Psychometric properties of the Chinese version of the Infertility Self-Efficacy Scale

Objective:To translate the English version of Infertility Self-Efficacy Scale into Chinese and to validate the psychometric properties of the Chinese version of the Infertility Self-Efficacy Scale(CISE).Method(s):Participants were recruited from the Gynecology Department of two city hospitals.Five main processes were involved in the formation of CISE[1]:scale translation based on expert consultation[2];pre-test questionnaire development with infertile women's feedback(N=20)[3];factor structure assessed by exploratory and confirmatory factor analysis(N=177)[4];assessment of reliability by internal consistency(N=177)and test-retest reliability(N=21);and[5]assessment of convergent validity with Self-rating Anxiety Scale,Self-rating Depression Scale,and Simplified Coping Style Questionnaire(N=177).Results:This study established a 16-item CISE.Factor analyses confirmed a onecomponent solution,which explained 54.59% of total variances and showed an acceptable model fit.Cronbach's a and test-retest correlation coefficients for the scale were 0.94 and 0.84,respectively.The CISE score was significantly correlated with anxiety(r=0.47),depression(r=0.60),positive coping style(r=0.37),and certain negative coping style items.Conclusion:This 16-item CISE is a reliable and valid measure to evaluate perceived selfefficacy among a sample of Chinese women who underwent infertility treatment.

Study on the Role of Vitamin D in Systemic Lupus Erythematosus

Vitamin D is a hormone precursor with multiple biological effects.It binds to vitamin D receptors on target cells.It is an important participant in the metabolism of calcium and phosphorus in vivo.It is closely related to cell cycle,cell proliferation,differentiation,apoptosis,signal transduction and immune regulation.Its role in the treatment of infection,tumor and even immune diseases has been gradually recognized and studied.Patients with systemic lupus erythematosus generally have decreased levels of active vitamin D,and low levels of vitamin D are associated with disease occurrence,disease activity and complications.In the past ten years,a large number of studies have been carried out on it globally to explore the role of vitamin D in the occurrence and development of systemic lupus erythematosus.This paper summarizes its recent research progress.

Research Progress of Vitamin D and Pathogenesis of Bronchial Asthma

At present the incidence of bronchial asthma on the rise,its pathogenesis and the genetic immune and the relationship between social environment and other aspects are inseparable the activity of vitamin D(Vit D)in the body in the form of 1,25-(OH)2 d3,mainly involved in bone metabolism and calcium absorption in addition to this,a growing number of studies show that in Vit D plays an important role in the pathogenesis of bronchial asthma,play a role in the immune function of bronchial asthma growth hormone sensitivity adjustment and airway remodeling in this paper,the development of a variety of mechanisms,such as Vit D.Review the possible mechanisms affecting bronchial asthma,hoping to provide adjuvant treatment for patients with bronchial asthma,discover new treatment approaches,and improve the quality of life for patients.

Research Progress of Vitamin D and Autoimmune Diseases

As a fat-soluble vitamin,Vitamin D is a necessary hormone to maintain normal physiological activities of the body.In recent years,vitamin D has been considered as a new neuroendocrine-immunomodulatory hormone,and researchers have paid more attention to the study of immune regulatory mechanism.It is not only related to calcium and phosphorus metabolism,bone metabolism and other important metabolic mechanisms of the body,but also closely related to the immune regulation mechanism of the body.Vitamin D deficiency caused by many factors can play a certain role in the development of autoimmune diseases.In this paper,the related mechanisms of vitamin D affecting autoimmune diseases were reviewed,with a view to expound the close correlation between vitamin D and autoimmune diseases,so as to find new diagnosis and treatment approaches for clinical autoimmune diseases and improve the quality of life of patients with autoimmune diseases.

Effect of Metformin on Lactate Metabolism in Normal Hepatocytes under High Glucose Stress in Vitro

