-derived exosomes play important roles.In this review,we have summarized the effects of exosomes targeting astrocytes and the astrocyte-derived exosomes targeting other cell types in the central nervous system.We also discuss the potential research directions of the exosome-based communication network in the nervous system.The exosome-based intercellular communication focused on astrocytes is of great significance to the biological and/or pathological processes in different conditions in the brain.New strategies may be developed for the diagnosis and treatment of neurological disorders by focusing on astrocytes as the central cells and utilizing exosomes as communication mediators.展开更多
The necrotrophic fungus, Sclerotinia sclerotiorum, employs an array of cell wall-degrading enzymes(CWDEs), including cellulase, to dismantle host cell walls. However, the molecular mechanisms through which S. scleroti...The necrotrophic fungus, Sclerotinia sclerotiorum, employs an array of cell wall-degrading enzymes(CWDEs), including cellulase, to dismantle host cell walls. However, the molecular mechanisms through which S. sclerotiorum degrades cellulose remain elusive. Here, we unveil a novel secretory cellobiohydrolase, SsdchA, characterized by a signal peptide and a Glyco_hydro_7(GH7) domain. SsdchA exhibits a robust expression of during early infection stages. Interestingly, colony morphology and growth rates remain unaffected across the wild-type, SsdchA deletion strains and SsdchA overexpression strains on potato dextrose agar(PDA) medium. Nevertheless, the pathogenicity and cellobiohydrolase activity decreased in the SsdchA deletion strains, but enhanced in the SsdchA overexpression strains. Moreover,the heterologous expression of SsdchA in Arabidopsis thaliana leads to reduced cellulose content and heightened susceptibility to S. sclerotiorum. Collectively, our data underscore the pivotal role of the novel cellobiohydrolase SsdchA in the pathogenicity of S. sclerotiorum.展开更多
Liver transplantation(LT)is the standard therapy for individuals afflicted with end-stage liver disease.Despite notable advancements in LT technology,the incidence of early allograft dysfunction(EAD)remains a critical...Liver transplantation(LT)is the standard therapy for individuals afflicted with end-stage liver disease.Despite notable advancements in LT technology,the incidence of early allograft dysfunction(EAD)remains a critical concern,exacerbating the current organ shortage and detrimentally affecting the prognosis of recipients.Unfortunately,the perplexing hepatic heterogeneity has impeded characterization of the cellular traits and molecular events that contribute to EAD.Herein,we constructed a pioneering single-cell transcriptomic landscape of human transplanted livers derived from non-EAD and EAD patients,with 12 liver samples collected from 7 donors during the cold perfusion and portal reperfusion stages.Comparison of the 75231 cells of non-EAD and EAD patients revealed an EAD-associated immune niche comprising mucosal-associated invariant T cells,granzyme B^(+)(GZMB^(+))granzyme K^(+)(GZMK^(+))natural killer cells,and S100 calcium binding protein A12^(+)(S100A12^(+))neutrophils.Moreover,we verified this immune niche and its association with EAD occurrence in two independent cohorts.Our findings elucidate the cellular characteristics of transplanted livers and the EAD-associated pathogenic immune niche at the single-cell level,thus,offering valuable insights into EAD onset.展开更多
Nickel-manganese binary layered oxides with high working potential and low cost are potential candidates for sodium-ion batteries,but their electrochemical properties are highly related to compositional diversity.Dive...Nickel-manganese binary layered oxides with high working potential and low cost are potential candidates for sodium-ion batteries,but their electrochemical properties are highly related to compositional diversity.Diverse composite materials with various phase structures of P3,P2/P3,P2,P2/O3,and P2/P3/O3 were synthesized by manipulating the sodium content and calcination conditions,leading to the construction of a synthetic phase diagram for Na_(x)Ni_(0.25)Mn_(0.75)O_(2)(0.45≤x≤1.1).Then,we compared the electrochemical characteristics and structural evolution during the desodiation/sodiation process of P2,P2/P3,P2/03,and P2/P3/O3-Na_(x)Ni_(0.25)Mn_(0.75)O_(2).Among them,P2/P3-Na0.75Ni0.25Mn0.75O2exhibits the best rate capability of 90.9 mA h g^(-1)at 5 C,with an initial discharge capacity of 142.62 mA h g^(-1)at 0.1 C and a capacity retention rate of 78.25%after 100 cycles at 1 C in the voltage range of 2-4.3 V.The observed superior sodium storage performance of P2/P3 hybrids compared to other composite phases can be attributed to the enhanced Na^(+)transfer dynamic,reduction of the Jahn-teller effect,and improved reaction reversibility induced by the synergistic effect of P2 and P3 phases.The systematic research and exploration of phases in Na_(x)Ni_(0.25)Mn_(0.75)O_(2)provide new sights into high-performance nickel-manganese binary layered oxide for sodium-ion batteries.展开更多
BACKGROUND Ulcerative colitis is a chronic inflammatory disease of the colon with an unknown etiology.Alkaline sphingomyelinase(alk-SMase)is specifically expressed by intestinal epithelial cells,and has been reported ...BACKGROUND Ulcerative colitis is a chronic inflammatory disease of the colon with an unknown etiology.Alkaline sphingomyelinase(alk-SMase)is specifically expressed by intestinal epithelial cells,and has been reported to play an anti-inflammatory role.However,the underlying mechanism is still unclear.AIM To explore the mechanism of alk-SMase anti-inflammatory effects on intestinal barrier function and oxidative stress in dextran sulfate sodium(DSS)-induced colitis.METHODS Mice were administered 3%DSS drinking water,and disease activity index was determined to evaluate the status of colitis.Intestinal permeability was evaluated by gavage administration of fluorescein isothiocyanate dextran,and bacterial translocation was evaluated by measuring serum lipopolysaccharide.Intestinal epithelial cell ultrastructure was observed by electron microscopy.Western blotting and quantitative real-time reverse transcription-polymerase chain reaction were used to detect the expression of intestinal barrier proteins and mRNA,respectively.Serum