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通过胚胎拯救将野生稻耐旱特性转移到栽培稻进行远缘杂交初探 被引量:6
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作者 迟全勃 prapa sripichitt 许红春 《中国农学通报》 CSCD 2005年第10期368-372,共5页
众所周知,农业用水的缺乏严重影响着世界干旱地区农业的发展,尤其影响着水稻的生产。育出具有耐旱特性的水稻品种从而经济地利用水分是一个重要的课题。前人的研究表明野生稻Oryza属中有许多种具有耐旱性,如果水稻栽培种也具有耐旱性,... 众所周知,农业用水的缺乏严重影响着世界干旱地区农业的发展,尤其影响着水稻的生产。育出具有耐旱特性的水稻品种从而经济地利用水分是一个重要的课题。前人的研究表明野生稻Oryza属中有许多种具有耐旱性,如果水稻栽培种也具有耐旱性,那将会更能适应环境节约用水,大大地提高水稻的收获面积及适应性。Oryzameridionalis和Oryzaaustraliensis具有耐旱特性,试验旨在通过远缘杂交将野生稻Oryzameridionalis和Oryzaaustraliensis的耐旱特性转移到栽培稻OryzaSativa。用Oryzameridionalis花粉对Oryzasativa共2419朵小花进行杂交授粉,并对杂交获得的18粒F1代未成熟种子进行了胚胎拯救,最终仅有一粒F1种子发芽并发育成植株,并获得一粒F2代种子,其杂交率为0.46%,萌芽率为50%,F1代杂种的结实率为0.024%;用Oryzaaustraliensis花粉对Oryzasativa共1848朵小花进行杂交授粉,并对杂交获得的48粒F1代未成熟种子进行了胚胎拯救,有11粒F1种子发芽并发育成植株,全部不育,其杂交率为0.60%,萌芽率为31.1%。同时,还对F1代杂种的形态学和农艺学特性进行了观察,最后还讨论了提高杂交率的方法及伪杂种的问题。 展开更多
关键词 耐旱性 野生稻 远缘杂交 胚胎拯救 远缘杂交 耐旱特性 野生稻 栽培稻 胚胎 ORYZA F1代杂种 水稻品种 成熟种子
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Introgression of Gene for Non-Pollen Type Thermo-Sensitive Genic Male Sterility to Thai Rice Cultivars
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作者 TANEE Sreewongchai WEERACHAI Matthayatthaworn +1 位作者 CHALERMPOL Phumichai prapa sripichitt 《Rice science》 SCIE 2014年第2期123-126,共4页
For the two-line hybrid rice system, pol en sterility is regulated by recessive gene that responds to temperature. The recessive gene controlling thermo-sensitive genetic male sterility (TGMS) is expressed when the ... For the two-line hybrid rice system, pol en sterility is regulated by recessive gene that responds to temperature. The recessive gene controlling thermo-sensitive genetic male sterility (TGMS) is expressed when the plants are grown in conditions with higher or lower critical temperatures. To transfer tgms gene(s) control ing TGMS to Thai rice cultivars by backcross breeding method, a male sterile line was used as a donor parent while Thai rice cultivars ChaiNat 1, PathumThani 1, and SuphanBuri 1 were used as recurrent parents. The BC2F2 lines were developed from backcrossing and selfing. Moreover, the simple sequence repeat (SSR) markers were developed for identifying tgms gene and the linked marker was used for assisting selection in backcrossing. The identification lines were confirmed by pol en observation. The results showed the success of introgression of the tgms gene into Thai rice cultivars. These lines will be tested for combining ability and used as female parent in hybrid rice production in Thailand. 展开更多
关键词 hybrid rice thermo-sensitive genic male sterility non-pollen type molecular marker markerassisted selection marker assisted backcrossing
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与大豆田间老化抗性连锁的分子标记的发掘及辅助选择应用研究(英文)
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作者 叶昌荣 prapa sripichitt +3 位作者 Vipa Hongtrakul Sunanta Juntakool Arom sripichitt Shu Fukai 《大豆科学》 CAS CSCD 北大核心 2007年第6期820-827,共8页
大豆种子成熟至收获期间如遇高温高湿天气,种子活力及活性会急剧下降,这就是所谓的田间老化(field weathering)。田间老化是热带、亚热带地区大豆生产的主要限制因素之一。本研究旨在寻找与田间老化性状相连锁的DNA标记并将其应用于辅... 大豆种子成熟至收获期间如遇高温高湿天气,种子活力及活性会急剧下降,这就是所谓的田间老化(field weathering)。田间老化是热带、亚热带地区大豆生产的主要限制因素之一。本研究旨在寻找与田间老化性状相连锁的DNA标记并将其应用于辅助选择育种。为此,利用修改的培养箱老化法和人工控制老化法对大豆品种Chiangmai 60(敏感),GC10981(抵抗)及其F2群体(139个体)进行了鉴定。在两种处理条件下,F2代群体的种子发芽率及活性均为正态分布,说明大豆种子田间老化抗性受多个基因控制。根据F2代个体的种子发芽率及活性,6个高抗个体及7个高感个体的DNA分别被混合为抗性池和感性池,并利用AFLP标记进行了混合群体分析(BulkSegregant Analysis)。从扩增的2162个标记中,发现了5个可能于大豆种子田间抗性相连锁的片段。通过DNA克隆和测序,设计了5对引物用于从大豆总DNA中扩增相应的片段。其中3对引物扩增的片段差异太小或未能扩增正确大小的片段,没能用于F2群体。引物Eaag/Mcac-233和Eact/Mctt-157能扩增出差异明显的多态性,通过对F2代群体的分析,这2个标记属同一连锁群,遗传距离为25.8c M。QTL分析结果显示有一个QTL位于这两个标记之间,距Eaag/Mcac-233约14c M,可以解释29.7%的变异。用这两对引物对整个F2群体进行筛选,20个个体属于抗性群体,结合抗性鉴定的结果,7个个体被用于与Chiangmai60进行回交。18个BC1F1个体(41·9%)的抗性高于其亲本的平均值。说明这些标记进行可以被用于大豆田间老化抗性的辅助筛选研究。 展开更多