To study the effect of metformin on lactate metabolism in hepatocytes in vitro under high glucose stress.Method:LO2 hepatocytes was cultured in vitro,hepatocytes were randomly divided into blank control group,25 mmol/L glucose solution,27 mmol/L glucose solution,29 mmol/L glucose solution,31 mmol/L glucose solution,33 mmol/L glucose solution,35 mmol/L glucose solution treatment group,after determining the optimal concentration as 31 mmol/L,use 30 mmol/L metformin solution,and then divided into blank control group,normal hepatocytes+the optimal concentration of glucose solution,normal hepatocytes+metformin solution,normal hepatocytes+.The optimal concentration of glucose solution normal hepatocytes+metformin solution,calculate the number of hepatocytes on cell count plate respectively in the 12 h,24 h,48 h,and use the lactic acid kit to determine the lactic acid value of the cell culture medium of normal liver cells+optimal concentration glucose solution and normal liver cells+optimal concentration glucose solution+metformin solution at 12 h,24 h,and 48 h,respectively.Results:There was no significant change in the lactic acid concentration but significant increase in the number of surviving hepatocytes in the high-glycemic control group compared with that in the high-glycemic control group without metformin.Conclusions:Metformin has no significant effect on lactic acid metabolism of hepatocytes under high glucose stress in vitro,and has a protective effect on hepatocytes under high glucose stress.Based on this,it is preliminarily believed that metformin is not the direct factor leading to diabetic lactic acidosis.

A Research on the Relationship between Intestinal Flora and Human Longevity

The exploration of human life and health is advancing with the changes of the times.With the growth of age,the occurrence of chronic diseases of human immunity and organ system is frequent,which has a serious impact on human health.Genes,environment and other random factors determine the outcome of longevity,and intestinal flora is considered to be a decisive factor affecting human health and longevity,mainly because of its huge impact on human immunity,growth and development.The study of the relationship between intestinal flora and longevity is beneficial to improve the health status of the elderly and improve the overall life level of human beings,which has great scientific research value.This review will review the role of intestinal flora in longevity.

Analysis of the Association between Intestinal Microflora and Long-lived Elderly People

The intestinal microbiota is the cornerstone of the human intestinal microecosystem and plays an unnegligible role in the growth and health maintenance of the human body.In recent years,many studies have been committed to exploring the potential connection of gut flora and the elderly population.The changes of gut flora are affected by various factors such as age increase,disease,medication,living habits,nutritional structure,and the intestinal flora is expected to be applied to the comprehensive evaluation of elderly health and longevity in the future.Based on this,the research progress of the general elderly and its related influencing factors.

Metformin and Lactic Acidosis in Diabetic Patients

Metfommin is the basic drug in the clinical treatment of Diabetes,often used in the treatment of Type 2 Diabetes Mellitus(T2DM).Its effect has been fully verificd in the clinical treatment of T2DM.However,in the treatment of T2DM with metformin,there is still a certain probability of related lactic acidosis,and the fatality rate is high.Therefore,is the use of metformin drug treatment a direct risk factor for lactic acidosis in diabetic patients?This paper will review the hypoglycemic mechanism of metformin and related studies on lactic acidosis,so as to further explore the relationship between metformin and lactic acidosis in diabetic patients,and provide help and reference for metformin drugs in the clinical treatment ofT2DM.

Research Progress on the Relationship between Polymorphism and SLE of Vitamin D Metabolic Pathway Related Gene

Vitamin D is a class of hormones necessary to maintain normal physiological activities of the body.A large number of studies have shown that vitamin D,as a fat-soluble vitamin,is not only related to calcium and phosphorus metabolism,but also closely related to immune regulation,humoral regulation,cell cycle and so on.Systemic Lupus erythema-Tosus(SLE)is a specific autoimmune diffuse connective tissue disease that causes tissue and organ damage under the joint action of multiple factors such as environment and heredity.Among many factors,the vitamin D metabolism pathway gene is particularly important for its influence.Some literature has shown that the genetic polymorphism of vitamin D metabolic pathway genes is correlated with SLE.Therefore,by referring to relevant literature,this paper summarized the progress in the research on the mechanism of genetic polymorphism of vitamin metabolism pathway genes and the development of SLE.

Effect of Metformin on Lactate Metabolism in Normal Hepatocytes under High Glucose Stress in Vitro

Objective To study the effect of metformin on lactate metabolism in hepatocytes in vitro under high glucose stress.In vitro LO2 cells,liver cells were randomly divided into blank control group,25 tendency/L glucose solution,27 tendency/L glucose solution,29 tendency/L glucose solution,31 tendency/L glucose solution,33 tendency/L glucose solution,35 tendency/L glucose solution treatment group,the optimal concentation of 31 tendency after L,use 30 tendency for L metformin solution,and then divided into blank control group,the optimal concentration of glucose solution,normal liver cells+metformin solution normal liver cells.The optimal concentration of glucose solution normal liver cells+metformin solution respectively in the 12h,24 h,48 h on cell count plate to calculate the mumber of liver cells,and using lactic acid determination kit the optimal concentration of glucose solution+normal liver cells and normal liver cells+the optimal concentration of glucose solution+metformin solution respectively in the 12 h,24 h,48 h of cell cultures of lactic acid value.There was no significant change in the lactic acid concentration but significant increase in the number of suviving hepatocytes in the high-glycemic control group compared with that in the high-glycemic control group without metformin.Metformin has no significant effect on lactic acid metabolism of hepatocytes under high glucose stess in vito,and has a protective effect on hepatocytes under high glucose stress.Based on this,it is preliminanily believed that metformin is not the direct factor leading to diabetic lactic acidosis.