oxidant and antioxidant marker levels were analyzed using commercial kits to assess oxidative stress levels.RESULTS Compared to wild-type(WT)mice,inflammation and intestinal permeability in alk-SMase knockout(KO)mice were more severe beginning 4 d after DSS induction.The mRNA and protein levels of intestinal barrier proteins,including zonula occludens-1,occludin,claudin-3,claudin-5,claudin-8,mucin 2,and secretory immunoglobulin A,were significantly reduced on 4 d after DSS treatment.Ultrastructural observations revealed progressive damage to the tight junctions of intestinal epithelial cells.Furthermore,by day 4,mitochondria appeared swollen and degenerated.Additionally,compared to WT mice,serum malondialdehyde levels in KO mice were higher,and the antioxidant capacity was significantly lower.The expression of the transcription factor nuclear factor erythroid 2-related factor 2(Nrf2)in the colonic mucosal tissue of KO mice was significantly decreased after DSS treatment.mRNA levels of Nrf2-regulated downstream antioxidant enzymes were also decreased.Finally,colitis in KO mice could be effectively relieved by the injection of tertiary butylhydroquinone,which is an Nrf2 activator.CONCLUSION Alk-SMase regulates the stability of the intestinal mucosal barrier and enhances antioxidant activity through the Nrf2 signaling pathway.展开更多
Background: Delayed gastric emptying(DGE) is one of the most common complications after pancreaticoduodenectomy(PD). DGE represents impaired gastric motility without significant mechanical obstruction and is associate...Background: Delayed gastric emptying(DGE) is one of the most common complications after pancreaticoduodenectomy(PD). DGE represents impaired gastric motility without significant mechanical obstruction and is associated with an increased length of hospital stay, increased healthcare costs, and a high readmission rate. We reviewed published studies on various technical modifications to reduce the incidence of DGE. Data sources: Studies were identified by searching Pub Med for relevant articles published up to December 2022. The following search terms were used: “pancreaticoduodenectomy”, “pancreaticojejunostomy”, “pancreaticogastrostomy”, “gastric emptying”, “gastroparesis” and “postoperative complications”. The search was limited to English publications. Additional articles were identified by a manual search of references from key articles. Results: In recent years, various surgical procedures and techniques have been explored to reduce the incidence of DGE. Pyloric resection, Billroth II reconstruction, Braun's enteroenterostomy, and antecolic reconstruction may be associated with a decreased incidence of DGE, but more high-powered studies are needed in the future. Neither laparoscopic nor robotic surgery has demonstrated superiority in preventing DGE, and the use of staplers is controversial regarding whether they can reduce the incidence of DGE. Conclusions: Despite many innovations in surgical techniques, there is no surgical procedure that is superior to others to reduce DGE. Further larger prospective randomized studies are needed.展开更多
We propose an ansatz without adjustable parameters for the calculation of a dynamical structure factor.The ansatz combines the quasi-particle Green’s function,especially the contribution from the renormalization fact...We propose an ansatz without adjustable parameters for the calculation of a dynamical structure factor.The ansatz combines the quasi-particle Green’s function,especially the contribution from the renormalization factor,and the exchange-correlation kernel from time-dependent density functional theory together,verified for typical metals and semiconductors from a plasmon excitation regime to the Compton scattering regime.It has the capability to reconcile both small-angle and large-angle inelastic x-ray scattering(IXS)signals with muchimproved accuracy,which can be used as the theoretical base model,in inversely inferring electronic structures of condensed matter from IXS experimental signals directly.It may also be used to diagnose thermal parameters,such as temperature and density,of dense plasmas in x-ray Thomson scattering experiments.展开更多
This paper aims to explore how a veteran teacher organizes online teaching initiated by the pandemic and how she deals with the problems in online teacher-student verbal interaction.By analyzing a corpus of 20 audio-r...This paper aims to explore how a veteran teacher organizes online teaching initiated by the pandemic and how she deals with the problems in online teacher-student verbal interaction.By analyzing a corpus of 20 audio-recorded online lessons between a math teacher and her students during the COVID-19 pandemic from April 11 to May 10,2022,four interactional segments are selected as the focus of the study.The results of the conversation analysis of the segments showed that students’modesty,lack of confidence,lack of ability,and network delay are the main factors affecting online teacher-student interaction.By encouraging students to answer questions,enlightening students to give answers,enriching students’answers,and entertaining the teaching atmosphere(“4Es”strategies),the teacher solved the problems successfully.The findings from this study can provide pedagogical experience and implications for practical teaching.展开更多
Dolomites and eruptive rocks are well-developed in the Permian Fengcheng Formation in Junggar Basin in China, in which oil and gas are accumulated extensively. Until now, high-yield industrial oil and gas flows have b...Dolomites and eruptive rocks are well-developed in the Permian Fengcheng Formation in Junggar Basin in China, in which oil and gas are accumulated extensively. Until now, high-yield industrial oil and gas flows have been obtained in the dolomitic tuff of the second unit of the Fengcheng Formation, which demonstrates the huge exploration potential of the thick layer of massive dolomitic tuff. The lithology of the second unit of the Fengcheng Formation in this area has gradually transformed from the dolomite, dolomitic tuff to siltstone from east to west. Moreover, the well testing shows that the reservoir is oil-saturated, and the production rate mainly depends on the reservoir’s physical properties and fracture development. In this study, different types of data including core data, well log and seismic data are used cooperatively to characterize the sedimentary, structure and fracture features of the Fengcheng Formation, and then characterize the promising target zone in the study area. The result indicates that hydrocarbons are most accumulated along the deep fault in the Wu-Xia fault zone, which will be the favorable zone for the next progressive exploration.展开更多
