Dermatophagoides farinae group 2 (Der f2) is a highly polymorphic allergen that shows a distinct pattern of sequence divergence. The effect of the variants on antibody and T cell responses has not been compared. The a...Dermatophagoides farinae group 2 (Der f2) is a highly polymorphic allergen that shows a distinct pattern of sequence divergence. The effect of the variants on antibody and T cell responses has not been compared. The aim of the present study was to evaluate IgE binding, transcription and translations for IL-5, IFN-γ and TGF-β induced by mite allergens. Sera from 24 HDM-allergic patients and 20 non-allergic subjects were measured for IgE reactivity by ELISA. PBMC was cultured with mite allergens (Df, rDerf2) and mitogen (PHA). The supernatants and cell pellet obtained were evaluated for cytokine production by ELISA and cytokine gene expression by RT-PCR, respectively. Four patients showed IgE reactivity to both allergens. Five patients showed IgE reactivity to Df. Other allergic patients and all non-allergic subjects did not show IgE reactivity to mite allergens. Both allergens showed similar levels of IL-5 and IFN-γ transcriptions in allergic patients and non-allergic subjects. The rDer f2 induced IL-5 protein from allergic patients higher than non-allergic subjects, while Df showed IL-5 protein from allergic patients similar to non-allergic subjects. Df induced IFN-protein from allergic patients higher than non-allergic subjects whereas rDer f2 induced IFN-protein from allergic patients similar to non-allergic subjects. The ratio of IFN-γ to IL-5 production after stimulation with rDer f2 was higher in non-allergic subjects than in allergic patients. Our data demonstrated that the changes in the sequence of rDer f2 compared with native Df had effect on cytokine production in both allergic patients and non-allergic subjects.展开更多
文摘Dermatophagoides farinae group 2 (Der f2) is a highly polymorphic allergen that shows a distinct pattern of sequence divergence. The effect of the variants on antibody and T cell responses has not been compared. The aim of the present study was to evaluate IgE binding, transcription and translations for IL-5, IFN-γ and TGF-β induced by mite allergens. Sera from 24 HDM-allergic patients and 20 non-allergic subjects were measured for IgE reactivity by ELISA. PBMC was cultured with mite allergens (Df, rDerf2) and mitogen (PHA). The supernatants and cell pellet obtained were evaluated for cytokine production by ELISA and cytokine gene expression by RT-PCR, respectively. Four patients showed IgE reactivity to both allergens. Five patients showed IgE reactivity to Df. Other allergic patients and all non-allergic subjects did not show IgE reactivity to mite allergens. Both allergens showed similar levels of IL-5 and IFN-γ transcriptions in allergic patients and non-allergic subjects. The rDer f2 induced IL-5 protein from allergic patients higher than non-allergic subjects, while Df showed IL-5 protein from allergic patients similar to non-allergic subjects. Df induced IFN-protein from allergic patients higher than non-allergic subjects whereas rDer f2 induced IFN-protein from allergic patients similar to non-allergic subjects. The ratio of IFN-γ to IL-5 production after stimulation with rDer f2 was higher in non-allergic subjects than in allergic patients. Our data demonstrated that the changes in the sequence of rDer f2 compared with native Df had effect on cytokine production in both allergic patients and non-allergic subjects.