Prevalence of HIV Indeterminate Western Blot Tests and Follow-up of HIV Antibody Sero-Conversion in Southeastern China

HIV-indeterminate Western blotting(WB)results are typically obtained in WB confirmatory assays,and the number of indeterminate samples may increase with the detection of HIV infections,which will present considerable challenges for the management of HIV/AIDS.Nucleic acid detection has been used as a laboratory test for screening suspected or indeterminate samples.However,the effectiveness of these assays for the differential diagnosis of HIV-indeterminate WB samples remained undetermined.In this study,210 subjects with HIV-indeterminate WB results were detected from 6360 positive HIV screening samples between 2015 and 2016 in southeastern China,in which HIV-indeterminate WB results accounted for 3.30%.The highest proportion of indeterminate results was observed in pregnant and lying-in women receiving physical examinations(16.67%),followed by that in voluntary blood donors(8.82%).The most common WB band patterns were p24,gpl60 and p24,and gpl60.The follow-up study revealed that the highest negative and positive conversion rates of HIV antibodies were in samples with a single p24 band(80.28%),and with gpl60 and p24 bands(86.21%),respectively.Among the Env,Gag,and Pol antibodies,samples with a Gag band showed the highest negative conversion rate(81.25%),whereas the highest positive conversion rate was observed in samples with an Env band(56.76%).In addition,quantitative and qualitative HIV nucleic acid testing exhibited the highest sensitivity(96.3%)and specificity(97.85%),respectively.Our results indicate a lower proportion of HIV indeterminate WB results in southeastern China compared to previous reports,and the follow-up re-examination of patients with HIV indeterminate results should be performed.Nucleic acid testing facilitates the identification of HIV infections.

Recent advances in pharmaceuticals and personal care products in the surface water and sediments in China

Pharmaceuticals and personal care products （PPCPs） have been regarded as an emerging problem in the surface water environment in the past few decades. In China, although related studies were initiated several years ago, an increasing number of studies on this topic have been conducted in recent years. These studies have expanded knowledge of their occurrence, behavior and associated risk in the surface water environment in China. This review compiles the most recent literature related to the studies of PPCPs in the surface water environment in China. It includes PPCP occurrence in surface water and sediments, their geographical distribution, and outcomes of the associated risk assessment. It shows that antibiotics have received much more attention in both surface water and sediments than other PPCPs. Compared to other countries; most antibiotics in the collected sediments in China showed higher contamination levels. Many more study areas have been covered in recent years; however, attention has been given to only specific areas. Environmental risk assessment based on risk quotients indicated that sulfamethoxazole presents the most significant environmental risk to relevant aquatic organisms; followed by ofloxacin, ciprofloxacin, enrofloxacin, 17α-ethynylestradiol, ibuprofen and diclofenac. Despite limited research on the environmental risk assessment of PPCPs in sediments, higher risks posed by PPCPs in the sediments rather than surfhce water were identified highlighting the need for further risk assessment of PPCPs in sediment samples.

Biodegradation of bezafibrate by the activated sludge under aerobic condition:Effect of initial concentration,temperature and pH

Lab-scale experiments were conducted to investigate the effect of initial concentration,temperature and pH on the removal of bezafibrate(BF)by activated sludge under aerobic condition.The results showed that adsorption of BF onto activated sludge was negligible,and biodegradation was the main removal mechanism of BF.The removal of BF in the aqueous phase by the activated sludge can be described by a pseudo-first-order reaction.The reaction rate constants had a negative relationship with the initial concentration of BF,and dramatically reduced from 0.050 to 0.007 h^-1,when the temperature dropped from 20℃to 10℃.Variation of pH between 5.0 and 9.0 did not have significant influence on the removal of BF,indicating a high adaptation of microorganism in the activated sludge responsible for BF degradation to a wide pH range.The findings of this study are helpful to improve the removal of pharmaceuticals during the wastewater treatment plants by selecting the appropriate process variables,and eventually eliminate their release to the environment.