A new flux-based hybrid subcell-remapping algorithm for staggered multimaterial arbitrary Lagrangian-Eulerian (MMALE) methods is presented. This new method is an effective generalization of the original subcell-remapp...A new flux-based hybrid subcell-remapping algorithm for staggered multimaterial arbitrary Lagrangian-Eulerian (MMALE) methods is presented. This new method is an effective generalization of the original subcell-remapping method to the multi-material regime (LOUBERE, R. and SHASHKOV,M. A subcell remapping method on staggered polygonal grids for arbitrary-Lagrangian-Eulerian methods. Journal of Computational Physics, 209, 105–138 (2005)). A complete remapping procedure of all fluid quantities is described detailedly in this paper. In the pure material regions, remapping of mass and internal energy is performed by using the original subcell-remapping method. In the regions near the material interfaces, remapping of mass and internal energy is performed with the intersection-based fluxes where intersections are performed between the swept regions and pure material polygons in the Lagrangian mesh, and an approximate approach is then introduced for constructing the subcell mass fluxes. In remapping of the subcell momentum, the mass fluxes are used to construct the momentum fluxes by multiplying a reconstructed velocity in the swept region. The nodal velocity is then conservatively recovered. Some numerical examples simulated in the full MMALE regime and several purely cyclic remapping examples are presented to prove the properties of the remapping method.展开更多
Saliva testing is a vital method for clinical applications,for its noninvasive features,richness in substances,and the huge amount.Due to its direct anatomical connection with oral,digestive,and endocrine systems,clin...Saliva testing is a vital method for clinical applications,for its noninvasive features,richness in substances,and the huge amount.Due to its direct anatomical connection with oral,digestive,and endocrine systems,clinical usage of saliva testing for these diseases is promising.Furthermore,for other diseases that seeming to have no correlations with saliva,such as neurodegenerative diseases and psychological diseases,researchers also reckon saliva informative.Tremendous papers are being produced in this field.Updated summaries of recent literature give newcomers a shortcut to have a grasp of this topic.Here,we focused on recent research about saliva biomarkers that are derived from humans,not from other organisms.The review mostly addresses the proceedings from 2016 to 2022,to shed light on the promising usage of saliva testing in clinical diagnostics.We recap the recent advances following the category of different types of biomarkers,such as intracellular DNA,RNA,proteins and intercellular exosomes,cell-free DNA,to give a comprehensive impression of saliva biomarker testing.展开更多
After the pursuit of seventy years,the invention of polar codes indicates that we have found the first capacity-achieving coding with low complexity construction and decoding,which is the great breakthrough of the cod...After the pursuit of seventy years,the invention of polar codes indicates that we have found the first capacity-achieving coding with low complexity construction and decoding,which is the great breakthrough of the coding theory in the past two decades.In this survey,we retrospect the history of polar codes and summarize the advancement in the past ten years.First,the primary principle of channel polarization is investigated such that the basic construction,coding method and the classic successive cancellation(SC)decoding are reviewed.Second,in order to improve the performance of the finite code length,we introduce the guiding principle and conclude five design criteria for the construction,design and implementation of the polar code in the practical communication system based on the exemplar schemes in the literature.Especially,we explain the design principle behind the concatenated coding and rate matching of polar codes in 5G wireless system.Furthermore,the improved SC decoding algorithms,such as SC list(SCL)decoding and SC stack(SCS)decoding etc.,are investigated and compared.Finally,the research prospects of polar codes for the future 6G communication system are explored,including the optimization of short polar codes,coding construction in fading channels,polar coded modulation and HARQ,and the polar coded transmission,namely polar processing.Predictably,as a new coding methodology,polar codes will shine a light on communication theory and unveil a revolution in transmission technology.展开更多
Lodging is a critical constraint to yield increase.There appear to be tradeoffs between yield formation and lodging resistance in maize.Hypothetically,it is feasible to reduce lodging risk as well as increase grain yi...Lodging is a critical constraint to yield increase.There appear to be tradeoffs between yield formation and lodging resistance in maize.Hypothetically,it is feasible to reduce lodging risk as well as increase grain yield by optimizing dry-matter allocation to different organs under different environments.A three-year field experiment was conducted using four maize cultivars with differing lodging resistances and five growing environments in 2018–2020.Lodging-susceptible(LS)cultivars on average yielded more than lodging-resistant(LR)cultivars when lodging was not present.The yield components kernel number per ear(KN)and thousand-kernel weight(TKW)were both negatively correlated with lodging resistance traits(stalk bending strength,rind penetration strength,and dry matter weight per internode length).Before silking,the LR cultivar Lishou 1(LS1)transported more assimilates to the basal stem,resulting in a thicker basal stem,which reduced dry matter allocation to the ear and in turn KN.The lower KN of LS1 was also due partly to the lower plant height(PH),which increased lodging resistance but limited plant dry matter