关键词 大豆 田间老化抗性 混合群体分析 数量性状位点 标记辅助选择
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Effect of Spacing on Growth, Biomass Yield and Quality of Leucaena (<i>Leucaena leucocephala</i>(Lam.) de Wit.) for Renewable Energy in Thailand
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作者 Songyos Chotchutima Kunn Kangvansaichol +1 位作者 Sayan Tudsri prapa sripichitt 《Journal of Sustainable Bioenergy Systems》 2013年第1期48-56,共9页
The present study was conducted to determine the effect of spacing on the growth, biomass production and wood quality of leucaena in order to be used as a fuel crop. Leucaena was grown in a field experiment at the Suw... The present study was conducted to determine the effect of spacing on the growth, biomass production and wood quality of leucaena in order to be used as a fuel crop. Leucaena was grown in a field experiment at the Suwanvajokkasikit Research Station, Pak Chong,Nakhon Ratchasima,Thailandin 2006-2010. The experiment was arranged in a randomized complete block design with 4 replications. The treatment consisted of six spacings (1 × 0.25, 1 × 0.5, 1 × 1, 1 × 1.5, 2 × 0.5 and 2 × 1 m). The results showed that spacing had a significant effect on plant height, diameter at breast height, the number of coppice stumps and biomass yield. Wider spacings resulted in greater plant height. The widest spacing (2 × 1 m) exhibited the higher stem diameter and sprout number than the narrow spacing. The narrowest spacing of 1 × 0.25 m spacing produced the highest total dry weight of leaf, woody stem and biomass yield. The spacing did not have an influence on the heating value and the content of H, N, S, Mg, Cl and ash. However, some of the chemical compositions show significant different with different spacings such as C, O, P, K and Ca content. 展开更多
关键词 SPACING Diameter at Breast Height (DBH) BIOMASS Renewable Energy
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Biomass Yield, Chemical Composition and Potential Ethanol Yields of 8 Cultivars of Napiergrass (<i>Pennisetum purpureum</i>Schumach.) Harvested 3-Monthly in Central Thailand
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作者 Kannika Rengsirikul Yasuyuki Ishii +5 位作者 Kunn Kangvansaichol prapa sripichitt Vittaya Punsuvon Pilanee Vaithanomsat Ganda Nakamanee Sayan Tudsri 《Journal of Sustainable Bioenergy Systems》 2013年第2期107-112,共6页
Eight cultivars of napiergrass (Pennisetum purpureum Schumach.), namely Dwarf, Muaklek, Bana, Taiwan A148, Common, Wruk wona, Tifton and Kampheng San, were grown in central Thailand in 2008-2009 and biomass yield, che... Eight cultivars of napiergrass (Pennisetum purpureum Schumach.), namely Dwarf, Muaklek, Bana, Taiwan A148, Common, Wruk wona, Tifton and Kampheng San, were grown in central Thailand in 2008-2009 and biomass yield, chemical composition and theoretical ethanol yield were measured. Harvests were made every 3 months. Biomass yield and cell wall compositions differed significantly (P 0.05) among cultivars. Tifton produced the highest annual biomass yield at 58.3 t/ha followed by Wruk wona (52.1 t/ha), while the lowest yield of 27.1 t/ha was in Dwarf. Biomass yield varied with season with highest yields in May and lowest in February during the dry season. Cell wall concentrations were higher in the tall cultivars than in the short ones (Dwarf and Muaklek) (P 0.05). Theoretical ethanol conversion efficiency ranged from 350 to 460 L/t DM among the cultivars following pretreatment with steam explosion. While a number of cultivars showed significant potential for use as biofuels in central Thailand, Tifton seemed to be the most promising. 展开更多
关键词 BIOENERGY Biomass Yield Cultivar PENNISETUM purpureum Season
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