Objective:To investigate the prevalence of non-tuberculosis mycobacteria(NTM)among the samples deposited from the National Tuberculosis Reference Laboratory of Iran between 2011 and 2018.Methods:The study evaluated th...Objective:To investigate the prevalence of non-tuberculosis mycobacteria(NTM)among the samples deposited from the National Tuberculosis Reference Laboratory of Iran between 2011 and 2018.Methods:The study evaluated the prevalence of NTM among specimens from patients with pulmonary tuberculosis symptoms(n=15771)deposited at the National Tuberculosis Reference Laboratory of Iran from 2011 to 2018.Detection of Mycobacterium(M.)tuberculosis was based on presence of a 190-bp amplicon from IS6110 insertion sequence using Tb1 and Tb2 primers,and amplicon-negative specimens were tested for NTM and M.tuberculosis(refractory to IS6110 amplification)using restriction fragment length polymorphism PCR of hsp65 amplicon fragment.Results:A total of 7307(46.33%)M.tuberculosis and 658(4.17%)NTM specimens were found,the latter mainly comprising M.abscessus(10.18%),M.avium(2.28%),M.chelonae(8.97%),M.intracellulare(10.49%),M.kansasii(4.71%),and M.simiae(56.08%).Conclusions:As treatment for NTM differs from that for M.tuberculosis,accurate detection of Mycobacterium sp.is of public health significance.展开更多
基金supported by funding from the Mycobacteriology Research Center,National Tuberculosis and Lung Research Institute(NRITLD),Masih Daneshvari Hospital(Grant No.16597)
文摘Objective:To investigate the prevalence of non-tuberculosis mycobacteria(NTM)among the samples deposited from the National Tuberculosis Reference Laboratory of Iran between 2011 and 2018.Methods:The study evaluated the prevalence of NTM among specimens from patients with pulmonary tuberculosis symptoms(n=15771)deposited at the National Tuberculosis Reference Laboratory of Iran from 2011 to 2018.Detection of Mycobacterium(M.)tuberculosis was based on presence of a 190-bp amplicon from IS6110 insertion sequence using Tb1 and Tb2 primers,and amplicon-negative specimens were tested for NTM and M.tuberculosis(refractory to IS6110 amplification)using restriction fragment length polymorphism PCR of hsp65 amplicon fragment.Results:A total of 7307(46.33%)M.tuberculosis and 658(4.17%)NTM specimens were found,the latter mainly comprising M.abscessus(10.18%),M.avium(2.28%),M.chelonae(8.97%),M.intracellulare(10.49%),M.kansasii(4.71%),and M.simiae(56.08%).Conclusions:As treatment for NTM differs from that for M.tuberculosis,accurate detection of Mycobacterium sp.is of public health significance.