production.In contrast,the LS cultivars Xianyu 335(XY335)and Xundan 20(XD20)produced and allocated more photoassimilates to ears,but limited dry matter allocation to stems.After silking,LS cultivars showed higher TKW than LR cultivars as a function of high photoassimilate productivity and high assimilate allocation to the ear.The higher lodging resistance of LS1 was due mainly to the greater assimilate allocation to stem after silking and lower PH and ear height(EH).High-yielding and high-LR traits of Fumin(FM985)were related to optimized EH and stem anatomical structure,higher leaf productivity,low assimilate demand for kernel formation,and assimilate partitioning to ear.A high presilking temperature accelerated stem extension but reduced stem dry matter accumulation and basal stem strength.Post-silking temperature influences lodging resistance and yield more than other environmental factors.These results will be useful in understanding the tradeoffs between KN,KW,and LR in maize and environmental influences on these tradeoffs.展开更多
We report an experimental demonstration of geopotential difference measurement using a pair of transportable ^(40)Ca^(+) optical clocks(TOC-729-1 and TOC-729-3)in the laboratory,each of them has an uncertainty of 1.3&...We report an experimental demonstration of geopotential difference measurement using a pair of transportable ^(40)Ca^(+) optical clocks(TOC-729-1 and TOC-729-3)in the laboratory,each of them has an uncertainty of 1.3×10^(−17) and an instability of 4.8×10^(−15)/√τ.Referenced to a stationary clock of TOC-729-1,the geopotential difference measurements are realized by moving TOC-729-3 to three different locations and the relevant altitude differences are measured with uncertainties at the level of 20 cm.After correcting the systematic shifts(including gravitational red shift),the two-clock frequency difference is measured to be–0.7(2.2)×10^(−17),considering both the statistic(1.0×10^(−17))and the systematic(1.9×10^(−17))uncertainties.The frequency difference between these two clocks is within their respective uncertainties,verifying the reliability of transportable ^(40)Ca^(+) optical clocks at the low level of 10^(−17).展开更多
The problem of three-dimensional(3D) acoustic scattering in a complex medium has aroused considerable interest of researchers for many years. An ultrasonic scattered field calculating technique is proposed to study th...The problem of three-dimensional(3D) acoustic scattering in a complex medium has aroused considerable interest of researchers for many years. An ultrasonic scattered field calculating technique is proposed to study the scattering echo from strongly scattered materials in a two-layer medium in this work. Firstly, with the high frequency stationary phase method,the Green's function of two-layer fluid media is derived. And then based on the idea of integral equation discretization,the Green's function method is extended to two-layer fluid media to derive the scattering field expression of defects in a complex medium. With this method, the scattering field of 3D defect in a two-layer medium is calculated and the characteristics of received echoes are studied. The results show that this method is able to solve the scattering P wave field of 3D defect with arbitrary shape at any scattering intensity in two-layer media. Considering the circumstance of waterimmersion ultrasonic non-destructive test(NDT), the scattering sound field characteristics of different types of defects are analyzed by simulation, which will help to optimize the detection scheme and corresponding imaging method in practice so as to improve the detection quality.展开更多
基金Natural Science Foundation of China(No.51871244)Hunan Provincial Innovation Foundation for Postgraduate,China(No.CX20200172)Fundamental Research Funds for the Central Universities of Central South University,China(No.1053320190103)。
文摘December 9,2022 is the 90th birthday of Tong Zhang,a mathematician in Institute of Mathematics,Chinese Academy of Sciences where he was always working on the Riemann problem for gas dynamics in his mathematical life.To celebrate his 90th birthday and great contributions to this specifc feld,we organize this focused issue in the journal Communications on Applied Mathematics and Computation,since the Riemann problem has been proven to be a building block in all felds of theory,numerics and applications of hyperbolic conservation laws.
基金Beijing Natural Science Foundation,Grant/Award Number:L222145 and L222030Emerging Engineering Interdisciplinary Project and the Fundamental Research Funds for the Central Universities,Grant/Award Number:PKU2022XGK008Peking University Medicine Fund of Fostering Young Scholars’Scientific&Technological Innovation,Grant/Award Number:BMU2022PY010。
文摘Background:Osteoporosis is a chronic bone disease characterized by bone loss and decreased bone strength.However,current anti-resorptive drugs carry a risk of various complications.The deep learning-based efficacy prediction system(DLEPS)is a forecasting tool that can effectively compete in drug screening and prediction based on gene expression changes.This study aimed to explore the protective effect and potential mechanisms of cinobufotalin(CB),a traditional Chinese medicine(TCM),on bone loss.Methods:DLEPS was employed for screening anti-osteoporotic agents according to gene profile changes in primary osteoporosis.Micro-CT,histological and morphological analysis were applied for the bone protective detection of CB,and the osteogenic differentiation/function in human bone marrow mesenchymal stem cells(hBMMSCs)were also investigated.The underlying mechanism was verified using qRT-PCR,Western blot(WB),immunofluorescence(IF),etc.Results:A safe concentration(0.25mg/kg in vivo,0.05μM in vitro)of CB could effectively preserve bone mass in estrogen deficiency-induced bone loss and promote osteogenic differentiation/function of hBMMSCs.Both BMPs/SMAD and Wnt/β-catenin signaling pathways participated in CB-induced osteogenic differentiation,further regulating the expression of osteogenesis-associated factors,and ultimately promoting osteogenesis.Conclusion:Our study demonstrated that CB could significantly reverse estrogen deficiency-induced bone loss,further promoting osteogenic differentiation/function of hBMMSCs,with BMPs/SMAD and Wnt/β-catenin signaling pathways involved.
基金supported by the National Natural Science Foundation of China,No.82071278(to PY)Outstanding Young Medical Talents Project of Changhai Hospital,No.2021JCSQ03(to PY)+1 种基金Shanghai Sailing Program,No.20YF1448000(to XZ)Medical Health Science and Technology Project of Zhoushan City,No.2022JRC01(to HL).
文摘Astrocytes are the most abundant glial cells in the central nervous system;they participate in crucial biological processes,maintain brain structure,and regulate nervous system function.Exosomes are cell-derived extracellular vesicles containing various bioactive molecules including proteins,peptides,nucleotides,and lipids secreted from their cellular sources.Increasing evidence shows that exosomes participate in a communication network in the nervous system,in which astrocyte-derived exosomes play important roles.In this review,we have summarized the effects of exosomes targeting astrocytes and the astrocyte-derived exosomes targeting other cell types in the central nervous system.We also discuss the potential research directions of the exosome-based communication network in the nervous system.The exosome-based intercellular communication focused on astrocytes is of great significance to the biological and/or pathological processes in different conditions in the brain.New strategies may be developed for the diagnosis and treatment of neurological disorders by focusing on astrocytes as the central cells and utilizing exosomes as communication mediators.
基金financially supported by the National Nature Science Foundation of China (32372077)the Project of Chongqing Science and Technology Commission (CSTB2023NSCQ-MSX0355)the Fundamental Research Funds for the Central Universities (SWU120075)。
文摘The necrotrophic fungus, Sclerotinia sclerotiorum, employs an array of cell wall-degrading enzymes(CWDEs), including cellulase, to dismantle host cell walls. However, the molecular mechanisms through which S. sclerotiorum degrades cellulose remain elusive. Here, we unveil a novel secretory cellobiohydrolase, SsdchA, characterized by a signal peptide and a Glyco_hydro_7(GH7) domain. SsdchA exhibits a robust expression of during early infection stages. Interestingly, colony morphology and growth rates remain unaffected across the wild-type, SsdchA deletion strains and SsdchA overexpression strains on potato dextrose agar(PDA) medium. Nevertheless, the pathogenicity and cellobiohydrolase activity decreased in the SsdchA deletion strains, but enhanced in the SsdchA overexpression strains. Moreover,the heterologous expression of SsdchA in Arabidopsis thaliana leads to reduced cellulose content and heightened susceptibility to S. sclerotiorum. Collectively, our data underscore the pivotal role of the novel cellobiohydrolase SsdchA in the pathogenicity of S. sclerotiorum.
基金supported by the National Natural Science Foundation of China(82200725)the Innovation Team and Talents Cultivation Program of National Administration of Traditional Chinese Medicine(ZYYCXTD-D-202002)+4 种基金the Fundamental Research Funds for the Central Universities(226-2023-00114,226-2022-00226,and 226-2023-00059)the Key Program of National Natural Science Foundation of China(81930016)the Key Research and Development Program of China(2021YFA1100500)the Major Research Plan of the National Natural Science Foundation of China(92159202)the Ningbo Top Medical and Health Research Program(2022030309).
文摘Liver transplantation(LT)is the standard therapy for individuals afflicted with end-stage liver disease.Despite notable advancements in LT technology,the incidence of early allograft dysfunction(EAD)remains a critical concern,exacerbating the current organ shortage and detrimentally affecting the prognosis of recipients.Unfortunately,the perplexing hepatic heterogeneity has impeded characterization of the cellular traits and molecular events that contribute to EAD.Herein,we constructed a pioneering single-cell transcriptomic landscape of human transplanted livers derived from non-EAD and EAD patients,with 12 liver samples collected from 7 donors during the cold perfusion and portal reperfusion stages.Comparison of the 75231 cells of non-EAD and EAD patients revealed an EAD-associated immune niche comprising mucosal-associated invariant T cells,granzyme B^(+)(GZMB^(+))granzyme K^(+)(GZMK^(+))natural killer cells,and S100 calcium binding protein A12^(+)(S100A12^(+))neutrophils.Moreover,we verified this immune niche and its association with EAD occurrence in two independent cohorts.Our findings elucidate the cellular characteristics of transplanted livers and the EAD-associated pathogenic immune niche at the single-cell level,thus,offering valuable insights into EAD onset.
基金supported by project from the National Natural Science Foundation of China(21805018)by the Sichuan Science and Technology Program(2022ZHCG0018,2023NSFSC0117,2023ZHCG0060)+1 种基金the Yibin Science and Technology Program(2022JB005)project funded by the China Postdoctoral Science Foundation(2022M722704)。
文摘Nickel-manganese binary layered oxides with high working potential and low cost are potential candidates for sodium-ion batteries,but their electrochemical properties are highly related to compositional diversity.Diverse composite materials with various phase structures of P3,P2/P3,P2,P2/O3,and P2/P3/O3 were synthesized by manipulating the sodium content and calcination conditions,leading to the construction of a synthetic phase diagram for Na_(x)Ni_(0.25)Mn_(0.75)O_(2)(0.45≤x≤1.1).Then,we compared the electrochemical characteristics and structural evolution during the desodiation/sodiation process of P2,P2/P3,P2/03,and P2/P3/O3-Na_(x)Ni_(0.25)Mn_(0.75)O_(2).Among them,P2/P3-Na0.75Ni0.25Mn0.75O2exhibits the best rate capability of 90.9 mA h g^(-1)at 5 C,with an initial discharge capacity of 142.62 mA h g^(-1)at 0.1 C and a capacity retention rate of 78.25%after 100 cycles at 1 C in the voltage range of 2-4.3 V.The observed superior sodium storage performance of P2/P3 hybrids compared to other composite phases can be attributed to the enhanced Na^(+)transfer dynamic,reduction of the Jahn-teller effect,and improved reaction reversibility induced by the synergistic effect of P2 and P3 phases.The systematic research and exploration of phases in Na_(x)Ni_(0.25)Mn_(0.75)O_(2)provide new sights into high-performance nickel-manganese binary layered oxide for sodium-ion batteries.
基金the Natural Science Foundation of Hainan Province,No.823MS046the Talent Program of Hainan Medical University,No.XRC2022007.
文摘BACKGROUND Ulcerative colitis is a chronic inflammatory disease of the colon with an unknown etiology.Alkaline sphingomyelinase(alk-SMase)is specifically expressed by intestinal epithelial cells,and has been reported to play an anti-inflammatory role.However,the underlying mechanism is still unclear.AIM To explore the mechanism of alk-SMase anti-inflammatory effects on intestinal barrier function and oxidative stress in dextran sulfate sodium(DSS)-induced colitis.METHODS Mice were administered 3%DSS drinking water,and disease activity index was determined to evaluate the status of colitis.Intestinal permeability was evaluated by gavage administration of fluorescein isothiocyanate dextran,and bacterial translocation was evaluated by measuring serum lipopolysaccharide.Intestinal epithelial cell ultrastructure was observed by electron microscopy.Western blotting and quantitative real-time reverse transcription-polymerase chain reaction were used to detect the expression of intestinal barrier proteins and mRNA,respectively.Serum oxidant and antioxidant marker levels were analyzed using commercial kits to assess oxidative stress levels.RESULTS Compared to wild-type(WT)mice,inflammation and intestinal permeability in alk-SMase knockout(KO)mice were more severe beginning 4 d after DSS induction.The mRNA and protein levels of intestinal barrier proteins,including zonula occludens-1,occludin,claudin-3,claudin-5,claudin-8,mucin 2,and secretory immunoglobulin A,were significantly reduced on 4 d after DSS treatment.Ultrastructural observations revealed progressive damage to the tight junctions of intestinal epithelial cells.Furthermore,by day 4,mitochondria appeared swollen and degenerated.Additionally,compared to WT mice,serum malondialdehyde levels in KO mice were higher,and the antioxidant capacity was significantly lower.The expression of the transcription factor nuclear factor erythroid 2-related factor 2(Nrf2)in the colonic mucosal tissue of KO mice was significantly decreased after DSS treatment.mRNA levels of Nrf2-regulated downstream antioxidant enzymes were also decreased.Finally,colitis in KO mice could be effectively relieved by the injection of tertiary butylhydroquinone,which is an Nrf2 activator.CONCLUSION Alk-SMase regulates the stability of the intestinal mucosal barrier and enhances antioxidant activity through the Nrf2 signaling pathway.
文摘Background: Delayed gastric emptying(DGE) is one of the most common complications after pancreaticoduodenectomy(PD). DGE represents impaired gastric motility without significant mechanical obstruction and is associated with an increased length of hospital stay, increased healthcare costs, and a high readmission rate. We reviewed published studies on various technical modifications to reduce the incidence of DGE. Data sources: Studies were identified by searching Pub Med for relevant articles published up to December 2022. The following search terms were used: “pancreaticoduodenectomy”, “pancreaticojejunostomy”, “pancreaticogastrostomy”, “gastric emptying”, “gastroparesis” and “postoperative complications”. The search was limited to English publications. Additional articles were identified by a manual search of references from key articles. Results: In recent years, various surgical procedures and techniques have been explored to reduce the incidence of DGE. Pyloric resection, Billroth II reconstruction, Braun's enteroenterostomy, and antecolic reconstruction may be associated with a decreased incidence of DGE, but more high-powered studies are needed in the future. Neither laparoscopic nor robotic surgery has demonstrated superiority in preventing DGE, and the use of staplers is controversial regarding whether they can reduce the incidence of DGE. Conclusions: Despite many innovations in surgical techniques, there is no surgical procedure that is superior to others to reduce DGE. Further larger prospective randomized studies are needed.
基金supported by the National Natural Science Foundation of China(Grant Nos.12375234,12005012,and U1930402)the Laboratory Youth Fund of Institute of Applied Physics and Computational Mathematics(Grant No.6142A05QN21005)。
文摘We propose an ansatz without adjustable parameters for the calculation of a dynamical structure factor.The ansatz combines the quasi-particle Green’s function,especially the contribution from the renormalization factor,and the exchange-correlation kernel from time-dependent density functional theory together,verified for typical metals and semiconductors from a plasmon excitation regime to the Compton scattering regime.It has the capability to reconcile both small-angle and large-angle inelastic x-ray scattering(IXS)signals with muchimproved accuracy,which can be used as the theoretical base model,in inversely inferring electronic structures of condensed matter from IXS experimental signals directly.It may also be used to diagnose thermal parameters,such as temperature and density,of dense plasmas in x-ray Thomson scattering experiments.
文摘This paper aims to explore how a veteran teacher organizes online teaching initiated by the pandemic and how she deals with the problems in online teacher-student verbal interaction.By analyzing a corpus of 20 audio-recorded online lessons between a math teacher and her students during the COVID-19 pandemic from April 11 to May 10,2022,four interactional segments are selected as the focus of the study.The results of the conversation analysis of the segments showed that students’modesty,lack of confidence,lack of ability,and network delay are the main factors affecting online teacher-student interaction.By encouraging students to answer questions,enlightening students to give answers,enriching students’answers,and entertaining the teaching atmosphere(“4Es”strategies),the teacher solved the problems successfully.The findings from this study can provide pedagogical experience and implications for practical teaching.
文摘Dolomites and eruptive rocks are well-developed in the Permian Fengcheng Formation in Junggar Basin in China, in which oil and gas are accumulated extensively. Until now, high-yield industrial oil and gas flows have been obtained in the dolomitic tuff of the second unit of the Fengcheng Formation, which demonstrates the huge exploration potential of the thick layer of massive dolomitic tuff. The lithology of the second unit of the Fengcheng Formation in this area has gradually transformed from the dolomite, dolomitic tuff to siltstone from east to west. Moreover, the well testing shows that the reservoir is oil-saturated, and the production rate mainly depends on the reservoir’s physical properties and fracture development. In this study, different types of data including core data, well log and seismic data are used cooperatively to characterize the sedimentary, structure and fracture features of the Fengcheng Formation, and then characterize the promising target zone in the study area. The result indicates that hydrocarbons are most accumulated along the deep fault in the Wu-Xia fault zone, which will be the favorable zone for the next progressive exploration.
基金supported by the National Natural Science Foundation of China(81973690 and 81904049)Experimental Technology Standardization Research Project of Beijing University of Chinese Medicine(2021-SYJS-001).
基金Project supported by the China Postdoctoral Science Foundation(No.2017M610823)
文摘A new flux-based hybrid subcell-remapping algorithm for staggered multimaterial arbitrary Lagrangian-Eulerian (MMALE) methods is presented. This new method is an effective generalization of the original subcell-remapping method to the multi-material regime (LOUBERE, R. and SHASHKOV,M. A subcell remapping method on staggered polygonal grids for arbitrary-Lagrangian-Eulerian methods. Journal of Computational Physics, 209, 105–138 (2005)). A complete remapping procedure of all fluid quantities is described detailedly in this paper. In the pure material regions, remapping of mass and internal energy is performed by using the original subcell-remapping method. In the regions near the material interfaces, remapping of mass and internal energy is performed with the intersection-based fluxes where intersections are performed between the swept regions and pure material polygons in the Lagrangian mesh, and an approximate approach is then introduced for constructing the subcell mass fluxes. In remapping of the subcell momentum, the mass fluxes are used to construct the momentum fluxes by multiplying a reconstructed velocity in the swept region. The nodal velocity is then conservatively recovered. Some numerical examples simulated in the full MMALE regime and several purely cyclic remapping examples are presented to prove the properties of the remapping method.
基金funded by the National Natural Science Foundation of China(U20A20394,81870754,82272416,82202614)State Key Laboratory of Oral Diseases(2022KXK0402)Clinical and Translational Medicine Research Fund of Chinese Academy of Medical Sciences(2020-I2M-C&T-B-097)。
文摘Saliva testing is a vital method for clinical applications,for its noninvasive features,richness in substances,and the huge amount.Due to its direct anatomical connection with oral,digestive,and endocrine systems,clinical usage of saliva testing for these diseases is promising.Furthermore,for other diseases that seeming to have no correlations with saliva,such as neurodegenerative diseases and psychological diseases,researchers also reckon saliva informative.Tremendous papers are being produced in this field.Updated summaries of recent literature give newcomers a shortcut to have a grasp of this topic.Here,we focused on recent research about saliva biomarkers that are derived from humans,not from other organisms.The review mostly addresses the proceedings from 2016 to 2022,to shed light on the promising usage of saliva testing in clinical diagnostics.We recap the recent advances following the category of different types of biomarkers,such as intracellular DNA,RNA,proteins and intercellular exosomes,cell-free DNA,to give a comprehensive impression of saliva biomarker testing.
基金supported in part by the Key Program of National Natural Science Foundation of China (No.92067202)in part by the National Natural Science Foundation of China (No.62071058)in part by the Major Key Project of PCL (PCL2021A15)。
文摘After the pursuit of seventy years,the invention of polar codes indicates that we have found the first capacity-achieving coding with low complexity construction and decoding,which is the great breakthrough of the coding theory in the past two decades.In this survey,we retrospect the history of polar codes and summarize the advancement in the past ten years.First,the primary principle of channel polarization is investigated such that the basic construction,coding method and the classic successive cancellation(SC)decoding are reviewed.Second,in order to improve the performance of the finite code length,we introduce the guiding principle and conclude five design criteria for the construction,design and implementation of the polar code in the practical communication system based on the exemplar schemes in the literature.Especially,we explain the design principle behind the concatenated coding and rate matching of polar codes in 5G wireless system.Furthermore,the improved SC decoding algorithms,such as SC list(SCL)decoding and SC stack(SCS)decoding etc.,are investigated and compared.Finally,the research prospects of polar codes for the future 6G communication system are explored,including the optimization of short polar codes,coding construction in fading channels,polar coded modulation and HARQ,and the polar coded transmission,namely polar processing.Predictably,as a new coding methodology,polar codes will shine a light on communication theory and unveil a revolution in transmission technology.
基金supported by the project of National Key Research and Development Program of China(2016YFD0300301 and 2017YFD0300603)The 2115 Talent Development Program of China Agricultural University。
文摘Lodging is a critical constraint to yield increase.There appear to be tradeoffs between yield formation and lodging resistance in maize.Hypothetically,it is feasible to reduce lodging risk as well as increase grain yield by optimizing dry-matter allocation to different organs under different environments.A three-year field experiment was conducted using four maize cultivars with differing lodging resistances and five growing environments in 2018–2020.Lodging-susceptible(LS)cultivars on average yielded more than lodging-resistant(LR)cultivars when lodging was not present.The yield components kernel number per ear(KN)and thousand-kernel weight(TKW)were both negatively correlated with lodging resistance traits(stalk bending strength,rind penetration strength,and dry matter weight per internode length).Before silking,the LR cultivar Lishou 1(LS1)transported more assimilates to the basal stem,resulting in a thicker basal stem,which reduced dry matter allocation to the ear and in turn KN.The lower KN of LS1 was also due partly to the lower plant height(PH),which increased lodging resistance but limited plant dry matter production.In contrast,the LS cultivars Xianyu 335(XY335)and Xundan 20(XD20)produced and allocated more photoassimilates to ears,but limited dry matter allocation to stems.After silking,LS cultivars showed higher TKW than LR cultivars as a function of high photoassimilate productivity and high assimilate allocation to the ear.The higher lodging resistance of LS1 was due mainly to the greater assimilate allocation to stem after silking and lower PH and ear height(EH).High-yielding and high-LR traits of Fumin(FM985)were related to optimized EH and stem anatomical structure,higher leaf productivity,low assimilate demand for kernel formation,and assimilate partitioning to ear.A high presilking temperature accelerated stem extension but reduced stem dry matter accumulation and basal stem strength.Post-silking temperature influences lodging resistance and yield more than other environmental factors.These results will be useful in understanding the tradeoffs between KN,KW,and LR in maize and environmental influences on these tradeoffs.
基金Project supported by the Basic Frontier Science Research Program of Chinese Academy of Sciences (Grant No.ZDBS-LY-DQC028)the National Key Research and Development Program of China (Grant No.2017YFA0304404)the National Natural Science Foundation of China (Grant No.11674357)。
文摘We report an experimental demonstration of geopotential difference measurement using a pair of transportable ^(40)Ca^(+) optical clocks(TOC-729-1 and TOC-729-3)in the laboratory,each of them has an uncertainty of 1.3×10^(−17) and an instability of 4.8×10^(−15)/√τ.Referenced to a stationary clock of TOC-729-1,the geopotential difference measurements are realized by moving TOC-729-3 to three different locations and the relevant altitude differences are measured with uncertainties at the level of 20 cm.After correcting the systematic shifts(including gravitational red shift),the two-clock frequency difference is measured to be–0.7(2.2)×10^(−17),considering both the statistic(1.0×10^(−17))and the systematic(1.9×10^(−17))uncertainties.The frequency difference between these two clocks is within their respective uncertainties,verifying the reliability of transportable ^(40)Ca^(+) optical clocks at the low level of 10^(−17).
基金Project supported by the Key Research Program of Frontier Sciences, Chinese Academy of Sciences (Grant No. ZDBS-LY-7023)。
文摘The problem of three-dimensional(3D) acoustic scattering in a complex medium has aroused considerable interest of researchers for many years. An ultrasonic scattered field calculating technique is proposed to study the scattering echo from strongly scattered materials in a two-layer medium in this work. Firstly, with the high frequency stationary phase method,the Green's function of two-layer fluid media is derived. And then based on the idea of integral equation discretization,the Green's function method is extended to two-layer fluid media to derive the scattering field expression of defects in a complex medium. With this method, the scattering field of 3D defect in a two-layer medium is calculated and the characteristics of received echoes are studied. The results show that this method is able to solve the scattering P wave field of 3D defect with arbitrary shape at any scattering intensity in two-layer media. Considering the circumstance of waterimmersion ultrasonic non-destructive test(NDT), the scattering sound field characteristics of different types of defects are analyzed by simulation, which will help to optimize the detection scheme and corresponding imaging method in practice so as to improve the detection quality.
